(Linear) (Six-base) MAPSORT of: KIBB6789.seq  Check: 7150  from: 1  to: 375



KIBB6789.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:      0     344     375 
   Size:        344      31
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      70     111     375 
   Size:         70      41     264

  Fragments arranged by size: 

                 264      70      41
 
BanII G_rGCy'C

Cuts at:      0     294     375 
   Size:        294      81
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     187     375 
   Size:        187     188
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     297     375 
   Size:        297      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     375 
   Size:        247     128
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      81     375 
   Size:         81     294
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     375 
   Size:        314      61
 
Bsp1286I G_dGCh'C

Cuts at:      0     294     375 
   Size:        294      81
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     103     375 
   Size:        103     272
 
BsrDI GCAATG_nn'

Cuts at:      0     320     375 
   Size:        320      55
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     333     375 
   Size:        333      42
 
BtsI GCAGTG_nn'

Cuts at:      0      83     326     375 
   Size:         83     243      49

  Fragments arranged by size: 

                 243      83      49
 
FspI TGC'GCA

Cuts at:      0     316     375 
   Size:        316      59
 
FspAI rTGC'GCAy

Cuts at:      0     316     375 
   Size:        316      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      65      97     375 
   Size:         65      32     278

  Fragments arranged by size: 

                 278      65      32
 
HindIII A'AGCT_T

Cuts at:      0     107     375 
   Size:        107     268
 
MslI CAynn'nnrTG

Cuts at:      0     125     375 
   Size:        125     250
 
MspA1I CmG'CkG

Cuts at:      0     357     375 
   Size:        357      18
 
NspI r_CATG'y

Cuts at:      0     315     375 
   Size:        315      60
 
PstI C_TGCA'G

Cuts at:      0      59      90     362     375 
   Size:         59      31     272      13

  Fragments arranged by size: 

                 272      59      31      13
 
PvuII CAG'CTG

Cuts at:      0     357     375 
   Size:        357      18
 
SfcI C'TryA_G

Cuts at:      0      55      86     187     358     375 
   Size:         55      31     101     171      17

  Fragments arranged by size: 

                 171     101      55      31      17
 
SmlI C'TyrA_G

Cuts at:      0     262     375 
   Size:        262     113
 
StuI AGG'CCT

Cuts at:      0     145     375 
   Size:        145     230
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     121     375 
   Size:        121     254
 

 Enzymes that do cut:

     AccI     AlfI    BanII     BbsI     BmrI    BpuEI    BseRI     BsgI
 Bsp1286I    BspMI    BsrDI    BstXI     BtsI     FspI    FspAI    Hin4I
  HindIII     MslI   MspA1I     NspI     PstI    PvuII     SfcI     SmlI
     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI