(Linear) (Six-base) MAPSORT of: KIBB6787.seq  Check: 6409  from: 1  to: 375



KIBB6787.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     250     375 
   Size:        247       3     125

  Fragments arranged by size: 

                 247     125       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0     361     375 
   Size:        361      14
 
BanII G_rGCy'C

Cuts at:      0     272     315     375 
   Size:        272      43      60

  Fragments arranged by size: 

                 272      60      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      58     375 
   Size:         58     317
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     375 
   Size:        338      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     375 
   Size:        299      76
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     222     375 
   Size:        222     153
 
BseYI C'CCAG_C

Cuts at:      0     207     375 
   Size:        207     168
 
BsiHKAI G_wGCw'C

Cuts at:      0     272     315     375 
   Size:        272      43      60

  Fragments arranged by size: 

                 272      60      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     272     315     375 
   Size:        272      43      60

  Fragments arranged by size: 

                 272      60      43
 
Bsu36I CC'TnA_GG

Cuts at:      0       8     375 
   Size:          8     367
 
EaeI y'GGCC_r

Cuts at:      0      44     248     375 
   Size:         44     204     127

  Fragments arranged by size: 

                 204     127      44
 
EcoICRI GAG'CTC

Cuts at:      0     270     313     375 
   Size:        270      43      62

  Fragments arranged by size: 

                 270      62      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     250     338     375 
   Size:        247       3      88      37

  Fragments arranged by size: 

                 247      88      37       3
 
MscI TGG'CCA

Cuts at:      0     250     375 
   Size:        250     125
 
MspA1I CmG'CkG

Cuts at:      0     211     358     375 
   Size:        211     147      17

  Fragments arranged by size: 

                 211     147      17
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     361     375 
   Size:        361      14
 
PsiI TTA'TAA

Cuts at:      0     202     375 
   Size:        202     173
 
PvuII CAG'CTG

Cuts at:      0     211     358     375 
   Size:        211     147      17

  Fragments arranged by size: 

                 211     147      17
 
SacI G_AGCT'C

Cuts at:      0     272     315     375 
   Size:        272      43      60

  Fragments arranged by size: 

                 272      60      43
 
SmlI C'TyrA_G

Cuts at:      0     314     375 
   Size:        314      61
 
StyI C'CwwG_G

Cuts at:      0     338     375 
   Size:        338      37
 

 Enzymes that do cut:

     AcuI    AlwNI    BanII     BmrI     BpmI    BpuEI     BsaI    BseYI
  BsiHKAI Bsp1286I   Bsu36I     EaeI  EcoICRI  Eco57MI     MscI   MspA1I
    PflMI     PsiI    PvuII     SacI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI