(Linear) (Six-base) MAPSORT of: KIBB6774.seq  Check: 2704  from: 1  to: 453



KIBB6774.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

ApaI G_GGCC'C

Cuts at:      0     367     453 
   Size:        367      86
 
AvaI C'yCGr_G

Cuts at:      0      16     453 
   Size:         16     437
 
BanI G'GyrC_C

Cuts at:      0      70     453 
   Size:         70     383
 
BanII G_rGCy'C

Cuts at:      0      17     367     453 
   Size:         17     350      86

  Fragments arranged by size: 

                 350      86      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     245     453 
   Size:        245     208
 
BglI GCCn_nnn'nGGC

Cuts at:      0     388     453 
   Size:        388      65
 
BlpI GC'TnA_GC

Cuts at:      0     307     453 
   Size:        307     146
 
Bme1580I G_kGCm'C

Cuts at:      0     367     453 
   Size:        367      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     121     132     274     370     417     453 
   Size:         82      39      11     142      96      47      36

  Fragments arranged by size: 

                 142      96      82      47      39      36      11
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     151     181     453 
   Size:        151      30     272

  Fragments arranged by size: 

                 272     151      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     159     271     453 
   Size:        159     112     182

  Fragments arranged by size: 

                 182     159     112
 
BseYI C'CCAG_C

Cuts at:      0     440     453 
   Size:        440      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     453 
   Size:        442      11
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     453 
   Size:         68     385
 
Bsp1286I G_dGCh'C

Cuts at:      0      17      68     367     453 
   Size:         17      51     299      86

  Fragments arranged by size: 

                 299      86      51      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     119     453 
   Size:        119     334
 
BssSI C'ACGA_G

Cuts at:      0     208     453 
   Size:        208     245
 
BtsI GCAGTG_nn'

Cuts at:      0      50     230     299     453 
   Size:         50     180      69     154

  Fragments arranged by size: 

                 180     154      69      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     127     453 
   Size:        127     326
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     121     132     274     370     417     453 
   Size:         82      39      11     142      96      47      36

  Fragments arranged by size: 

                 142      96      82      47      39      36      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0     364     453 
   Size:        364      89
 
HaeII r_GCGC'y

Cuts at:      0       9     453 
   Size:          9     444
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183     232     264     453 
   Size:        151      32      49      32     189

  Fragments arranged by size: 

                 189     151      49      32      32
 
MspA1I CmG'CkG

Cuts at:      0      60     453 
   Size:         60     393
 
NspI r_CATG'y

Cuts at:      0     348     453 
   Size:        348     105
 
PfoI T'CCnGG_A

Cuts at:      0     182     453 
   Size:        182     271
 
PspOMI G'GGCC_C

Cuts at:      0     363     453 
   Size:        363      90
 
PstI C_TGCA'G

Cuts at:      0     225     453 
   Size:        225     228
 
SfcI C'TryA_G

Cuts at:      0     221     453 
   Size:        221     232
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     286     316     453 
   Size:        286      30     137

  Fragments arranged by size: 

                 286     137      30
 
TatI w'GTAC_w

Cuts at:      0     128     453 
   Size:        128     325
 

 Enzymes that do cut:

     ApaI     AvaI     BanI    BanII     BbsI     BglI     BlpI Bme1580I
     BpmI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BssSI     BtsI     EarI  Eco57MI EcoO109I    HaeII    Hin4I   MspA1I
     NspI     PfoI   PspOMI     PstI     SfcI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI