(Linear) (Six-base) MAPSORT of: KIBB6770.seq  Check: 116  from: 1  to: 450



KIBB6770.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AclI AA'CG_TT

Cuts at:      0     377     450 
   Size:        377      73
 
ApoI r'AATT_y

Cuts at:      0     217     450 
   Size:        217     233
 
AvaI C'yCGr_G

Cuts at:      0     179     337     450 
   Size:        179     158     113

  Fragments arranged by size: 

                 179     158     113
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     113     450 
   Size:        113     337
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     380     450 
   Size:        380      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     450 
   Size:        145     305
 
BsrBI CCG'CTC

Cuts at:      0      18     450 
   Size:         18     432
 
BsrDI GCAATG_nn'

Cuts at:      0     259     450 
   Size:        259     191
 
BstYI r'GATC_y

Cuts at:      0      29     450 
   Size:         29     421
 
Bsu36I CC'TnA_GG

Cuts at:      0      34     450 
   Size:         34     416
 
BtsI GCAGTG_nn'

Cuts at:      0     179     450 
   Size:        179     271
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     115     450 
   Size:        115     335
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     211     450 
   Size:        211     239
 
SmlI C'TyrA_G

Cuts at:      0     160     179     450 
   Size:        160      19     271

  Fragments arranged by size: 

                 271     160      19
 
StyI C'CwwG_G

Cuts at:      0       8     450 
   Size:          8     442
 
XhoI C'TCGA_G

Cuts at:      0     179     450 
   Size:        179     271
 

 Enzymes that do cut:

     AclI     ApoI     AvaI     BbsI     BmrI    BpuEI    BsrBI    BsrDI
    BstYI   Bsu36I     BtsI     DrdI     EciI     SmlI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI