(Linear) (Six-base) MAPSORT of: KIBB6764.seq  Check: 9295  from: 1  to: 444



KIBB6764.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     319     444 
   Size:        319     125
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     444 
   Size:        215     229
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     381     411     444 
   Size:        381      30      33

  Fragments arranged by size: 

                 381      33      30
 
BssSI C'ACGA_G

Cuts at:      0     173     444 
   Size:        173     271
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     277     444 
   Size:        277     167
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     303     444 
   Size:        303     141
 
BtsI GCAGTG_nn'

Cuts at:      0     303     444 
   Size:        303     141
 
EaeI y'GGCC_r

Cuts at:      0      77     146     444 
   Size:         77      69     298

  Fragments arranged by size: 

                 298      77      69
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     444 
   Size:        215     229
 
MscI TGG'CCA

Cuts at:      0      79     148     444 
   Size:         79      69     296

  Fragments arranged by size: 

                 296      79      69
 
PstI C_TGCA'G

Cuts at:      0     298     444 
   Size:        298     146
 
SfcI C'TryA_G

Cuts at:      0     111     294     329     444 
   Size:        111     183      35     115

  Fragments arranged by size: 

                 183     115     111      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     369     444 
   Size:        369      75
 

 Enzymes that do cut:

     AlfI     BpmI    BsaXI    BssSI   BstAPI    BtgZI     BtsI     EaeI
  Eco57MI     MscI     PstI     SfcI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI