(Linear) (Six-base) MAPSORT of: KIBB6760.seq  Check: 9933  from: 1  to: 441



KIBB6760.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      73     251     441 
   Size:         73     178     190

  Fragments arranged by size: 

                 190     178      73
 
ApaI G_GGCC'C

Cuts at:      0     118     441 
   Size:        118     323
 
ApoI r'AATT_y

Cuts at:      0     215     441 
   Size:        215     226
 
BamHI G'GATC_C

Cuts at:      0      14      45     441 
   Size:         14      31     396

  Fragments arranged by size: 

                 396      31      14
 
BanI G'GyrC_C

Cuts at:      0     357     441 
   Size:        357      84
 
BanII G_rGCy'C

Cuts at:      0     118     441 
   Size:        118     323
 
Bme1580I G_kGCm'C

Cuts at:      0     118     441 
   Size:        118     323
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      51     441 
   Size:         51     390
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      65     441 
   Size:         65     376
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     441 
   Size:        266     175
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     275     284     441 
   Size:        275       9     157

  Fragments arranged by size: 

                 275     157       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     441 
   Size:        118     323
 
BsrDI GCAATG_nn'

Cuts at:      0     246     441 
   Size:        246     195
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     251     441 
   Size:        251     190
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     111     441 
   Size:        111     330
 
BstYI r'GATC_y

Cuts at:      0      14      45     441 
   Size:         14      31     396

  Fragments arranged by size: 

                 396      31      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     441 
   Size:        266     175
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     134     441 
   Size:        134     307
 
EcoO109I rG'GnC_Cy

Cuts at:      0      70     441 
   Size:         70     371
 
EcoRV GAT'ATC

Cuts at:      0     261     441 
   Size:        261     180
 
HaeII r_GCGC'y

Cuts at:      0       7     441 
   Size:          7     434
 
HincII GTy'rAC

Cuts at:      0     290     441 
   Size:        290     151
 
MslI CAynn'nnrTG

Cuts at:      0     109     441 
   Size:        109     332
 
PpuMI rG'GwC_Cy

Cuts at:      0      70     441 
   Size:         70     371
 
PspOMI G'GGCC_C

Cuts at:      0     114     441 
   Size:        114     327
 
PstI C_TGCA'G

Cuts at:      0      69     441 
   Size:         69     372
 
SfcI C'TryA_G

Cuts at:      0      65     170     441 
   Size:         65     105     271

  Fragments arranged by size: 

                 271     105      65
 
StyI C'CwwG_G

Cuts at:      0     135     441 
   Size:        135     306
 
TatI w'GTAC_w

Cuts at:      0     398     441 
   Size:        398      43
 
Tth111I GACn'n_nGTC

Cuts at:      0     294     441 
   Size:        294     147
 

 Enzymes that do cut:

    AlwNI     ApaI     ApoI    BamHI     BanI    BanII Bme1580I     BmrI
     BplI     BpmI     BsaI Bsp1286I    BsrDI   BstAPI    BstXI    BstYI
  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII   HincII     MslI    PpuMI
   PspOMI     PstI     SfcI     StyI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoRI     FalI     FseI     FspI    FspAI    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI