(Linear) (Six-base) MAPSORT of: KIBB6742.seq  Check: 6895  from: 1  to: 426



KIBB6742.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     276     426 
   Size:        276     150
 
BanII G_rGCy'C

Cuts at:      0     351     426 
   Size:        351      75
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222     426 
   Size:        222     204
 
BglI GCCn_nnn'nGGC

Cuts at:      0      70     426 
   Size:         70     356
 
BlpI GC'TnA_GC

Cuts at:      0       7     426 
   Size:          7     419
 
Bpu10I CC'TnA_GC

Cuts at:      0      46     426 
   Size:         46     380
 
BseYI C'CCAG_C

Cuts at:      0     263     279     426 
   Size:        263      16     147

  Fragments arranged by size: 

                 263     147      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     426 
   Size:        139     287
 
BsiHKAI G_wGCw'C

Cuts at:      0     351     426 
   Size:        351      75
 
Bsp1286I G_dGCh'C

Cuts at:      0     351     426 
   Size:        351      75
 
BsrBI CCG'CTC

Cuts at:      0     342     426 
   Size:        342      84
 
BtgI C'CryG_G

Cuts at:      0      53      59      65     426 
   Size:         53       6       6     361

  Fragments arranged by size: 

                 361      53       6       6
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      33     426 
   Size:         33     393
 
EaeI y'GGCC_r

Cuts at:      0      56      62     426 
   Size:         56       6     364

  Fragments arranged by size: 

                 364      56       6
 
EcoICRI GAG'CTC

Cuts at:      0     349     426 
   Size:        349      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21     426 
   Size:         21     405
 
HaeII r_GCGC'y

Cuts at:      0      53     426 
   Size:         53     373
 
MscI TGG'CCA

Cuts at:      0      58     426 
   Size:         58     368
 
NspI r_CATG'y

Cuts at:      0     162     426 
   Size:        162     264
 
PpuMI rG'GwC_Cy

Cuts at:      0      21     426 
   Size:         21     405
 
PshAI GACnn'nnGTC

Cuts at:      0     122     426 
   Size:        122     304
 
PstI C_TGCA'G

Cuts at:      0      95     426 
   Size:         95     331
 
SacI G_AGCT'C

Cuts at:      0     351     426 
   Size:        351      75
 
ScaI AGT'ACT

Cuts at:      0      32     426 
   Size:         32     394
 
SfcI C'TryA_G

Cuts at:      0      91     426 
   Size:         91     335
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      70     426 
   Size:         70     356
 
StyI C'CwwG_G

Cuts at:      0     237     426 
   Size:        237     189
 
TatI w'GTAC_w

Cuts at:      0      30     426 
   Size:         30     396
 

 Enzymes that do cut:

    AlwNI    BanII     BbsI     BglI     BlpI   Bpu10I    BseYI     BsgI
  BsiHKAI Bsp1286I    BsrBI     BtgI     DrdI     EaeI  EcoICRI EcoO109I
    HaeII     MscI     NspI    PpuMI    PshAI     PstI     SacI     ScaI
     SfcI     SfiI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI