(Linear) (Six-base) MAPSORT of: KIBB6724.seq  Check: 3755  from: 1  to: 405



KIBB6724.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AatII G_ACGT'C

Cuts at:      0      77     405 
   Size:         77     328
 
AccI GT'mk_AC

Cuts at:      0       8     405 
   Size:          8     397
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     405 
   Size:        130     275
 
ApoI r'AATT_y

Cuts at:      0      45     405 
   Size:         45     360
 
BclI T'GATC_A

Cuts at:      0     149     405 
   Size:        149     256
 
BlpI GC'TnA_GC

Cuts at:      0     163     405 
   Size:        163     242
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      26     318     405 
   Size:         26     292      87

  Fragments arranged by size: 

                 292      87      26
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     295     405 
   Size:        295     110
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     373     405 
   Size:        265     108      32

  Fragments arranged by size: 

                 265     108      32
 
BsaHI Gr'CG_yC

Cuts at:      0      74     405 
   Size:         74     331
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     229     405 
   Size:         67     162     176

  Fragments arranged by size: 

                 176     162      67
 
BseYI C'CCAG_C

Cuts at:      0     263     405 
   Size:        263     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     310     331     405 
   Size:        151     159      21      74

  Fragments arranged by size: 

                 159     151      74      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     169     405 
   Size:        169     236
 
Bsp1286I G_dGCh'C

Cuts at:      0     169     405 
   Size:        169     236
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      68     405 
   Size:         68     337
 
BssHII G'CGCG_C

Cuts at:      0      63     405 
   Size:         63     342
 
BstEII G'GTnAC_C

Cuts at:      0     139     405 
   Size:        139     266
 
BstYI r'GATC_y

Cuts at:      0     219     405 
   Size:        219     186
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     265     373     405 
   Size:        130     135     108      32

  Fragments arranged by size: 

                 135     130     108      32
 
FspI TGC'GCA

Cuts at:      0      91     405 
   Size:         91     314
 
HincII GTy'rAC

Cuts at:      0       9      39     405 
   Size:          9      30     366

  Fragments arranged by size: 

                 366      30       9
 
NdeI CA'TA_TG

Cuts at:      0     146     405 
   Size:        146     259
 
SalI G'TCGA_C

Cuts at:      0       7     405 
   Size:          7     398
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     321     353     405 
   Size:        321      32      52

  Fragments arranged by size: 

                 321      52      32
 
ZraI GAC'GTC

Cuts at:      0      75     405 
   Size:         75     330
 

 Enzymes that do cut:

    AatII     AccI     AcuI     ApoI     BclI     BlpI     BmrI     BplI
     BpmI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
   BssHII   BstEII    BstYI  Eco57MI     FspI   HincII     NdeI     SalI
     TstI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI