(Linear) (Six-base) MAPSORT of: KIBB6720.seq  Check: 5327  from: 1  to: 402



KIBB6720.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

BclI T'GATC_A

Cuts at:      0     129     402 
   Size:        129     273
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     402 
   Size:        373      29
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     112     402 
   Size:        112     290
 
BsrFI r'CCGG_y

Cuts at:      0     239     402 
   Size:        239     163
 
BstEII G'GTnAC_C

Cuts at:      0     258     367     402 
   Size:        258     109      35

  Fragments arranged by size: 

                 258     109      35
 
BtgI C'CryG_G

Cuts at:      0     312     402 
   Size:        312      90
 
BtsI GCAGTG_nn'

Cuts at:      0     331     402 
   Size:        331      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     402 
   Size:        373      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     402 
   Size:         66     336
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     402 
   Size:        180     222
 
MspA1I CmG'CkG

Cuts at:      0     314     402 
   Size:        314      88
 
NspI r_CATG'y

Cuts at:      0     247     402 
   Size:        247     155
 
SacII CC_GC'GG

Cuts at:      0     315     402 
   Size:        315      87
 
SexAI A'CCwGG_T

Cuts at:      0     371     402 
   Size:        371      31
 
TatI w'GTAC_w

Cuts at:      0     218     402 
   Size:        218     184
 

 Enzymes that do cut:

     BclI     BpmI    BsmBI    BsrFI   BstEII     BtgI     BtsI  Eco57MI
 EcoO109I     MmeI   MspA1I     NspI    SacII    SexAI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI