Restriction map for
pF1KB6658
(Linear) (Six-base) MAPSORT of: KIBB6658.seq Check: 1201 from: 1 to: 1353
KIBB6658.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 873 1353
Size: 873 480
AatII G_ACGT'C
Cuts at: 0 101 1022 1109 1353
Size: 101 921 87 244
Fragments arranged by size:
921 244 101 87
AccI GT'mk_AC
Cuts at: 0 1022 1353
Size: 1022 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1353
Size: 1088 265
AfeI AGC'GCT
Cuts at: 0 1073 1353
Size: 1073 280
AhdI GACnn_n'nnGTC
Cuts at: 0 57 1110 1353
Size: 57 1053 243
Fragments arranged by size:
1053 243 57
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 1353
Size: 217 32 1104
Fragments arranged by size:
1104 217 32
AlwNI CAG_nnn'CTG
Cuts at: 0 477 693 1353
Size: 477 216 660
Fragments arranged by size:
660 477 216
ApaI G_GGCC'C
Cuts at: 0 31 471 1353
Size: 31 440 882
Fragments arranged by size:
882 440 31
ApaLI G'TGCA_C
Cuts at: 0 1246 1353
Size: 1246 107
AvaI C'yCGr_G
Cuts at: 0 8 445 665 1079 1282 1353
Size: 8 437 220 414 203 71
Fragments arranged by size:
437 414 220 203 71 8
BanI G'GyrC_C
Cuts at: 0 298 1155 1353
Size: 298 857 198
Fragments arranged by size:
857 298 198
BanII G_rGCy'C
Cuts at: 0 31 471 530 626 797 1353
Size: 31 440 59 96 171 556
Fragments arranged by size:
556 440 171 96 59 31
BbeI G_GCGC'C
Cuts at: 0 302 1353
Size: 302 1051
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 606 1353
Size: 93 513 747
Fragments arranged by size:
747 513 93
BbvCI CC'TCA_GC
Cuts at: 0 436 890 1353
Size: 436 454 463
Fragments arranged by size:
463 454 436
BclI T'GATC_A
Cuts at: 0 308 1353
Size: 308 1045
BglII A'GATC_T
Cuts at: 0 383 1353
Size: 383 970
BlpI GC'TnA_GC
Cuts at: 0 79 1353
Size: 79 1274
Bme1580I G_kGCm'C
Cuts at: 0 31 188 471 1160 1237 1250 1353
Size: 31 157 283 689 77 13 103
Fragments arranged by size:
689 283 157 103 77 31 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 558 1353
Size: 558 795
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 1338 1353
Size: 691 647 15
Fragments arranged by size:
691 647 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 928 1249 1308 1353
Size: 838 90 321 59 45
Fragments arranged by size:
838 321 90 59 45
Bpu10I CC'TnA_GC
Cuts at: 0 436 524 890 1353
Size: 436 88 366 463
Fragments arranged by size:
463 436 366 88
BsaI GGTCTCn'nnnn_
Cuts at: 0 1284 1353
Size: 1284 69
BsaAI yAC'GTr
Cuts at: 0 108 1353
Size: 108 1245
BsaHI Gr'CG_yC
Cuts at: 0 98 299 1019 1106 1202 1353
Size: 98 201 720 87 96 151
Fragments arranged by size:
720 201 151 98 96 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 115 142 145 1353
Size: 112 3 27 3 1208
Fragments arranged by size:
1208 112 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 136 580 1353
Size: 88 48 444 773
Fragments arranged by size:
773 444 88 48
BseYI C'CCAG_C
Cuts at: 0 588 1240 1353
Size: 588 652 113
Fragments arranged by size:
652 588 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 664 1293 1353
Size: 394 270 629 60
Fragments arranged by size:
629 394 270 60
BsiHKAI G_wGCw'C
Cuts at: 0 797 1250 1353
Size: 797 453 103
Fragments arranged by size:
797 453 103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 205 1353
Size: 205 1148
Bsp1286I G_dGCh'C
Cuts at: 0 31 188 471 530 626 797 1160 1237
Size: 31 157 283 59 96 171 363 77
Cuts at: 1237 1250 1353
Size: 13 103
Fragments arranged by size:
363 283 171 157 103 96 77 59
31 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 873 1353
Size: 873 480
BsrBI CCG'CTC
Cuts at: 0 254 1353
Size: 254 1099
BsrFI r'CCGG_y
Cuts at: 0 404 1204 1353
Size: 404 800 149
Fragments arranged by size:
800 404 149
BsrGI T'GTAC_A
Cuts at: 0 779 1353
Size: 779 574
BssSI C'ACGA_G
Cuts at: 0 517 1353
Size: 517 836
BstAPI GCAn_nnn'nTGC
Cuts at: 0 369 693 1220 1353
Size: 369 324 527 133
Fragments arranged by size:
527 369 324 133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 147 1353
Size: 39 108 1206
Fragments arranged by size:
1206 108 39
BstYI r'GATC_y
Cuts at: 0 383 704 1353
Size: 383 321 649
Fragments arranged by size:
649 383 321
Bsu36I CC'TnA_GG
Cuts at: 0 1192 1353
Size: 1192 161
BtgI C'CryG_G
Cuts at: 0 167 229 293 483 755 764 1353
Size: 167 62 64 190 272 9 589
Fragments arranged by size:
589 272 190 167 64 62 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 858 1353
Size: 858 495
BtsI GCAGTG_nn'
Cuts at: 0 133 1353
Size: 133 1220
DrdI GACnn_nn'nnGTC
Cuts at: 0 219 1353
Size: 219 1134
EaeI y'GGCC_r
Cuts at: 0 110 200 497 647 800 938 1353
Size: 110 90 297 150 153 138 415
Fragments arranged by size:
415 297 153 150 138 110 90
EcoICRI GAG'CTC
Cuts at: 0 795 1353
Size: 795 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 928 1088 1249 1308 1353
Size: 838 90 160 161 59 45
Fragments arranged by size:
838 161 160 90 59 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 418 1191 1353
Size: 418 773 162
Fragments arranged by size:
773 418 162
EcoO109I rG'GnC_Cy
Cuts at: 0 28 467 771 933 1278 1305 1353
Size: 28 439 304 162 345 27 48
Fragments arranged by size:
439 345 304 162 48 28 27
HaeII r_GCGC'y
Cuts at: 0 302 748 1075 1353
Size: 302 446 327 278
Fragments arranged by size:
446 327 302 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 412 444 1096 1128 1353
Size: 43 32 337 32 652 32 225
Fragments arranged by size:
652 337 225 43 32 32 32
KasI G'GCGC_C
Cuts at: 0 298 1353
Size: 298 1055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 680 1127 1199 1353
Size: 203 477 447 72 154
Fragments arranged by size:
477 447 203 154 72
MscI TGG'CCA
Cuts at: 0 112 649 802 1353
Size: 112 537 153 551
Fragments arranged by size:
551 537 153 112
MslI CAynn'nnrTG
Cuts at: 0 542 655 1269 1353
Size: 542 113 614 84
Fragments arranged by size:
614 542 113 84
NaeI GCC'GGC
Cuts at: 0 406 1206 1353
Size: 406 800 147
Fragments arranged by size:
800 406 147
NarI GG'CG_CC
Cuts at: 0 299 1353
Size: 299 1054
NgoMIV G'CCGG_C
Cuts at: 0 404 1204 1353
Size: 404 800 149
Fragments arranged by size:
800 404 149
NspI r_CATG'y
Cuts at: 0 900 1173 1218 1268 1353
Size: 900 273 45 50 85
Fragments arranged by size:
900 273 85 50 45
PasI CC'CwG_GG
Cuts at: 0 174 1353
Size: 174 1179
PfoI T'CCnGG_A
Cuts at: 0 61 1353
Size: 61 1292
PmlI CAC'GTG
Cuts at: 0 108 1353
Size: 108 1245
PpuMI rG'GwC_Cy
Cuts at: 0 771 933 1278 1353
Size: 771 162 345 75
Fragments arranged by size:
771 345 162 75
PspOMI G'GGCC_C
Cuts at: 0 27 467 1353
Size: 27 440 886
Fragments arranged by size:
886 440 27
PstI C_TGCA'G
Cuts at: 0 278 583 698 1353
Size: 278 305 115 655
Fragments arranged by size:
655 305 278 115
SacI G_AGCT'C
Cuts at: 0 797 1353
Size: 797 556
SanDI GG'GwC_CC
Cuts at: 0 771 1278 1353
Size: 771 507 75
Fragments arranged by size:
771 507 75
SfcI C'TryA_G
Cuts at: 0 274 579 694 1353
Size: 274 305 115 659
Fragments arranged by size:
659 305 274 115
SfoI GGC'GCC
Cuts at: 0 300 1353
Size: 300 1053
SmaI CCC'GGG
Cuts at: 0 10 1353
Size: 10 1343
SmlI C'TyrA_G
Cuts at: 0 665 1353
Size: 665 688
SphI G_CATG'C
Cuts at: 0 1218 1353
Size: 1218 135
StuI AGG'CCT
Cuts at: 0 1186 1353
Size: 1186 167
StyI C'CwwG_G
Cuts at: 0 803 1353
Size: 803 550
TatI w'GTAC_w
Cuts at: 0 779 1353
Size: 779 574
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 112 115 144 147 1353
Size: 112 3 29 3 1206
Fragments arranged by size:
1206 112 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 0 340 637 1353
Size: 340 297 716
Fragments arranged by size:
716 340 297
XhoI C'TCGA_G
Cuts at: 0 665 1353
Size: 665 688
XmaI C'CCGG_G
Cuts at: 0 8 1353
Size: 8 1345
ZraI GAC'GTC
Cuts at: 0 99 1020 1107 1353
Size: 99 921 87 246
Fragments arranged by size:
921 246 99 87
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI
MscI MslI NaeI NarI NgoMIV NspI PasI PfoI
PmlI PpuMI PspOMI PstI SacI SanDI SfcI SfoI
SmaI SmlI SphI StuI StyI TatI TstI Tth111I
XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AleI AlfI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BfrBI BglI BmgBI BmtI BpuEI BsaBI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrDI BssHII BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII EagI EarI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MspA1I NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TaqII XbaI XcmI
XmnI