Restriction map for
pF1KB6656
(Linear) (Six-base) MAPSORT of: KIBB6656.seq Check: 7081 from: 1 to: 1350
KIBB6656.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 0 1184 1350
Size: 1184 166
Acc65I G'GTAC_C
Cuts at: 0 220 1350
Size: 220 1130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 725 1195 1350
Size: 604 121 470 155
Fragments arranged by size:
604 470 155 121
AflIII A'CryG_T
Cuts at: 0 1103 1350
Size: 1103 247
AleI CACnn'nnGTG
Cuts at: 0 112 1350
Size: 112 1238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 760 1350
Size: 760 590
AlwNI CAG_nnn'CTG
Cuts at: 0 81 560 753 1063 1350
Size: 81 479 193 310 287
Fragments arranged by size:
479 310 287 193 81
ApaI G_GGCC'C
Cuts at: 0 567 1350
Size: 567 783
ApoI r'AATT_y
Cuts at: 0 1084 1166 1350
Size: 1084 82 184
Fragments arranged by size:
1084 184 82
AvaI C'yCGr_G
Cuts at: 0 1190 1350
Size: 1190 160
BanI G'GyrC_C
Cuts at: 0 220 1268 1350
Size: 220 1048 82
Fragments arranged by size:
1048 220 82
BanII G_rGCy'C
Cuts at: 0 567 785 1350
Size: 567 218 565
Fragments arranged by size:
567 565 218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 667 701 909 943 1350
Size: 667 34 208 34 407
Fragments arranged by size:
667 407 208 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 170 700 1066 1350
Size: 170 530 366 284
Fragments arranged by size:
530 366 284 170
BlpI GC'TnA_GC
Cuts at: 0 275 412 1350
Size: 275 137 938
Fragments arranged by size:
938 275 137
Bme1580I G_kGCm'C
Cuts at: 0 526 567 1350
Size: 526 41 783
Fragments arranged by size:
783 526 41
BmgBI CAC'GTC
Cuts at: 0 102 624 1104 1350
Size: 102 522 480 246
Fragments arranged by size:
522 480 246 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 121 886 1350
Size: 121 765 464
Fragments arranged by size:
765 464 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1350
Size: 934 416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 1339 1350
Size: 859 480 11
Fragments arranged by size:
859 480 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 932 1006 1350
Size: 932 74 344
Fragments arranged by size:
932 344 74
BsaHI Gr'CG_yC
Cuts at: 0 680 1015 1181 1277 1350
Size: 680 335 166 96 73
Fragments arranged by size:
680 335 166 96 73
BsaWI w'CCGG_w
Cuts at: 0 28 1350
Size: 28 1322
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 784 862 1350
Size: 439 345 78 488
Fragments arranged by size:
488 439 345 78
BseYI C'CCAG_C
Cuts at: 0 180 560 630 855 1350
Size: 180 380 70 225 495
Fragments arranged by size:
495 380 225 180 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1327 1350
Size: 766 561 23
Fragments arranged by size:
766 561 23
BsiHKAI G_wGCw'C
Cuts at: 0 107 328 383 464 1325 1350
Size: 107 221 55 81 861 25
Fragments arranged by size:
861 221 107 81 55 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 92 614 1350
Size: 92 522 736
Fragments arranged by size:
736 522 92
Bsp1286I G_dGCh'C
Cuts at: 0 107 328 383 464 526 567 785 1325
Size: 107 221 55 81 62 41 218 540
Cuts at: 1325 1350
Size: 25
Fragments arranged by size:
540 221 218 107 81 62 55 41
25
BspHI T'CATG_A
Cuts at: 0 716 1253 1350
Size: 716 537 97
Fragments arranged by size:
716 537 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 839 1350
Size: 89 750 511
Fragments arranged by size:
750 511 89
BsrBI CCG'CTC
Cuts at: 0 279 1350
Size: 279 1071
BsrDI GCAATG_nn'
Cuts at: 0 960 1350
Size: 960 390
BssSI C'ACGA_G
Cuts at: 0 1336 1350
Size: 1336 14
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 1350
Size: 81 1269
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1052 1350
Size: 1052 298
Bsu36I CC'TnA_GG
Cuts at: 0 1064 1350
Size: 1064 286
BtgI C'CryG_G
Cuts at: 0 60 249 902 1350
Size: 60 189 653 448
Fragments arranged by size:
653 448 189 60
BtsI GCAGTG_nn'
Cuts at: 0 65 683 1350
Size: 65 618 667
Fragments arranged by size:
667 618 65
DraIII CAC_nnn'GTG
Cuts at: 0 353 1350
Size: 353 997
EaeI y'GGCC_r
Cuts at: 0 22 116 806 980 1350
Size: 22 94 690 174 370
Fragments arranged by size:
690 370 174 94 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 875 1350
Size: 875 475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 725 934 1195 1350
Size: 604 121 209 261 155
Fragments arranged by size:
604 261 209 155 121
EcoO109I rG'GnC_Cy
Cuts at: 0 78 129 564 1068 1350
Size: 78 51 435 504 282
Fragments arranged by size:
504 435 282 78 51
EcoRI G'AATT_C
Cuts at: 0 1166 1350
Size: 1166 184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 72 1350
Size: 72 1278
KpnI G_GTAC'C
Cuts at: 0 224 1350
Size: 224 1126
MscI TGG'CCA
Cuts at: 0 24 808 982 1350
Size: 24 784 174 368
Fragments arranged by size:
784 368 174 24
MslI CAynn'nnrTG
Cuts at: 0 112 1350
Size: 112 1238
MspA1I CmG'CkG
Cuts at: 0 453 557 1350
Size: 453 104 793
Fragments arranged by size:
793 453 104
NcoI C'CATG_G
Cuts at: 0 902 1350
Size: 902 448
PpuMI rG'GwC_Cy
Cuts at: 0 78 129 1350
Size: 78 51 1221
Fragments arranged by size:
1221 78 51
PshAI GACnn'nnGTC
Cuts at: 0 998 1350
Size: 998 352
PspOMI G'GGCC_C
Cuts at: 0 563 1350
Size: 563 787
PstI C_TGCA'G
Cuts at: 0 596 1350
Size: 596 754
PvuII CAG'CTG
Cuts at: 0 453 1350
Size: 453 897
SexAI A'CCwGG_T
Cuts at: 0 740 1350
Size: 740 610
SfcI C'TryA_G
Cuts at: 0 592 1300 1350
Size: 592 708 50
Fragments arranged by size:
708 592 50
SmlI C'TyrA_G
Cuts at: 0 874 1318 1350
Size: 874 444 32
Fragments arranged by size:
874 444 32
StuI AGG'CCT
Cuts at: 0 305 694 1350
Size: 305 389 656
Fragments arranged by size:
656 389 305
StyI C'CwwG_G
Cuts at: 0 125 241 809 902 983 1007 1350
Size: 125 116 568 93 81 24 343
Fragments arranged by size:
568 343 125 116 93 81 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 836 1039 1350
Size: 836 203 311
Fragments arranged by size:
836 311 203
TatI w'GTAC_w
Cuts at: 0 50 431 800 1350
Size: 50 381 369 550
Fragments arranged by size:
550 381 369 50
Tth111I GACn'n_nGTC
Cuts at: 0 1183 1350
Size: 1183 167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 909 1350
Size: 156 753 441
Fragments arranged by size:
753 441 156
XmnI GAAnn'nnTTC
Cuts at: 0 1084 1350
Size: 1084 266
ZraI GAC'GTC
Cuts at: 0 1182 1350
Size: 1182 168
Enzymes that do cut:
AatII Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BcgI BglI BlpI Bme1580I
BmgBI BplI BpmI BpuEI BsaI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstXI Bsu36I BtgI BtsI DraIII
EaeI EciI Eco57MI EcoO109I EcoRI FalI KpnI MscI
MslI MspA1I NcoI PpuMI PshAI PspOMI PstI PvuII
SexAI SfcI SmlI StuI StyI TaqII TatI Tth111I
XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BmrI BmtI
Bpu10I BsaAI BsaBI BsaXI BsiEI BsiWI BsmI BspEI
BsrFI BsrGI BssHII BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoNI
EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XhoI
XmaI