Restriction map for
pF1KB6654
(Linear) (Six-base) MAPSORT of: KIBB6654.seq Check: 870 from: 1 to: 1167
KIBB6654.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 0 83 1167
Size: 83 1084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1167
Size: 85 1082
AflIII A'CryG_T
Cuts at: 0 669 1167
Size: 669 498
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 890 922 1167
Size: 890 32 245
Fragments arranged by size:
890 245 32
AlwNI CAG_nnn'CTG
Cuts at: 0 48 1167
Size: 48 1119
ApoI r'AATT_y
Cuts at: 0 571 1140 1167
Size: 571 569 27
Fragments arranged by size:
571 569 27
AvaI C'yCGr_G
Cuts at: 0 361 1167
Size: 361 806
BanII G_rGCy'C
Cuts at: 0 74 164 1167
Size: 74 90 1003
Fragments arranged by size:
1003 90 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 523 1167
Size: 523 644
BlpI GC'TnA_GC
Cuts at: 0 73 1167
Size: 73 1094
BmrI ACTGGGnnnn_n'
Cuts at: 0 354 633 1012 1167
Size: 354 279 379 155
Fragments arranged by size:
379 354 279 155
Bpu10I CC'TnA_GC
Cuts at: 0 109 1167
Size: 109 1058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1167
Size: 721 446
BsaWI w'CCGG_w
Cuts at: 0 709 1167
Size: 709 458
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 159 328 379 871 1167
Size: 159 169 51 492 296
Fragments arranged by size:
492 296 169 159 51
Bsp1286I G_dGCh'C
Cuts at: 0 74 164 1167
Size: 74 90 1003
Fragments arranged by size:
1003 90 74
BsrBI CCG'CTC
Cuts at: 0 21 113 1167
Size: 21 92 1054
Fragments arranged by size:
1054 92 21
BsrFI r'CCGG_y
Cuts at: 0 102 1167
Size: 102 1065
BstAPI GCAn_nnn'nTGC
Cuts at: 0 48 1167
Size: 48 1119
BstYI r'GATC_y
Cuts at: 0 149 555 1167
Size: 149 406 612
Fragments arranged by size:
612 406 149
BtgI C'CryG_G
Cuts at: 0 164 500 1167
Size: 164 336 667
Fragments arranged by size:
667 336 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 425 1167
Size: 425 742
BtsI GCAGTG_nn'
Cuts at: 0 695 1167
Size: 695 472
EaeI y'GGCC_r
Cuts at: 0 128 1167
Size: 128 1039
EarI CTCTTCn'nnn_
Cuts at: 0 583 1084 1167
Size: 583 501 83
Fragments arranged by size:
583 501 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1167
Size: 85 1082
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 913 1167
Size: 328 585 254
Fragments arranged by size:
585 328 254
EcoO109I rG'GnC_Cy
Cuts at: 0 116 1167
Size: 116 1051
EcoRI G'AATT_C
Cuts at: 0 1140 1167
Size: 1140 27
EcoRV GAT'ATC
Cuts at: 0 267 1167
Size: 267 900
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1095 1167
Size: 1095 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1113 1145 1167
Size: 1113 32 22
Fragments arranged by size:
1113 32 22
HincII GTy'rAC
Cuts at: 0 84 1167
Size: 84 1083
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1167
Size: 1156 11
MscI TGG'CCA
Cuts at: 0 130 1167
Size: 130 1037
MslI CAynn'nnrTG
Cuts at: 0 940 955 1167
Size: 940 15 212
Fragments arranged by size:
940 212 15
MspA1I CmG'CkG
Cuts at: 0 48 63 187 213 476 1167
Size: 48 15 124 26 263 691
Fragments arranged by size:
691 263 124 48 26 15
NaeI GCC'GGC
Cuts at: 0 104 1167
Size: 104 1063
NcoI C'CATG_G
Cuts at: 0 500 1167
Size: 500 667
NgoMIV G'CCGG_C
Cuts at: 0 102 1167
Size: 102 1065
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 1167
Size: 390 777
PfoI T'CCnGG_A
Cuts at: 0 155 1167
Size: 155 1012
PpuMI rG'GwC_Cy
Cuts at: 0 116 1167
Size: 116 1051
PshAI GACnn'nnGTC
Cuts at: 0 121 1167
Size: 121 1046
PstI C_TGCA'G
Cuts at: 0 53 690 1167
Size: 53 637 477
Fragments arranged by size:
637 477 53
PvuII CAG'CTG
Cuts at: 0 48 187 1167
Size: 48 139 980
Fragments arranged by size:
980 139 48
SalI G'TCGA_C
Cuts at: 0 82 1167
Size: 82 1085
SanDI GG'GwC_CC
Cuts at: 0 116 1167
Size: 116 1051
SexAI A'CCwGG_T
Cuts at: 0 836 1167
Size: 836 331
SfcI C'TryA_G
Cuts at: 0 49 686 1167
Size: 49 637 481
Fragments arranged by size:
637 481 49
SmlI C'TyrA_G
Cuts at: 0 736 1167
Size: 736 431
SspI AAT'ATT
Cuts at: 0 1056 1167
Size: 1056 111
StuI AGG'CCT
Cuts at: 0 919 1167
Size: 919 248
StyI C'CwwG_G
Cuts at: 0 329 500 1151 1167
Size: 329 171 651 16
Fragments arranged by size:
651 329 171 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 334 366 1167
Size: 334 32 801
Fragments arranged by size:
801 334 32
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApoI AvaI BanII
BbsI BlpI BmrI Bpu10I BpuEI BsaWI BseRI Bsp1286I
BsrBI BsrFI BstAPI BstYI BtgI BtgZI BtsI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PflMI PfoI PpuMI PshAI PstI PvuII SalI SanDI
SexAI SfcI SmlI SspI StuI StyI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BpmI
BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI