(Linear) (Six-base) MAPSORT of: KIBB6651.seq  Check: 9568  from: 1  to: 1260



KIBB6651.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1260 
   Size:        557     703
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1068    1260 
   Size:       1068     192
 
AlwNI CAG_nnn'CTG

Cuts at:      0     335     787    1260 
   Size:        335     452     473

  Fragments arranged by size: 

                 473     452     335
 
ApoI r'AATT_y

Cuts at:      0     390    1260 
   Size:        390     870
 
BanII G_rGCy'C

Cuts at:      0     940     962    1260 
   Size:        940      22     298

  Fragments arranged by size: 

                 940     298      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     425    1260 
   Size:        425     835
 
BbvCI CC'TCA_GC

Cuts at:      0     610    1260 
   Size:        610     650
 
BclI T'GATC_A

Cuts at:      0       2     162     681     708    1260 
   Size:          2     160     519      27     552

  Fragments arranged by size: 

                 552     519     160      27       2
 
BfrBI ATG'CAT

Cuts at:      0     877    1260 
   Size:        877     383
 
BglII A'GATC_T

Cuts at:      0    1149    1260 
   Size:       1149     111
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     577    1260 
   Size:        577     683
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     636     984    1250    1260 
   Size:        636     348     266      10

  Fragments arranged by size: 

                 636     348     266      10
 
Bpu10I CC'TnA_GC

Cuts at:      0     610    1260 
   Size:        610     650
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116    1035    1260 
   Size:        116     919     225

  Fragments arranged by size: 

                 919     225     116
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     374    1231    1260 
   Size:        374     857      29

  Fragments arranged by size: 

                 857     374      29
 
BseYI C'CCAG_C

Cuts at:      0     335    1180    1260 
   Size:        335     845      80

  Fragments arranged by size: 

                 845     335      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     447     966    1260 
   Size:        447     519     294

  Fragments arranged by size: 

                 519     447     294
 
BsiHKAI G_wGCw'C

Cuts at:      0     940    1260 
   Size:        940     320
 
BsmI GAATG_Cn'

Cuts at:      0     547     877    1260 
   Size:        547     330     383

  Fragments arranged by size: 

                 547     383     330
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     763    1186    1260 
   Size:        763     423      74

  Fragments arranged by size: 

                 763     423      74
 
Bsp1286I G_dGCh'C

Cuts at:      0     940     962    1260 
   Size:        940      22     298

  Fragments arranged by size: 

                 940     298      22
 
BsrDI GCAATG_nn'

Cuts at:      0     801    1260 
   Size:        801     459
 
BstYI r'GATC_y

Cuts at:      0    1149    1260 
   Size:       1149     111
 
Bsu36I CC'TnA_GG

Cuts at:      0     491    1260 
   Size:        491     769
 
BtsI GCAGTG_nn'

Cuts at:      0     459     938    1260 
   Size:        459     479     322

  Fragments arranged by size: 

                 479     459     322
 
EaeI y'GGCC_r

Cuts at:      0     326    1260 
   Size:        326     934
 
EcoICRI GAG'CTC

Cuts at:      0     938    1260 
   Size:        938     322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     636     984    1250    1260 
   Size:        557      79     348     266      10

  Fragments arranged by size: 

                 557     348     266      79      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0     674    1260 
   Size:        674     586
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     472    1260 
   Size:        472     788
 
HincII GTy'rAC

Cuts at:      0     645    1260 
   Size:        645     615
 
HpaI GTT'AAC

Cuts at:      0     645    1260 
   Size:        645     615
 
MfeI C'AATT_G

Cuts at:      0     383    1260 
   Size:        383     877
 
MscI TGG'CCA

Cuts at:      0     328    1260 
   Size:        328     932
 
MspA1I CmG'CkG

Cuts at:      0     335     687    1260 
   Size:        335     352     573

  Fragments arranged by size: 

                 573     352     335
 
NsiI A_TGCA'T

Cuts at:      0     879    1260 
   Size:        879     381
 
NspI r_CATG'y

Cuts at:      0      19     587    1260 
   Size:         19     568     673

  Fragments arranged by size: 

                 673     568      19
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     335    1260 
   Size:        335     925
 
PfoI T'CCnGG_A

Cuts at:      0     372    1260 
   Size:        372     888
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     301     333    1260 
   Size:        301      32     927

  Fragments arranged by size: 

                 927     301      32
 
PstI C_TGCA'G

Cuts at:      0     308    1260 
   Size:        308     952
 
PvuII CAG'CTG

Cuts at:      0     335     687    1260 
   Size:        335     352     573

  Fragments arranged by size: 

                 573     352     335
 
SacI G_AGCT'C

Cuts at:      0     940    1260 
   Size:        940     320
 
SfcI C'TryA_G

Cuts at:      0     304     413     516    1260 
   Size:        304     109     103     744

  Fragments arranged by size: 

                 744     304     109     103
 
SmlI C'TyrA_G

Cuts at:      0     131    1014    1260 
   Size:        131     883     246

  Fragments arranged by size: 

                 883     246     131
 
SphI G_CATG'C

Cuts at:      0     587    1260 
   Size:        587     673
 
XbaI T'CTAG_A

Cuts at:      0     186    1260 
   Size:        186    1074
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApoI    BanII     BbsI    BbvCI     BclI
    BfrBI    BglII     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrDI    BstYI   Bsu36I
     BtsI     EaeI  EcoICRI  Eco57MI EcoO109I     FalI   HincII     HpaI
     MfeI     MscI   MspA1I     NsiI     NspI    PflMI     PfoI     PpiI
     PstI    PvuII     SacI     SfcI     SmlI     SphI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     KasI
     KpnI     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI