Restriction map for
pF1KB6645
(Linear) (Six-base) MAPSORT of: KIBB6645.seq Check: 865 from: 1 to: 1257
KIBB6645.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 241 1257
Size: 241 1016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1005 1257
Size: 160 845 252
Fragments arranged by size:
845 252 160
AflIII A'CryG_T
Cuts at: 0 125 1257
Size: 125 1132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 847 1168 1257
Size: 847 321 89
Fragments arranged by size:
847 321 89
BanI G'GyrC_C
Cuts at: 0 517 633 726 1257
Size: 517 116 93 531
Fragments arranged by size:
531 517 116 93
BanII G_rGCy'C
Cuts at: 0 221 1257
Size: 221 1036
BbsI GAAGACnn'nnnn_
Cuts at: 0 534 598 979 997 1257
Size: 534 64 381 18 260
Fragments arranged by size:
534 381 260 64 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1104 1138 1257
Size: 1104 34 119
Fragments arranged by size:
1104 119 34
BglII A'GATC_T
Cuts at: 0 1118 1257
Size: 1118 139
Bme1580I G_kGCm'C
Cuts at: 0 192 845 1147 1257
Size: 192 653 302 110
Fragments arranged by size:
653 302 192 110
BmgBI CAC'GTC
Cuts at: 0 903 1257
Size: 903 354
BmrI ACTGGGnnnn_n'
Cuts at: 0 1194 1257
Size: 1194 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1257
Size: 895 362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 946 1198 1257
Size: 874 72 252 59
Fragments arranged by size:
874 252 72 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 568 767 1257
Size: 568 199 490
Fragments arranged by size:
568 490 199
BsaAI yAC'GTr
Cuts at: 0 927 1257
Size: 927 330
BsaBI GATnn'nnATC
Cuts at: 0 673 1257
Size: 673 584
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 679 709 1257
Size: 112 30 537 30 548
Fragments arranged by size:
548 537 112 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 412 778 811 1237 1240 1257
Size: 409 3 366 33 426 3 17
Fragments arranged by size:
426 409 366 33 17 3 3
BseYI C'CCAG_C
Cuts at: 0 106 214 810 1092 1257
Size: 106 108 596 282 165
Fragments arranged by size:
596 282 165 108 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 1257
Size: 807 450
BsiEI CG_ry'CG
Cuts at: 0 851 1257
Size: 851 406
BsiWI C'GTAC_G
Cuts at: 0 923 1257
Size: 923 334
BsmI GAATG_Cn'
Cuts at: 0 315 1257
Size: 315 942
Bsp1286I G_dGCh'C
Cuts at: 0 192 221 845 1147 1257
Size: 192 29 624 302 110
Fragments arranged by size:
624 302 192 110 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 241 785 1257
Size: 241 544 472
Fragments arranged by size:
544 472 241
BsrDI GCAATG_nn'
Cuts at: 0 200 1140 1257
Size: 200 940 117
Fragments arranged by size:
940 200 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 257 1257
Size: 257 1000
BstEII G'GTnAC_C
Cuts at: 0 1068 1257
Size: 1068 189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 207 649 1257
Size: 207 442 608
Fragments arranged by size:
608 442 207
BstYI r'GATC_y
Cuts at: 0 932 1031 1118 1257
Size: 932 99 87 139
Fragments arranged by size:
932 139 99 87
Bsu36I CC'TnA_GG
Cuts at: 0 1220 1257
Size: 1220 37
BtgI C'CryG_G
Cuts at: 0 77 845 1139 1257
Size: 77 768 294 118
Fragments arranged by size:
768 294 118 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1179 1257
Size: 1179 78
DrdI GACnn_nn'nnGTC
Cuts at: 0 532 1257
Size: 532 725
EaeI y'GGCC_r
Cuts at: 0 671 750 848 1175 1257
Size: 671 79 98 327 82
Fragments arranged by size:
671 327 98 82 79
EagI C'GGCC_G
Cuts at: 0 848 1257
Size: 848 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 895 1005 1257
Size: 160 735 110 252
Fragments arranged by size:
735 252 160 110
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 1257
Size: 373 884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 192 1257
Size: 192 1065
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 1257
Size: 679 32 546
Fragments arranged by size:
679 546 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1257
Size: 1190 67
MscI TGG'CCA
Cuts at: 0 673 1177 1257
Size: 673 504 80
Fragments arranged by size:
673 504 80
MslI CAynn'nnrTG
Cuts at: 0 205 319 1257
Size: 205 114 938
Fragments arranged by size:
938 205 114
NspI r_CATG'y
Cuts at: 0 129 1173 1257
Size: 129 1044 84
Fragments arranged by size:
1044 129 84
PasI CC'CwG_GG
Cuts at: 0 185 1257
Size: 185 1072
PciI A'CATG_T
Cuts at: 0 125 1257
Size: 125 1132
PflMI CCAn_nnn'nTGG
Cuts at: 0 1065 1257
Size: 1065 192
PfoI T'CCnGG_A
Cuts at: 0 872 1257
Size: 872 385
PshAI GACnn'nnGTC
Cuts at: 0 580 1257
Size: 580 677
PstI C_TGCA'G
Cuts at: 0 431 485 1257
Size: 431 54 772
Fragments arranged by size:
772 431 54
SfcI C'TryA_G
Cuts at: 0 427 481 1257
Size: 427 54 776
Fragments arranged by size:
776 427 54
SmlI C'TyrA_G
Cuts at: 0 889 961 1213 1257
Size: 889 72 252 44
Fragments arranged by size:
889 252 72 44
StuI AGG'CCT
Cuts at: 0 368 1257
Size: 368 889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 1257
Size: 75 1182
XmnI GAAnn'nnTTC
Cuts at: 0 10 151 1257
Size: 10 141 1106
Fragments arranged by size:
1106 141 10
Enzymes that do cut:
AarI AcuI AflIII AlfI BanI BanII BbsI BcgI
BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI
Bsp1286I BspMI BsrDI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI DrdI EaeI EagI Eco57MI EcoNI FalI
Hin4I MmeI MscI MslI NspI PasI PciI PflMI
PfoI PshAI PstI SfcI SmlI StuI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI
BclI BfrBI BglI BlpI BmtI BplI Bpu10I BsaHI
BsaWI BsiHKAI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstZ17I BtsI ClaI CspCI DraI
DraIII EarI EciI EcoICRI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI ZraI