Restriction map for
pF1KB6632
(Linear) (Six-base) MAPSORT of: KIBB6632.seq Check: 5433 from: 1 to: 1320
KIBB6632.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 321 375 1154 1320
Size: 321 54 779 166
Fragments arranged by size:
779 321 166 54
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 862 1320
Size: 184 678 458
Fragments arranged by size:
678 458 184
AfeI AGC'GCT
Cuts at: 0 18 1320
Size: 18 1302
AflIII A'CryG_T
Cuts at: 0 263 926 1320
Size: 263 663 394
Fragments arranged by size:
663 394 263
AlwNI CAG_nnn'CTG
Cuts at: 0 844 1320
Size: 844 476
ApoI r'AATT_y
Cuts at: 0 935 1320
Size: 935 385
AvaI C'yCGr_G
Cuts at: 0 135 712 1320
Size: 135 577 608
Fragments arranged by size:
608 577 135
BamHI G'GATC_C
Cuts at: 0 602 798 1320
Size: 602 196 522
Fragments arranged by size:
602 522 196
BanI G'GyrC_C
Cuts at: 0 37 252 289 643 673 777 1123 1320
Size: 37 215 37 354 30 104 346 197
Fragments arranged by size:
354 346 215 197 104 37 37 30
BanII G_rGCy'C
Cuts at: 0 104 1320
Size: 104 1216
BbeI G_GCGC'C
Cuts at: 0 41 1320
Size: 41 1279
BbsI GAAGACnn'nnnn_
Cuts at: 0 229 1320
Size: 229 1091
BbvCI CC'TCA_GC
Cuts at: 0 1151 1232 1280 1320
Size: 1151 81 48 40
Fragments arranged by size:
1151 81 48 40
BglI GCCn_nnn'nGGC
Cuts at: 0 88 1320
Size: 88 1232
BglII A'GATC_T
Cuts at: 0 1304 1320
Size: 1304 16
BlpI GC'TnA_GC
Cuts at: 0 20 1320
Size: 20 1300
Bme1580I G_kGCm'C
Cuts at: 0 134 257 678 1128 1320
Size: 134 123 421 450 192
Fragments arranged by size:
450 421 192 134 123
BmgBI CAC'GTC
Cuts at: 0 927 1320
Size: 927 393
BmrI ACTGGGnnnn_n'
Cuts at: 0 295 1277 1320
Size: 295 982 43
Fragments arranged by size:
982 295 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 226 412 751 821 875 1026 1320
Size: 118 108 186 339 70 54 151 294
Fragments arranged by size:
339 294 186 151 118 108 70 54
Bpu10I CC'TnA_GC
Cuts at: 0 1151 1226 1232 1280 1320
Size: 1151 75 6 48 40
Fragments arranged by size:
1151 75 48 40 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 1107 1320
Size: 1107 213
BsaHI Gr'CG_yC
Cuts at: 0 11 38 1320
Size: 11 27 1282
Fragments arranged by size:
1282 27 11
BsaWI w'CCGG_w
Cuts at: 0 599 1320
Size: 599 721
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 519 549 1024 1054 1320
Size: 519 30 475 30 266
Fragments arranged by size:
519 475 266 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 777 863 1249 1320
Size: 777 86 386 71
Fragments arranged by size:
777 386 86 71
BseYI C'CCAG_C
Cuts at: 0 78 83 256 619 823 973 1320
Size: 78 5 173 363 204 150 347
Fragments arranged by size:
363 347 204 173 150 78 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 916 1320
Size: 85 831 404
Fragments arranged by size:
831 404 85
BsiHKAI G_wGCw'C
Cuts at: 0 837 1320
Size: 837 483
Bsp1286I G_dGCh'C
Cuts at: 0 104 134 257 678 837 1128 1320
Size: 104 30 123 421 159 291 192
Fragments arranged by size:
421 291 192 159 123 104 30
BspHI T'CATG_A
Cuts at: 0 465 1056 1320
Size: 465 591 264
Fragments arranged by size:
591 465 264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 321 375 1154 1320
Size: 321 54 779 166
Fragments arranged by size:
779 321 166 54
BsrBI CCG'CTC
Cuts at: 0 1062 1320
Size: 1062 258
BsrDI GCAATG_nn'
Cuts at: 0 963 1320
Size: 963 357
BsrFI r'CCGG_y
Cuts at: 0 379 1320
Size: 379 941
BstYI r'GATC_y
Cuts at: 0 602 798 1304 1320
Size: 602 196 506 16
Fragments arranged by size:
602 506 196 16
Bsu36I CC'TnA_GG
Cuts at: 0 1020 1320
Size: 1020 300
BtgI C'CryG_G
Cuts at: 0 57 791 1320
Size: 57 734 529
Fragments arranged by size:
734 529 57
DraIII CAC_nnn'GTG
Cuts at: 0 63 673 1320
Size: 63 610 647
Fragments arranged by size:
647 610 63
EaeI y'GGCC_r
Cuts at: 0 273 1320
Size: 273 1047
EarI CTCTTCn'nnn_
Cuts at: 0 881 990 1166 1320
Size: 881 109 176 154
Fragments arranged by size:
881 176 154 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1153 1197 1320
Size: 1153 44 123
Fragments arranged by size:
1153 123 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 184 226 412 751 821 862 875
Size: 118 66 42 186 339 70 41 13
Cuts at: 875 1026 1320
Size: 151 294
Fragments arranged by size:
339 294 186 151 118 70 66 42
41 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 772 1320
Size: 772 548
EcoO109I rG'GnC_Cy
Cuts at: 0 279 765 1257 1320
Size: 279 486 492 63
Fragments arranged by size:
492 486 279 63
HaeII r_GCGC'y
Cuts at: 0 20 41 1320
Size: 20 21 1279
Fragments arranged by size:
1279 21 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 519 551 594 626 1024 1056 1189 1221
Size: 519 32 43 32 398 32 133 32
Cuts at: 1221 1320
Size: 99
Fragments arranged by size:
519 398 133 99 43 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 37 1320
Size: 37 1283
MslI CAynn'nnrTG
Cuts at: 0 232 1320
Size: 232 1088
MspA1I CmG'CkG
Cuts at: 0 1009 1155 1320
Size: 1009 146 165
Fragments arranged by size:
1009 165 146
NaeI GCC'GGC
Cuts at: 0 381 1320
Size: 381 939
NarI GG'CG_CC
Cuts at: 0 38 1320
Size: 38 1282
NcoI C'CATG_G
Cuts at: 0 791 1320
Size: 791 529
NgoMIV G'CCGG_C
Cuts at: 0 379 1320
Size: 379 941
NspI r_CATG'y
Cuts at: 0 267 343 1320
Size: 267 76 977
Fragments arranged by size:
977 267 76
PciI A'CATG_T
Cuts at: 0 263 1320
Size: 263 1057
PflMI CCAn_nnn'nTGG
Cuts at: 0 864 968 1198 1320
Size: 864 104 230 122
Fragments arranged by size:
864 230 122 104
PfoI T'CCnGG_A
Cuts at: 0 428 920 1109 1320
Size: 428 492 189 211
Fragments arranged by size:
492 428 211 189
PspXI vC'TCGA_Gb
Cuts at: 0 135 1320
Size: 135 1185
PstI C_TGCA'G
Cuts at: 0 310 776 1185 1320
Size: 310 466 409 135
Fragments arranged by size:
466 409 310 135
PvuII CAG'CTG
Cuts at: 0 1009 1155 1320
Size: 1009 146 165
Fragments arranged by size:
1009 165 146
SfcI C'TryA_G
Cuts at: 0 306 772 1181 1320
Size: 306 466 409 139
Fragments arranged by size:
466 409 306 139
SfoI GGC'GCC
Cuts at: 0 39 1320
Size: 39 1281
SmlI C'TyrA_G
Cuts at: 0 135 1320
Size: 135 1185
StyI C'CwwG_G
Cuts at: 0 791 1320
Size: 791 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 1320
Size: 277 1043
XhoI C'TCGA_G
Cuts at: 0 135 1320
Size: 135 1185
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstYI Bsu36I BtgI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PspXI PstI PvuII SfcI SfoI SmlI
StyI TaqII XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BmtI BplI BpuEI
BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NdeI NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI