(Linear) (Six-base) MAPSORT of: KIBB6623.seq  Check: 2236  from: 1  to: 1239



KIBB6623.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     313    1239 
   Size:        128     185     926

  Fragments arranged by size: 

                 926     185     128
 
ApoI r'AATT_y

Cuts at:      0      75     784    1239 
   Size:         75     709     455

  Fragments arranged by size: 

                 709     455      75
 
BanII G_rGCy'C

Cuts at:      0      45     143     346    1239 
   Size:         45      98     203     893

  Fragments arranged by size: 

                 893     203      98      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     331     598     745     827    1044    1160    1239 
   Size:        331     267     147      82     217     116      79

  Fragments arranged by size: 

                 331     267     217     147     116      82      79
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      43     162    1239 
   Size:         43     119    1077

  Fragments arranged by size: 

                1077     119      43
 
BglI GCCn_nnn'nGGC

Cuts at:      0     226    1239 
   Size:        226    1013
 
Bme1580I G_kGCm'C

Cuts at:      0    1063    1239 
   Size:       1063     176
 
BmgBI CAC'GTC

Cuts at:      0     165    1239 
   Size:        165    1074
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     204    1239 
   Size:        204    1035
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1184    1239 
   Size:       1184      55
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1239 
   Size:        799     440
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     877     901    1239 
   Size:        877      24     338

  Fragments arranged by size: 

                 877     338      24
 
BsaWI w'CCGG_w

Cuts at:      0    1199    1239 
   Size:       1199      40
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1117    1239 
   Size:       1117     122
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     143     346    1063    1239 
   Size:         45      98     203     717     176

  Fragments arranged by size: 

                 717     203     176      98      45
 
BspEI T'CCGG_A

Cuts at:      0    1199    1239 
   Size:       1199      40
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     611    1239 
   Size:        611     628
 
BsrDI GCAATG_nn'

Cuts at:      0     512    1239 
   Size:        512     727
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     525    1239 
   Size:        336     189     714

  Fragments arranged by size: 

                 714     336     189
 
BtgI C'CryG_G

Cuts at:      0    1016    1239 
   Size:       1016     223
 
EaeI y'GGCC_r

Cuts at:      0     218    1239 
   Size:        218    1021
 
EarI CTCTTCn'nnn_

Cuts at:      0     896    1239 
   Size:        896     343
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     313     799    1239 
   Size:        128     185     486     440

  Fragments arranged by size: 

                 486     440     185     128
 
HaeII r_GCGC'y

Cuts at:      0     912    1239 
   Size:        912     327
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     376     408     952     984    1239 
   Size:        376      32     544      32     255

  Fragments arranged by size: 

                 544     376     255      32      32
 
HincII GTy'rAC

Cuts at:      0     369    1239 
   Size:        369     870
 
HindIII A'AGCT_T

Cuts at:      0     253    1239 
   Size:        253     986
 
MfeI C'AATT_G

Cuts at:      0     203    1239 
   Size:        203    1036
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     962     995    1239 
   Size:        962      33     244

  Fragments arranged by size: 

                 962     244      33
 
MscI TGG'CCA

Cuts at:      0     220    1239 
   Size:        220    1019
 
MslI CAynn'nnrTG

Cuts at:      0      31    1021    1239 
   Size:         31     990     218

  Fragments arranged by size: 

                 990     218      31
 
NcoI C'CATG_G

Cuts at:      0    1016    1239 
   Size:       1016     223
 
PasI CC'CwG_GG

Cuts at:      0      45    1239 
   Size:         45    1194
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     216    1239 
   Size:        216    1023
 
PfoI T'CCnGG_A

Cuts at:      0     953    1239 
   Size:        953     286
 
PsiI TTA'TAA

Cuts at:      0     314    1239 
   Size:        314     925
 
PstI C_TGCA'G

Cuts at:      0     704    1239 
   Size:        704     535
 
SfcI C'TryA_G

Cuts at:      0     700    1239 
   Size:        700     539
 
SmlI C'TyrA_G

Cuts at:      0     892     916    1239 
   Size:        892      24     323

  Fragments arranged by size: 

                 892     323      24
 
StyI C'CwwG_G

Cuts at:      0     224     329    1016    1239 
   Size:        224     105     687     223

  Fragments arranged by size: 

                 687     224     223     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     102     197    1239 
   Size:        102      95    1042

  Fragments arranged by size: 

                1042     102      95
 
XmnI GAAnn'nnTTC

Cuts at:      0     113    1239 
   Size:        113    1126
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BbsI    BciVI     BglI Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI    BsaWI    BseRI Bsp1286I    BspEI
    BspMI    BsrDI    BstXI     BtgI     EaeI     EarI  Eco57MI    HaeII
    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI     NcoI
     PasI    PflMI     PfoI     PsiI     PstI     SfcI     SmlI     StyI
    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI     BmtI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI