Restriction map for
pF1KB6616
(Linear) (Six-base) MAPSORT of: KIBB6616.seq Check: 54 from: 1 to: 1299
KIBB6616.orf
With 182 enzymes: SgfI *
June 30, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 634 1299
Size: 634 665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1299
Size: 968 331
AflIII A'CryG_T
Cuts at: 0 68 1299
Size: 68 1231
AgeI A'CCGG_T
Cuts at: 0 556 989 1299
Size: 556 433 310
Fragments arranged by size:
556 433 310
AhdI GACnn_n'nnGTC
Cuts at: 0 714 1299
Size: 714 585
AlwNI CAG_nnn'CTG
Cuts at: 0 24 1299
Size: 24 1275
ApaI G_GGCC'C
Cuts at: 0 1230 1299
Size: 1230 69
ApaLI G'TGCA_C
Cuts at: 0 517 1299
Size: 517 782
ApoI r'AATT_y
Cuts at: 0 293 1299
Size: 293 1006
AvaI C'yCGr_G
Cuts at: 0 664 822 1299
Size: 664 158 477
Fragments arranged by size:
664 477 158
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 456 489 1299
Size: 456 33 810
Fragments arranged by size:
810 456 33
BamHI G'GATC_C
Cuts at: 0 260 1299
Size: 260 1039
BanI G'GyrC_C
Cuts at: 0 562 1011 1101 1299
Size: 562 449 90 198
Fragments arranged by size:
562 449 198 90
BanII G_rGCy'C
Cuts at: 0 646 976 1230 1299
Size: 646 330 254 69
Fragments arranged by size:
646 330 254 69
BglI GCCn_nnn'nGGC
Cuts at: 0 1246 1299
Size: 1246 53
BlpI GC'TnA_GC
Cuts at: 0 1198 1299
Size: 1198 101
Bme1580I G_kGCm'C
Cuts at: 0 521 962 1106 1230 1299
Size: 521 441 144 124 69
Fragments arranged by size:
521 441 144 124 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 439 1299
Size: 439 860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1299
Size: 511 788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 889 1299
Size: 223 666 410
Fragments arranged by size:
666 410 223
BsaWI w'CCGG_w
Cuts at: 0 556 926 989 1299
Size: 556 370 63 310
Fragments arranged by size:
556 370 310 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 676 706 1299
Size: 199 30 447 30 593
Fragments arranged by size:
593 447 199 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 321 1138 1299
Size: 321 817 161
Fragments arranged by size:
817 321 161
BseYI C'CCAG_C
Cuts at: 0 24 162 721 1299
Size: 24 138 559 578
Fragments arranged by size:
578 559 138 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 586 1015 1299
Size: 237 349 429 284
Fragments arranged by size:
429 349 284 237
BsiHKAI G_wGCw'C
Cuts at: 0 521 1204 1299
Size: 521 683 95
Fragments arranged by size:
683 521 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 202 1299
Size: 202 1097
Bsp1286I G_dGCh'C
Cuts at: 0 521 646 962 976 1106 1204 1230 1299
Size: 521 125 316 14 130 98 26 69
Fragments arranged by size:
521 316 130 125 98 69 26 14
BspEI T'CCGG_A
Cuts at: 0 926 1299
Size: 926 373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 506 551 634 860 1037 1299
Size: 167 339 45 83 226 177 262
Fragments arranged by size:
339 262 226 177 167 83 45
BsrBI CCG'CTC
Cuts at: 0 843 1299
Size: 843 456
BsrFI r'CCGG_y
Cuts at: 0 556 879 989 1274 1299
Size: 556 323 110 285 25
Fragments arranged by size:
556 323 285 110 25
BstAPI GCAn_nnn'nTGC
Cuts at: 0 414 1250 1299
Size: 414 836 49
Fragments arranged by size:
836 414 49
BstBI TT'CG_AA
Cuts at: 0 764 1299
Size: 764 535
BstEII G'GTnAC_C
Cuts at: 0 726 1299
Size: 726 573
BstXI CCAn_nnnn'nTGG
Cuts at: 0 681 1299
Size: 681 618
BstYI r'GATC_y
Cuts at: 0 233 260 905 1268 1299
Size: 233 27 645 363 31
Fragments arranged by size:
645 363 233 31 27
BtgI C'CryG_G
Cuts at: 0 459 746 1299
Size: 459 287 553
Fragments arranged by size:
553 459 287
BtsI GCAGTG_nn'
Cuts at: 0 598 1299
Size: 598 701
EaeI y'GGCC_r
Cuts at: 0 590 881 1299
Size: 590 291 418
Fragments arranged by size:
590 418 291
EarI CTCTTCn'nnn_
Cuts at: 0 353 1063 1115 1299
Size: 353 710 52 184
Fragments arranged by size:
710 353 184 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 631 1231 1245 1261 1299
Size: 631 600 14 16 38
Fragments arranged by size:
631 600 38 16 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 968 1299
Size: 511 457 331
Fragments arranged by size:
511 457 331
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1159 1299
Size: 1159 140
EcoO109I rG'GnC_Cy
Cuts at: 0 156 1026 1053 1113 1152 1227 1299
Size: 156 870 27 60 39 75 72
Fragments arranged by size:
870 156 75 72 60 39 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 883 1299
Size: 883 416
HaeII r_GCGC'y
Cuts at: 0 1149 1299
Size: 1149 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 676 700 708 732 1299
Size: 37 32 607 24 8 24 567
Fragments arranged by size:
607 567 37 32 24 24 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1299
Size: 602 697
MslI CAynn'nnrTG
Cuts at: 0 323 1006 1299
Size: 323 683 293
Fragments arranged by size:
683 323 293
MspA1I CmG'CkG
Cuts at: 0 24 336 477 920 1197 1299
Size: 24 312 141 443 277 102
Fragments arranged by size:
443 312 277 141 102 24
NaeI GCC'GGC
Cuts at: 0 1276 1299
Size: 1276 23
NgoMIV G'CCGG_C
Cuts at: 0 1274 1299
Size: 1274 25
NspI r_CATG'y
Cuts at: 0 33 408 1299
Size: 33 375 891
Fragments arranged by size:
891 375 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 1299
Size: 339 960
PpuMI rG'GwC_Cy
Cuts at: 0 156 1026 1053 1113 1299
Size: 156 870 27 60 186
Fragments arranged by size:
870 186 156 60 27
PspOMI G'GGCC_C
Cuts at: 0 1226 1299
Size: 1226 73
PvuII CAG'CTG
Cuts at: 0 24 336 477 920 1197 1299
Size: 24 312 141 443 277 102
Fragments arranged by size:
443 312 277 141 102 24
ScaI AGT'ACT
Cuts at: 0 391 1299
Size: 391 908
SgrAI Cr'CCGG_yG
Cuts at: 0 556 1299
Size: 556 743
SmaI CCC'GGG
Cuts at: 0 824 1299
Size: 824 475
SmlI C'TyrA_G
Cuts at: 0 238 868 1299
Size: 238 630 431
Fragments arranged by size:
630 431 238
SphI G_CATG'C
Cuts at: 0 408 1299
Size: 408 891
StuI AGG'CCT
Cuts at: 0 1249 1299
Size: 1249 50
StyI C'CwwG_G
Cuts at: 0 983 1160 1299
Size: 983 177 139
Fragments arranged by size:
983 177 139
TatI w'GTAC_w
Cuts at: 0 389 1299
Size: 389 910
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1299
Size: 199 32 1068
Fragments arranged by size:
1068 199 32
XmaI C'CCGG_G
Cuts at: 0 822 1299
Size: 822 477
XmnI GAAnn'nnTTC
Cuts at: 0 439 1299
Size: 439 860
Enzymes that do cut:
AarI AcuI AflIII AgeI AhdI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BglI BlpI
Bme1580I BmrI BpmI BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BstAPI BstBI BstEII BstXI BstYI BtgI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MmeI MslI MspA1I NaeI NgoMIV NspI PflMI PpuMI
PspOMI PvuII ScaI SgrAI SmaI SmlI SphI StuI
StyI TatI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AleI AlfI
AloI AscI AseI AsiSI AvrII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI
BspHI BsrDI BsrGI BssHII BssSI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SnaBI SpeI SrfI SspI
SwaI TaqII Tth111I XbaI XcmI XhoI ZraI