(Linear) (Six-base) MAPSORT of: KIBB6595.seq  Check: 5837  from: 1  to: 1140



KIBB6595.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59    1140 
   Size:         59    1081
 
AgeI A'CCGG_T

Cuts at:      0    1075    1140 
   Size:       1075      65
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     338     370    1140 
   Size:        338      32     770

  Fragments arranged by size: 

                 770     338      32
 
ApoI r'AATT_y

Cuts at:      0     401     959    1123    1140 
   Size:        401     558     164      17

  Fragments arranged by size: 

                 558     401     164      17
 
AvaI C'yCGr_G

Cuts at:      0     740    1140 
   Size:        740     400
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     123     156    1140 
   Size:        123      33     984

  Fragments arranged by size: 

                 984     123      33
 
BamHI G'GATC_C

Cuts at:      0     123    1140 
   Size:        123    1017
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     250    1007    1140 
   Size:        250     757     133

  Fragments arranged by size: 

                 757     250     133
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     893     927    1140 
   Size:        893      34     213

  Fragments arranged by size: 

                 893     213      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     124    1140 
   Size:        124    1016
 
BfrBI ATG'CAT

Cuts at:      0     541    1140 
   Size:        541     599
 
BsaWI w'CCGG_w

Cuts at:      0    1075    1140 
   Size:       1075      65
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      92    1140 
   Size:         92    1048
 
BseYI C'CCAG_C

Cuts at:      0     989    1140 
   Size:        989     151
 
BsmI GAATG_Cn'

Cuts at:      0     196    1140 
   Size:        196     944
 
BsrDI GCAATG_nn'

Cuts at:      0     160     340    1140 
   Size:        160     180     800

  Fragments arranged by size: 

                 800     180     160
 
BsrFI r'CCGG_y

Cuts at:      0    1075    1140 
   Size:       1075      65
 
BstYI r'GATC_y

Cuts at:      0     123     670    1140 
   Size:        123     547     470

  Fragments arranged by size: 

                 547     470     123
 
Bsu36I CC'TnA_GG

Cuts at:      0     104    1140 
   Size:        104    1036
 
BtgI C'CryG_G

Cuts at:      0     328    1140 
   Size:        328     812
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59    1140 
   Size:         59    1081
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     479    1140 
   Size:        479     661
 
EcoO109I rG'GnC_Cy

Cuts at:      0     787    1140 
   Size:        787     353
 
EcoRI G'AATT_C

Cuts at:      0     401    1140 
   Size:        401     739
 
EcoRV GAT'ATC

Cuts at:      0     389    1140 
   Size:        389     751
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     108     140    1140 
   Size:        108      32    1000

  Fragments arranged by size: 

                1000     108      32
 
HincII GTy'rAC

Cuts at:      0      96    1140 
   Size:         96    1044
 
HpaI GTT'AAC

Cuts at:      0      96    1140 
   Size:         96    1044
 
MfeI C'AATT_G

Cuts at:      0     444    1140 
   Size:        444     696
 
MslI CAynn'nnrTG

Cuts at:      0     333     546    1140 
   Size:        333     213     594

  Fragments arranged by size: 

                 594     333     213
 
NcoI C'CATG_G

Cuts at:      0     328    1140 
   Size:        328     812
 
NsiI A_TGCA'T

Cuts at:      0     543    1140 
   Size:        543     597
 
PfoI T'CCnGG_A

Cuts at:      0     126    1140 
   Size:        126    1014
 
PpuMI rG'GwC_Cy

Cuts at:      0     787    1140 
   Size:        787     353
 
SfcI C'TryA_G

Cuts at:      0     857    1140 
   Size:        857     283
 
SspI AAT'ATT

Cuts at:      0     454     939    1058    1140 
   Size:        454     485     119      82

  Fragments arranged by size: 

                 485     454     119      82
 
StyI C'CwwG_G

Cuts at:      0     328     845    1140 
   Size:        328     517     295

  Fragments arranged by size: 

                 517     328     295
 
TatI w'GTAC_w

Cuts at:      0      64     486    1140 
   Size:         64     422     654

  Fragments arranged by size: 

                 654     422      64
 

 Enzymes that do cut:

     AcuI     AgeI     AloI     ApoI     AvaI     BaeI    BamHI     BbsI
     BcgI    BciVI    BfrBI    BsaWI    BseRI    BseYI     BsmI    BsrDI
    BsrFI    BstYI   Bsu36I     BtgI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV    Hin4I   HincII     HpaI     MfeI     MslI     NcoI     NsiI
     PfoI    PpuMI     SfcI     SspI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BanI    BanII     BbeI    BbvCI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI     FalI     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI