Restriction map for
pF1KB6583
(Linear) (Six-base) MAPSORT of: KIBB6583.seq Check: 1170 from: 1 to: 1131
KIBB6583.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1068 1131
Size: 1068 63
AatII G_ACGT'C
Cuts at: 0 914 1131
Size: 914 217
AleI CACnn'nnGTG
Cuts at: 0 94 253 1131
Size: 94 159 878
Fragments arranged by size:
878 159 94
AlwNI CAG_nnn'CTG
Cuts at: 0 525 1131
Size: 525 606
ApaI G_GGCC'C
Cuts at: 0 244 883 1131
Size: 244 639 248
Fragments arranged by size:
639 248 244
ApoI r'AATT_y
Cuts at: 0 111 1131
Size: 111 1020
AvaI C'yCGr_G
Cuts at: 0 71 116 847 1099 1131
Size: 71 45 731 252 32
Fragments arranged by size:
731 252 71 45 32
AvrII C'CTAG_G
Cuts at: 0 1026 1131
Size: 1026 105
BamHI G'GATC_C
Cuts at: 0 605 1131
Size: 605 526
BanI G'GyrC_C
Cuts at: 0 328 1105 1131
Size: 328 777 26
Fragments arranged by size:
777 328 26
BanII G_rGCy'C
Cuts at: 0 133 244 856 883 1131
Size: 133 111 612 27 248
Fragments arranged by size:
612 248 133 111 27
BbeI G_GCGC'C
Cuts at: 0 332 1109 1131
Size: 332 777 22
Fragments arranged by size:
777 332 22
BglI GCCn_nnn'nGGC
Cuts at: 0 851 1131
Size: 851 280
Bme1580I G_kGCm'C
Cuts at: 0 214 244 883 1131
Size: 214 30 639 248
Fragments arranged by size:
639 248 214 30
BmgBI CAC'GTC
Cuts at: 0 815 1131
Size: 815 316
BmrI ACTGGGnnnn_n'
Cuts at: 0 724 1131
Size: 724 407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 454 631 735 1131
Size: 32 422 177 104 396
Fragments arranged by size:
422 396 177 104 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1131
Size: 619 512
BsaI GGTCTCn'nnnn_
Cuts at: 0 457 1131
Size: 457 674
BsaHI Gr'CG_yC
Cuts at: 0 4 329 911 1106 1131
Size: 4 325 582 195 25
Fragments arranged by size:
582 325 195 25 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 961 964 991 1000 1030 1131
Size: 961 3 27 9 30 101
Fragments arranged by size:
961 101 30 27 9 3
BseYI C'CCAG_C
Cuts at: 0 243 876 1131
Size: 243 633 255
Fragments arranged by size:
633 255 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 1131
Size: 1054 77
BsiEI CG_ry'CG
Cuts at: 0 414 764 1131
Size: 414 350 367
Fragments arranged by size:
414 367 350
BsmI GAATG_Cn'
Cuts at: 0 151 1131
Size: 151 980
Bsp1286I G_dGCh'C
Cuts at: 0 133 214 244 856 883 1131
Size: 133 81 30 612 27 248
Fragments arranged by size:
612 248 133 81 30 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 1068 1131
Size: 398 670 63
Fragments arranged by size:
670 398 63
BsrBI CCG'CTC
Cuts at: 0 1125 1131
Size: 1125 6
BsrDI GCAATG_nn'
Cuts at: 0 353 1131
Size: 353 778
BsrFI r'CCGG_y
Cuts at: 0 448 1131
Size: 448 683
BssSI C'ACGA_G
Cuts at: 0 565 1131
Size: 565 566
BstAPI GCAn_nnn'nTGC
Cuts at: 0 355 1131
Size: 355 776
BstEII G'GTnAC_C
Cuts at: 0 861 1131
Size: 861 270
BstXI CCAn_nnnn'nTGG
Cuts at: 0 255 345 1131
Size: 255 90 786
Fragments arranged by size:
786 255 90
BstYI r'GATC_y
Cuts at: 0 548 605 710 725 1131
Size: 548 57 105 15 406
Fragments arranged by size:
548 406 105 57 15
BtgI C'CryG_G
Cuts at: 0 89 857 1131
Size: 89 768 274
Fragments arranged by size:
768 274 89
BtsI GCAGTG_nn'
Cuts at: 0 101 212 1131
Size: 101 111 919
Fragments arranged by size:
919 111 101
DraI TTT'AAA
Cuts at: 0 671 1131
Size: 671 460
EaeI y'GGCC_r
Cuts at: 0 335 411 572 761 1131
Size: 335 76 161 189 370
Fragments arranged by size:
370 335 189 161 76
EagI C'GGCC_G
Cuts at: 0 411 761 1131
Size: 411 350 370
Fragments arranged by size:
411 370 350
EarI CTCTTCn'nnn_
Cuts at: 0 176 949 1131
Size: 176 773 182
Fragments arranged by size:
773 182 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 1131
Size: 112 1019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 454 631 735 1131
Size: 32 422 177 104 396
Fragments arranged by size:
422 396 177 104 32
EcoO109I rG'GnC_Cy
Cuts at: 0 880 1131
Size: 880 251
EcoRI G'AATT_C
Cuts at: 0 111 1131
Size: 111 1020
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 682 1131
Size: 682 449
HaeII r_GCGC'y
Cuts at: 0 332 1109 1131
Size: 332 777 22
Fragments arranged by size:
777 332 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 474 506 799 831 1131
Size: 217 32 225 32 293 32 300
Fragments arranged by size:
300 293 225 217 32 32 32
KasI G'GCGC_C
Cuts at: 0 328 1105 1131
Size: 328 777 26
Fragments arranged by size:
777 328 26
MscI TGG'CCA
Cuts at: 0 337 1131
Size: 337 794
MslI CAynn'nnrTG
Cuts at: 0 94 253 343 1131
Size: 94 159 90 788
Fragments arranged by size:
788 159 94 90
MspA1I CmG'CkG
Cuts at: 0 81 432 577 1131
Size: 81 351 145 554
Fragments arranged by size:
554 351 145 81
NarI GG'CG_CC
Cuts at: 0 329 1106 1131
Size: 329 777 25
Fragments arranged by size:
777 329 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 602 876 1131
Size: 570 32 274 255
Fragments arranged by size:
570 274 255 32
PfoI T'CCnGG_A
Cuts at: 0 48 518 551 608 1131
Size: 48 470 33 57 523
Fragments arranged by size:
523 470 57 48 33
PspOMI G'GGCC_C
Cuts at: 0 240 879 1131
Size: 240 639 252
Fragments arranged by size:
639 252 240
PvuII CAG'CTG
Cuts at: 0 432 1131
Size: 432 699
SfcI C'TryA_G
Cuts at: 0 106 1131
Size: 106 1025
SfoI GGC'GCC
Cuts at: 0 330 1107 1131
Size: 330 777 24
Fragments arranged by size:
777 330 24
SmaI CCC'GGG
Cuts at: 0 118 1131
Size: 118 1013
SmlI C'TyrA_G
Cuts at: 0 71 634 1131
Size: 71 563 497
Fragments arranged by size:
563 497 71
StyI C'CwwG_G
Cuts at: 0 236 581 1026 1131
Size: 236 345 445 105
Fragments arranged by size:
445 345 236 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 606 1131
Size: 606 525
XhoI C'TCGA_G
Cuts at: 0 71 1131
Size: 71 1060
XmaI C'CCGG_G
Cuts at: 0 116 1131
Size: 116 1015
ZraI GAC'GTC
Cuts at: 0 912 1131
Size: 912 219
Enzymes that do cut:
AarI AatII AleI AlwNI ApaI ApoI AvaI AvrII
BamHI BanI BanII BbeI BglI Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII BstXI BstYI BtgI BtsI DraI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
KasI MscI MslI MspA1I NarI PflMI PfoI PspOMI
PvuII SfcI SfoI SmaI SmlI StyI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrGI BssHII BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EcoICRI EcoNI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XmnI