Restriction map for
pF1KB6581
(Linear) (Six-base) MAPSORT of: KIBB6581.seq Check: 9617 from: 1 to: 1131
KIBB6581.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AatII G_ACGT'C
Cuts at: 0 965 1131
Size: 965 166
AclI AA'CG_TT
Cuts at: 0 693 1131
Size: 693 438
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1131
Size: 214 917
AflII C'TTAA_G
Cuts at: 0 954 1131
Size: 954 177
ApaI G_GGCC'C
Cuts at: 0 387 1131
Size: 387 744
ApoI r'AATT_y
Cuts at: 0 499 751 931 970 1131
Size: 499 252 180 39 161
Fragments arranged by size:
499 252 180 161 39
AvaI C'yCGr_G
Cuts at: 0 116 305 380 1131
Size: 116 189 75 751
Fragments arranged by size:
751 189 116 75
BamHI G'GATC_C
Cuts at: 0 942 1131
Size: 942 189
BanII G_rGCy'C
Cuts at: 0 387 1131
Size: 387 744
BbsI GAAGACnn'nnnn_
Cuts at: 0 606 1131
Size: 606 525
BciVI GTATCCnnnnn_n'
Cuts at: 0 1083 1131
Size: 1083 48
BclI T'GATC_A
Cuts at: 0 275 1131
Size: 275 856
BglI GCCn_nnn'nGGC
Cuts at: 0 391 1131
Size: 391 740
Bme1580I G_kGCm'C
Cuts at: 0 387 1131
Size: 387 744
BmgBI CAC'GTC
Cuts at: 0 1083 1131
Size: 1083 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 517 1131
Size: 517 614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 907 1096 1131
Size: 729 178 189 35
Fragments arranged by size:
729 189 178 35
BsaBI GATnn'nnATC
Cuts at: 0 205 1131
Size: 205 926
BsaHI Gr'CG_yC
Cuts at: 0 492 962 1131
Size: 492 470 169
Fragments arranged by size:
492 470 169
BsaWI w'CCGG_w
Cuts at: 0 989 1131
Size: 989 142
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 904 1131
Size: 259 645 227
Fragments arranged by size:
645 259 227
BseYI C'CCAG_C
Cuts at: 0 159 1131
Size: 159 972
BsmI GAATG_Cn'
Cuts at: 0 483 1131
Size: 483 648
BsmBI CGTCTCn'nnnn_
Cuts at: 0 953 1131
Size: 953 178
Bsp1286I G_dGCh'C
Cuts at: 0 387 1131
Size: 387 744
BspEI T'CCGG_A
Cuts at: 0 989 1131
Size: 989 142
BsrBI CCG'CTC
Cuts at: 0 176 1131
Size: 176 955
BsrDI GCAATG_nn'
Cuts at: 0 852 1131
Size: 852 279
BsrFI r'CCGG_y
Cuts at: 0 488 1131
Size: 488 643
BssHII G'CGCG_C
Cuts at: 0 234 236 238 1131
Size: 234 2 2 893
Fragments arranged by size:
893 234 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1122 1131
Size: 1122 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 352 1131
Size: 352 779
BstYI r'GATC_y
Cuts at: 0 149 942 1131
Size: 149 793 189
Fragments arranged by size:
793 189 149
BtgI C'CryG_G
Cuts at: 0 213 389 1131
Size: 213 176 742
Fragments arranged by size:
742 213 176
BtsI GCAGTG_nn'
Cuts at: 0 948 1040 1131
Size: 948 92 91
Fragments arranged by size:
948 92 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 241 1054 1131
Size: 19 222 813 77
Fragments arranged by size:
813 222 77 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1131
Size: 214 917
EcoO109I rG'GnC_Cy
Cuts at: 0 384 1131
Size: 384 747
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 897 1131
Size: 204 693 234
Fragments arranged by size:
693 234 204
HindIII A'AGCT_T
Cuts at: 0 313 1131
Size: 313 818
MspA1I CmG'CkG
Cuts at: 0 467 1131
Size: 467 664
NcoI C'CATG_G
Cuts at: 0 389 1131
Size: 389 742
PasI CC'CwG_GG
Cuts at: 0 326 1131
Size: 326 805
PflMI CCAn_nnn'nTGG
Cuts at: 0 405 1131
Size: 405 726
PfoI T'CCnGG_A
Cuts at: 0 530 1131
Size: 530 601
PshAI GACnn'nnGTC
Cuts at: 0 491 1131
Size: 491 640
PspOMI G'GGCC_C
Cuts at: 0 383 1131
Size: 383 748
RsrII CG'GwC_CG
Cuts at: 0 23 1131
Size: 23 1108
SexAI A'CCwGG_T
Cuts at: 0 758 1131
Size: 758 373
SfcI C'TryA_G
Cuts at: 0 264 1126 1131
Size: 264 862 5
Fragments arranged by size:
862 264 5
SmaI CCC'GGG
Cuts at: 0 382 1131
Size: 382 749
SmlI C'TyrA_G
Cuts at: 0 305 744 886 954 1111 1131
Size: 305 439 142 68 157 20
Fragments arranged by size:
439 305 157 142 68 20
SspI AAT'ATT
Cuts at: 0 526 1131
Size: 526 605
StyI C'CwwG_G
Cuts at: 0 92 389 399 1131
Size: 92 297 10 732
Fragments arranged by size:
732 297 92 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 1131
Size: 499 632
XhoI C'TCGA_G
Cuts at: 0 305 1131
Size: 305 826
XmaI C'CCGG_G
Cuts at: 0 380 1131
Size: 380 751
ZraI GAC'GTC
Cuts at: 0 963 1131
Size: 963 168
Enzymes that do cut:
AatII AclI AcuI AflII ApaI ApoI AvaI BamHI
BanII BbsI BciVI BclI BglI Bme1580I BmgBI BmrI
BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsmI BsmBI
Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BstAPI BstXI
BstYI BtgI BtsI EciI Eco57MI EcoO109I FalI HindIII
MspA1I NcoI PasI PflMI PfoI PshAI PspOMI RsrII
SexAI SfcI SmaI SmlI SspI StyI TaqII XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BfrBI BglII BlpI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaXI BsgI
BsiEI BsiHKAI BsiWI BspHI BspMI BsrGI BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI TatI TstI Tth111I XbaI
XcmI XmnI