Restriction map for
pF1KB6564
(Linear) (Six-base) MAPSORT of: KIBB6564.seq Check: 7555 from: 1 to: 1200
KIBB6564.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 851 1200
Size: 851 349
Acc65I G'GTAC_C
Cuts at: 0 791 1200
Size: 791 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1200
Size: 1099 101
AleI CACnn'nnGTG
Cuts at: 0 1195 1200
Size: 1195 5
AlwNI CAG_nnn'CTG
Cuts at: 0 363 474 1200
Size: 363 111 726
Fragments arranged by size:
726 363 111
ApaI G_GGCC'C
Cuts at: 0 64 1200
Size: 64 1136
AvaI C'yCGr_G
Cuts at: 0 279 496 901 1200
Size: 279 217 405 299
Fragments arranged by size:
405 299 279 217
BanI G'GyrC_C
Cuts at: 0 82 791 905 1200
Size: 82 709 114 295
Fragments arranged by size:
709 295 114 82
BanII G_rGCy'C
Cuts at: 0 64 1200
Size: 64 1136
BbeI G_GCGC'C
Cuts at: 0 86 909 1200
Size: 86 823 291
Fragments arranged by size:
823 291 86
BglI GCCn_nnn'nGGC
Cuts at: 0 91 903 1200
Size: 91 812 297
Fragments arranged by size:
812 297 91
Bme1580I G_kGCm'C
Cuts at: 0 64 688 724 1200
Size: 64 624 36 476
Fragments arranged by size:
624 476 64 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 102 1200
Size: 102 1098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 771 844 1200
Size: 142 629 73 356
Fragments arranged by size:
629 356 142 73
Bpu10I CC'TnA_GC
Cuts at: 0 992 1200
Size: 992 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1200
Size: 334 866
BsaHI Gr'CG_yC
Cuts at: 0 83 812 906 1200
Size: 83 729 94 294
Fragments arranged by size:
729 294 94 83
BsaWI w'CCGG_w
Cuts at: 0 332 705 1200
Size: 332 373 495
Fragments arranged by size:
495 373 332
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 442 472 1200
Size: 442 30 728
Fragments arranged by size:
728 442 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 514 1200
Size: 139 375 686
Fragments arranged by size:
686 375 139
BseYI C'CCAG_C
Cuts at: 0 231 1059 1200
Size: 231 828 141
Fragments arranged by size:
828 231 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 757 1200
Size: 349 408 443
Fragments arranged by size:
443 408 349
BsiEI CG_ry'CG
Cuts at: 0 892 1200
Size: 892 308
BsmI GAATG_Cn'
Cuts at: 0 376 1200
Size: 376 824
Bsp1286I G_dGCh'C
Cuts at: 0 64 688 724 1200
Size: 64 624 36 476
Fragments arranged by size:
624 476 64 36
BspEI T'CCGG_A
Cuts at: 0 332 705 1200
Size: 332 373 495
Fragments arranged by size:
495 373 332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 348 1200
Size: 116 232 852
Fragments arranged by size:
852 232 116
BsrBI CCG'CTC
Cuts at: 0 647 711 1068 1200
Size: 647 64 357 132
Fragments arranged by size:
647 357 132 64
BsrFI r'CCGG_y
Cuts at: 0 18 27 324 839 935 1138 1200
Size: 18 9 297 515 96 203 62
Fragments arranged by size:
515 297 203 96 62 18 9
BssSI C'ACGA_G
Cuts at: 0 601 1200
Size: 601 599
BstAPI GCAn_nnn'nTGC
Cuts at: 0 363 1200
Size: 363 837
BstBI TT'CG_AA
Cuts at: 0 215 1200
Size: 215 985
BstEII G'GTnAC_C
Cuts at: 0 255 1200
Size: 255 945
BstXI CCAn_nnnn'nTGG
Cuts at: 0 822 1200
Size: 822 378
BstYI r'GATC_y
Cuts at: 0 221 503 1200
Size: 221 282 697
Fragments arranged by size:
697 282 221
BtgI C'CryG_G
Cuts at: 0 665 845 944 1200
Size: 665 180 99 256
Fragments arranged by size:
665 256 180 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 507 1200
Size: 36 471 693
Fragments arranged by size:
693 471 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 83 118 1200
Size: 83 35 1082
Fragments arranged by size:
1082 83 35
EaeI y'GGCC_r
Cuts at: 0 293 326 648 668 779 837 889 947
Size: 293 33 322 20 111 58 52 58
Cuts at: 947 1200
Size: 253
Fragments arranged by size:
322 293 253 111 58 58 52 33
20
EagI C'GGCC_G
Cuts at: 0 889 1200
Size: 889 311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1087 1200
Size: 1087 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 771 844 1099 1200
Size: 142 629 73 255 101
Fragments arranged by size:
629 255 142 101 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 1200
Size: 466 734
EcoO109I rG'GnC_Cy
Cuts at: 0 60 360 404 896 1200
Size: 60 300 44 492 304
Fragments arranged by size:
492 304 300 60 44
FseI GG_CCGG'CC
Cuts at: 0 843 1200
Size: 843 357
HaeII r_GCGC'y
Cuts at: 0 34 86 909 1200
Size: 34 52 823 291
Fragments arranged by size:
823 291 52 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 442 474 605 637 1200
Size: 49 32 361 32 131 32 563
Fragments arranged by size:
563 361 131 49 32 32 32
HincII GTy'rAC
Cuts at: 0 852 1200
Size: 852 348
KasI G'GCGC_C
Cuts at: 0 82 905 1200
Size: 82 823 295
Fragments arranged by size:
823 295 82
KpnI G_GTAC'C
Cuts at: 0 795 1200
Size: 795 405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 722 765 1200
Size: 617 105 43 435
Fragments arranged by size:
617 435 105 43
MscI TGG'CCA
Cuts at: 0 295 670 949 1200
Size: 295 375 279 251
Fragments arranged by size:
375 295 279 251
MslI CAynn'nnrTG
Cuts at: 0 436 578 601 1195 1200
Size: 436 142 23 594 5
Fragments arranged by size:
594 436 142 23 5
MspA1I CmG'CkG
Cuts at: 0 917 1063 1200
Size: 917 146 137
Fragments arranged by size:
917 146 137
NaeI GCC'GGC
Cuts at: 0 29 326 841 937 1140 1200
Size: 29 297 515 96 203 60
Fragments arranged by size:
515 297 203 96 60 29
NarI GG'CG_CC
Cuts at: 0 83 906 1200
Size: 83 823 294
Fragments arranged by size:
823 294 83
NcoI C'CATG_G
Cuts at: 0 665 944 1200
Size: 665 279 256
Fragments arranged by size:
665 279 256
NgoMIV G'CCGG_C
Cuts at: 0 27 324 839 935 1138 1200
Size: 27 297 515 96 203 62
Fragments arranged by size:
515 297 203 96 62 27
PfoI T'CCnGG_A
Cuts at: 0 962 1025 1200
Size: 962 63 175
Fragments arranged by size:
962 175 63
PpuMI rG'GwC_Cy
Cuts at: 0 360 1200
Size: 360 840
PspOMI G'GGCC_C
Cuts at: 0 60 1200
Size: 60 1140
PstI C_TGCA'G
Cuts at: 0 113 359 1200
Size: 113 246 841
Fragments arranged by size:
841 246 113
RsrII CG'GwC_CG
Cuts at: 0 1112 1200
Size: 1112 88
SalI G'TCGA_C
Cuts at: 0 850 1200
Size: 850 350
SexAI A'CCwGG_T
Cuts at: 0 98 467 1200
Size: 98 369 733
Fragments arranged by size:
733 369 98
SfcI C'TryA_G
Cuts at: 0 109 355 411 1200
Size: 109 246 56 789
Fragments arranged by size:
789 246 109 56
SfoI GGC'GCC
Cuts at: 0 84 907 1200
Size: 84 823 293
Fragments arranged by size:
823 293 84
SmaI CCC'GGG
Cuts at: 0 903 1200
Size: 903 297
SmlI C'TyrA_G
Cuts at: 0 313 1200
Size: 313 887
StyI C'CwwG_G
Cuts at: 0 185 665 944 1200
Size: 185 480 279 256
Fragments arranged by size:
480 279 256 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 1197 1200
Size: 190 1007 3
Fragments arranged by size:
1007 190 3
XbaI T'CTAG_A
Cuts at: 0 610 1200
Size: 610 590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 678 1200
Size: 678 522
XmaI C'CCGG_G
Cuts at: 0 901 1200
Size: 901 299
XmnI GAAnn'nnTTC
Cuts at: 0 167 1200
Size: 167 1033
Enzymes that do cut:
AccI Acc65I AcuI AleI AlwNI ApaI AvaI BanI
BanII BbeI BglI Bme1580I BplI BpmI Bpu10I BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI CspCI EaeI EagI
EciI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PfoI PpuMI PspOMI PstI RsrII SalI
SexAI SfcI SfoI SmaI SmlI StyI TaqII XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI
BsiHKAI BsiWI BsmBI BspHI BsrDI BsrGI BssHII BstZ17I
Bsu36I BtsI ClaI DraI DraIII DrdI EarI EcoICRI
EcoRI EcoRV FalI FspI FspAI HindIII HpaI MfeI
MluI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII SacI SacII SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XhoI
ZraI