Restriction map for
pF1KB6559
(Linear) (Six-base) MAPSORT of: KIBB6559.seq Check: 3660 from: 1 to: 1119
KIBB6559.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 646 1119
Size: 646 473
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 440 1119
Size: 371 69 679
Fragments arranged by size:
679 371 69
AflIII A'CryG_T
Cuts at: 0 802 1119
Size: 802 317
AhdI GACnn_n'nnGTC
Cuts at: 0 457 1119
Size: 457 662
AleI CACnn'nnGTG
Cuts at: 0 805 1119
Size: 805 314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 629 1119
Size: 629 490
AlwNI CAG_nnn'CTG
Cuts at: 0 185 517 914 1119
Size: 185 332 397 205
Fragments arranged by size:
397 332 205 185
ApaI G_GGCC'C
Cuts at: 0 771 1119
Size: 771 348
ApoI r'AATT_y
Cuts at: 0 892 1119
Size: 892 227
AvaI C'yCGr_G
Cuts at: 0 154 575 935 1119
Size: 154 421 360 184
Fragments arranged by size:
421 360 184 154
BamHI G'GATC_C
Cuts at: 0 1016 1119
Size: 1016 103
BanI G'GyrC_C
Cuts at: 0 51 1119
Size: 51 1068
BanII G_rGCy'C
Cuts at: 0 771 1037 1119
Size: 771 266 82
Fragments arranged by size:
771 266 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 1119
Size: 154 965
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 674 708 1119
Size: 674 34 411
Fragments arranged by size:
674 411 34
BfrBI ATG'CAT
Cuts at: 0 968 1119
Size: 968 151
Bme1580I G_kGCm'C
Cuts at: 0 771 1119
Size: 771 348
BmgBI CAC'GTC
Cuts at: 0 288 1119
Size: 288 831
BmrI ACTGGGnnnn_n'
Cuts at: 0 612 1119
Size: 612 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 739 1119
Size: 317 422 380
Fragments arranged by size:
422 380 317
BsaAI yAC'GTr
Cuts at: 0 629 803 1119
Size: 629 174 316
Fragments arranged by size:
629 316 174
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 254 298 370 1119
Size: 254 44 72 749
Fragments arranged by size:
749 254 72 44
BseYI C'CCAG_C
Cuts at: 0 178 441 655 1119
Size: 178 263 214 464
Fragments arranged by size:
464 263 214 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 391 1119
Size: 209 182 728
Fragments arranged by size:
728 209 182
BsmI GAATG_Cn'
Cuts at: 0 970 1119
Size: 970 149
Bsp1286I G_dGCh'C
Cuts at: 0 771 1037 1119
Size: 771 266 82
Fragments arranged by size:
771 266 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 646 820 1119
Size: 646 174 299
Fragments arranged by size:
646 299 174
BsrDI GCAATG_nn'
Cuts at: 0 386 1119
Size: 386 733
BsrFI r'CCGG_y
Cuts at: 0 219 1119
Size: 219 900
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1085 1119
Size: 1085 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 655 679 1119
Size: 655 24 440
Fragments arranged by size:
655 440 24
BstYI r'GATC_y
Cuts at: 0 1016 1119
Size: 1016 103
Bsu36I CC'TnA_GG
Cuts at: 0 475 1119
Size: 475 644
BtgI C'CryG_G
Cuts at: 0 74 482 1037 1119
Size: 74 408 555 82
Fragments arranged by size:
555 408 82 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 80 1119
Size: 80 1039
EaeI y'GGCC_r
Cuts at: 0 71 117 1119
Size: 71 46 1002
Fragments arranged by size:
1002 71 46
EarI CTCTTCn'nnn_
Cuts at: 0 13 244 347 485 595 1078 1119
Size: 13 231 103 138 110 483 41
Fragments arranged by size:
483 231 138 110 103 41 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 1119
Size: 94 1025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 371 440 739 1119
Size: 317 54 69 299 380
Fragments arranged by size:
380 317 299 69 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 775 1119
Size: 775 344
EcoO109I rG'GnC_Cy
Cuts at: 0 377 438 451 767 768 1119
Size: 377 61 13 316 1 351
Fragments arranged by size:
377 351 316 61 13 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 443 475 1119
Size: 443 32 644
Fragments arranged by size:
644 443 32
MslI CAynn'nnrTG
Cuts at: 0 805 1119
Size: 805 314
MspA1I CmG'CkG
Cuts at: 0 405 445 651 1119
Size: 405 40 206 468
Fragments arranged by size:
468 405 206 40
NaeI GCC'GGC
Cuts at: 0 221 1119
Size: 221 898
NcoI C'CATG_G
Cuts at: 0 482 1119
Size: 482 637
NgoMIV G'CCGG_C
Cuts at: 0 219 1119
Size: 219 900
NsiI A_TGCA'T
Cuts at: 0 970 1119
Size: 970 149
PasI CC'CwG_GG
Cuts at: 0 125 771 846 1119
Size: 125 646 75 273
Fragments arranged by size:
646 273 125 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 185 1119
Size: 185 934
PfoI T'CCnGG_A
Cuts at: 0 272 749 1119
Size: 272 477 370
Fragments arranged by size:
477 370 272
PmlI CAC'GTG
Cuts at: 0 629 803 1119
Size: 629 174 316
Fragments arranged by size:
629 316 174
PpuMI rG'GwC_Cy
Cuts at: 0 451 1119
Size: 451 668
PspOMI G'GGCC_C
Cuts at: 0 767 1119
Size: 767 352
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 850 882 1119
Size: 850 32 237
Fragments arranged by size:
850 237 32
PstI C_TGCA'G
Cuts at: 0 210 910 1015 1119
Size: 210 700 105 104
Fragments arranged by size:
700 210 105 104
PvuII CAG'CTG
Cuts at: 0 405 651 1119
Size: 405 246 468
Fragments arranged by size:
468 405 246
SapI GCTCTTCn'nnn_
Cuts at: 0 244 485 1119
Size: 244 241 634
Fragments arranged by size:
634 244 241
SfcI C'TryA_G
Cuts at: 0 206 885 906 996 1011 1119
Size: 206 679 21 90 15 108
Fragments arranged by size:
679 206 108 90 21 15
StuI AGG'CCT
Cuts at: 0 781 1119
Size: 781 338
StyI C'CwwG_G
Cuts at: 0 482 1044 1119
Size: 482 562 75
Fragments arranged by size:
562 482 75
TatI w'GTAC_w
Cuts at: 0 866 1119
Size: 866 253
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbsI BcgI BfrBI
Bme1580I BmgBI BmrI BpmI BsaAI BseRI BseYI BsgI
BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI EaeI EarI EciI Eco57MI EcoNI
EcoO109I Hin4I MslI MspA1I NaeI NcoI NgoMIV NsiI
PasI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI
PvuII SapI SfcI StuI StyI TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BciVI BclI BglI BglII BlpI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NdeI NheI NotI NruI
NspI PacI PciI PmeI PpiI PshAI PsiI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI