Restriction map for
pF1KB6557
(Linear) (Six-base) MAPSORT of: KIBB6557.seq Check: 8876 from: 1 to: 1116
KIBB6557.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 296 1116
Size: 296 820
AccI GT'mk_AC
Cuts at: 0 59 1116
Size: 59 1057
AhdI GACnn_n'nnGTC
Cuts at: 0 705 1116
Size: 705 411
AleI CACnn'nnGTG
Cuts at: 0 601 1116
Size: 601 515
BanI G'GyrC_C
Cuts at: 0 87 284 978 1041 1116
Size: 87 197 694 63 75
Fragments arranged by size:
694 197 87 75 63
BanII G_rGCy'C
Cuts at: 0 118 1116
Size: 118 998
BglI GCCn_nnn'nGGC
Cuts at: 0 251 1116
Size: 251 865
BglII A'GATC_T
Cuts at: 0 998 1116
Size: 998 118
BlpI GC'TnA_GC
Cuts at: 0 340 1116
Size: 340 776
Bme1580I G_kGCm'C
Cuts at: 0 647 1116
Size: 647 469
BmgBI CAC'GTC
Cuts at: 0 123 167 674 1116
Size: 123 44 507 442
Fragments arranged by size:
507 442 123 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 159 250 1116
Size: 159 91 866
Fragments arranged by size:
866 159 91
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 685 897 1116
Size: 602 83 212 219
Fragments arranged by size:
602 219 212 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 703 1116
Size: 463 240 413
Fragments arranged by size:
463 413 240
BsaI GGTCTCn'nnnn_
Cuts at: 0 540 943 1116
Size: 540 403 173
Fragments arranged by size:
540 403 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 262 292 499 520 529 550 658 688
Size: 262 30 207 21 9 21 108 30
Cuts at: 688 1116
Size: 428
Fragments arranged by size:
428 262 207 108 30 30 21 21
9
BseYI C'CCAG_C
Cuts at: 0 471 646 1017 1116
Size: 471 175 371 99
Fragments arranged by size:
471 371 175 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1116
Size: 118 998
BsiHKAI G_wGCw'C
Cuts at: 0 227 341 1116
Size: 227 114 775
Fragments arranged by size:
775 227 114
Bsp1286I G_dGCh'C
Cuts at: 0 118 227 341 647 1116
Size: 118 109 114 306 469
Fragments arranged by size:
469 306 118 114 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 296 1116
Size: 209 87 820
Fragments arranged by size:
820 209 87
BsrBI CCG'CTC
Cuts at: 0 350 1116
Size: 350 766
BsrDI GCAATG_nn'
Cuts at: 0 558 1116
Size: 558 558
BsrFI r'CCGG_y
Cuts at: 0 659 1116
Size: 659 457
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 975 1071 1116
Size: 453 522 96 45
Fragments arranged by size:
522 453 96 45
BstYI r'GATC_y
Cuts at: 0 998 1116
Size: 998 118
BtgI C'CryG_G
Cuts at: 0 16 228 325 929 1116
Size: 16 212 97 604 187
Fragments arranged by size:
604 212 187 97 16
BtsI GCAGTG_nn'
Cuts at: 0 127 1094 1116
Size: 127 967 22
Fragments arranged by size:
967 127 22
EaeI y'GGCC_r
Cuts at: 0 637 1116
Size: 637 479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 685 897 1116
Size: 602 83 212 219
Fragments arranged by size:
602 219 212 83
EcoO109I rG'GnC_Cy
Cuts at: 0 468 1033 1116
Size: 468 565 83
Fragments arranged by size:
565 468 83
HaeII r_GCGC'y
Cuts at: 0 111 1116
Size: 111 1005
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 691 723 731 763 1116
Size: 60 32 599 32 8 32 353
Fragments arranged by size:
599 353 60 32 32 32 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 803 1116
Size: 377 426 313
Fragments arranged by size:
426 377 313
MslI CAynn'nnrTG
Cuts at: 0 132 601 1116
Size: 132 469 515
Fragments arranged by size:
515 469 132
MspA1I CmG'CkG
Cuts at: 0 219 327 344 383 488 1025 1087 1116
Size: 219 108 17 39 105 537 62 29
Fragments arranged by size:
537 219 108 105 62 39 29 17
NaeI GCC'GGC
Cuts at: 0 661 1116
Size: 661 455
NcoI C'CATG_G
Cuts at: 0 228 1116
Size: 228 888
NgoMIV G'CCGG_C
Cuts at: 0 659 1116
Size: 659 457
NspI r_CATG'y
Cuts at: 0 313 507 769 1116
Size: 313 194 262 347
Fragments arranged by size:
347 313 262 194
PasI CC'CwG_GG
Cuts at: 0 558 1009 1116
Size: 558 451 107
Fragments arranged by size:
558 451 107
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 1116
Size: 234 882
PfoI T'CCnGG_A
Cuts at: 0 749 1116
Size: 749 367
PpuMI rG'GwC_Cy
Cuts at: 0 468 1033 1116
Size: 468 565 83
Fragments arranged by size:
565 468 83
PstI C_TGCA'G
Cuts at: 0 382 1116
Size: 382 734
PvuII CAG'CTG
Cuts at: 0 219 344 383 1116
Size: 219 125 39 733
Fragments arranged by size:
733 219 125 39
SacII CC_GC'GG
Cuts at: 0 328 1116
Size: 328 788
SanDI GG'GwC_CC
Cuts at: 0 1033 1116
Size: 1033 83
SfcI C'TryA_G
Cuts at: 0 378 1116
Size: 378 738
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 251 1116
Size: 251 865
SmlI C'TyrA_G
Cuts at: 0 478 718 1116
Size: 478 240 398
Fragments arranged by size:
478 398 240
StyI C'CwwG_G
Cuts at: 0 228 365 845 1116
Size: 228 137 480 271
Fragments arranged by size:
480 271 228 137
TatI w'GTAC_w
Cuts at: 0 527 824 1116
Size: 527 297 292
Fragments arranged by size:
527 297 292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 262 294 393 425 499 531 658 690
Size: 262 32 99 32 74 32 127 32
Cuts at: 690 855 887 1116
Size: 165 32 229
Fragments arranged by size:
262 229 165 127 99 74 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 74 1116
Size: 74 1042
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 972 1116
Size: 972 144
Enzymes that do cut:
AarI AccI AhdI AleI BanI BanII BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaXI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstXI BstYI BtgI BtsI EaeI Eco57MI EcoO109I HaeII
Hin4I MmeI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PstI PvuII SacII SanDI
SfcI SfiI SmlI StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XbaI
XhoI XmaI XmnI ZraI