Restriction map for
pF1KB6553
(Linear) (Six-base) MAPSORT of: KIBB6553.seq Check: 6931 from: 1 to: 1113
KIBB6553.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AflIII A'CryG_T
Cuts at: 0 222 782 1113
Size: 222 560 331
Fragments arranged by size:
560 331 222
AlwNI CAG_nnn'CTG
Cuts at: 0 793 1113
Size: 793 320
AvaI C'yCGr_G
Cuts at: 0 92 110 1028 1113
Size: 92 18 918 85
Fragments arranged by size:
918 92 85 18
BanI G'GyrC_C
Cuts at: 0 213 645 708 737 852 897 912 1113
Size: 213 432 63 29 115 45 15 201
Fragments arranged by size:
432 213 201 115 63 45 29 15
BanII G_rGCy'C
Cuts at: 0 462 596 780 1113
Size: 462 134 184 333
Fragments arranged by size:
462 333 184 134
BbeI G_GCGC'C
Cuts at: 0 741 916 1113
Size: 741 175 197
Fragments arranged by size:
741 197 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 1113
Size: 219 894
BfrBI ATG'CAT
Cuts at: 0 976 1113
Size: 976 137
BglI GCCn_nnn'nGGC
Cuts at: 0 203 256 298 735 758 1113
Size: 203 53 42 437 23 355
Fragments arranged by size:
437 355 203 53 42 23
Bme1580I G_kGCm'C
Cuts at: 0 216 855 1029 1113
Size: 216 639 174 84
Fragments arranged by size:
639 216 174 84
BmgBI CAC'GTC
Cuts at: 0 225 1113
Size: 225 888
BmrI ACTGGGnnnn_n'
Cuts at: 0 219 1113
Size: 219 894
BmtI G_CTAG'C
Cuts at: 0 109 1113
Size: 109 1004
Bpu10I CC'TnA_GC
Cuts at: 0 157 1113
Size: 157 956
BsaAI yAC'GTr
Cuts at: 0 783 1113
Size: 783 330
BsaHI Gr'CG_yC
Cuts at: 0 10 738 750 913 1113
Size: 10 728 12 163 200
Fragments arranged by size:
728 200 163 12 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 767 797 1113
Size: 767 30 316
Fragments arranged by size:
767 316 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 366 1077 1113
Size: 366 711 36
Fragments arranged by size:
711 366 36
BseYI C'CCAG_C
Cuts at: 0 49 58 284 518 536 575 848 1045
Size: 49 9 226 234 18 39 273 197
Cuts at: 1045 1113
Size: 68
Fragments arranged by size:
273 234 226 197 68 49 39 18
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 939 1056 1113
Size: 889 50 117 57
Fragments arranged by size:
889 117 57 50
BsiEI CG_ry'CG
Cuts at: 0 309 475 1113
Size: 309 166 638
Fragments arranged by size:
638 309 166
BsiHKAI G_wGCw'C
Cuts at: 0 680 1113
Size: 680 433
Bsp1286I G_dGCh'C
Cuts at: 0 216 462 596 680 780 855 1029 1113
Size: 216 246 134 84 100 75 174 84
Fragments arranged by size:
246 216 174 134 100 84 84 75
BsrDI GCAATG_nn'
Cuts at: 0 168 1113
Size: 168 945
BsrFI r'CCGG_y
Cuts at: 0 197 470 474 1113
Size: 197 273 4 639
Fragments arranged by size:
639 273 197 4
BstXI CCAn_nnnn'nTGG
Cuts at: 0 402 454 1113
Size: 402 52 659
Fragments arranged by size:
659 402 52
BstYI r'GATC_y
Cuts at: 0 968 1113
Size: 968 145
BtgI C'CryG_G
Cuts at: 0 260 315 447 1113
Size: 260 55 132 666
Fragments arranged by size:
666 260 132 55
EaeI y'GGCC_r
Cuts at: 0 234 248 257 392 472 797 938 1094
Size: 234 14 9 135 80 325 141 156
Cuts at: 1094 1113
Size: 19
Fragments arranged by size:
325 234 156 141 135 80 19 14
9
EagI C'GGCC_G
Cuts at: 0 472 1113
Size: 472 641
EarI CTCTTCn'nnn_
Cuts at: 0 529 1113
Size: 529 584
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 130 1113
Size: 130 983
EcoNI CCTnn'n_nnAGG
Cuts at: 0 905 1096 1113
Size: 905 191 17
Fragments arranged by size:
905 191 17
EcoO109I rG'GnC_Cy
Cuts at: 0 96 888 1054 1113
Size: 96 792 166 59
Fragments arranged by size:
792 166 96 59
HaeII r_GCGC'y
Cuts at: 0 127 741 916 1113
Size: 127 614 175 197
Fragments arranged by size:
614 197 175 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1093 1113
Size: 1093 20
HindIII A'AGCT_T
Cuts at: 0 1011 1113
Size: 1011 102
KasI G'GCGC_C
Cuts at: 0 737 912 1113
Size: 737 175 201
Fragments arranged by size:
737 201 175
MscI TGG'CCA
Cuts at: 0 236 394 799 940 1096 1113
Size: 236 158 405 141 156 17
Fragments arranged by size:
405 236 158 156 141 17
MslI CAynn'nnrTG
Cuts at: 0 452 1113
Size: 452 661
MspA1I CmG'CkG
Cuts at: 0 25 102 288 560 793 873 953 1113
Size: 25 77 186 272 233 80 80 160
Fragments arranged by size:
272 233 186 160 80 80 77 25
NaeI GCC'GGC
Cuts at: 0 199 1113
Size: 199 914
NarI GG'CG_CC
Cuts at: 0 738 913 1113
Size: 738 175 200
Fragments arranged by size:
738 200 175
NcoI C'CATG_G
Cuts at: 0 447 1113
Size: 447 666
NgoMIV G'CCGG_C
Cuts at: 0 197 1113
Size: 197 916
NheI G'CTAG_C
Cuts at: 0 105 1113
Size: 105 1008
NsiI A_TGCA'T
Cuts at: 0 978 1113
Size: 978 135
NspI r_CATG'y
Cuts at: 0 369 1113
Size: 369 744
PasI CC'CwG_GG
Cuts at: 0 596 654 1113
Size: 596 58 459
Fragments arranged by size:
596 459 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 965 1113
Size: 965 148
PfoI T'CCnGG_A
Cuts at: 0 686 1113
Size: 686 427
PmlI CAC'GTG
Cuts at: 0 783 1113
Size: 783 330
PpuMI rG'GwC_Cy
Cuts at: 0 96 888 1054 1113
Size: 96 792 166 59
Fragments arranged by size:
792 166 96 59
PstI C_TGCA'G
Cuts at: 0 356 1113
Size: 356 757
PvuII CAG'CTG
Cuts at: 0 288 793 873 953 1113
Size: 288 505 80 80 160
Fragments arranged by size:
505 288 160 80 80
SanDI GG'GwC_CC
Cuts at: 0 96 888 1054 1113
Size: 96 792 166 59
Fragments arranged by size:
792 166 96 59
SapI GCTCTTCn'nnn_
Cuts at: 0 529 1113
Size: 529 584
SfcI C'TryA_G
Cuts at: 0 352 1113
Size: 352 761
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 203 256 298 1113
Size: 203 53 42 815
Fragments arranged by size:
815 203 53 42
SfoI GGC'GCC
Cuts at: 0 739 914 1113
Size: 739 175 199
Fragments arranged by size:
739 199 175
SmaI CCC'GGG
Cuts at: 0 94 112 1030 1113
Size: 94 18 918 83
Fragments arranged by size:
918 94 83 18
SrfI GCCC'GGGC
Cuts at: 0 112 1113
Size: 112 1001
StuI AGG'CCT
Cuts at: 0 729 1113
Size: 729 384
StyI C'CwwG_G
Cuts at: 0 447 1097 1113
Size: 447 650 16
Fragments arranged by size:
650 447 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 1059 1113
Size: 231 828 54
Fragments arranged by size:
828 231 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 767 799 845 877 1113
Size: 767 32 46 32 236
Fragments arranged by size:
767 236 46 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 1089 1113
Size: 141 948 24
Fragments arranged by size:
948 141 24
XmaI C'CCGG_G
Cuts at: 0 92 110 1028 1113
Size: 92 18 918 85
Fragments arranged by size:
918 92 85 18
Enzymes that do cut:
AflIII AlwNI AvaI BanI BanII BbeI BbsI BfrBI
BglI Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrDI
BsrFI BstXI BstYI BtgI EaeI EagI EarI EciI
EcoNI EcoO109I HaeII Hin4I HindIII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PflMI PfoI PmlI PpuMI PstI PvuII SanDI
SapI SfcI SfiI SfoI SmaI SrfI StuI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BglII BlpI BplI BpmI BpuEI BsaI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI NdeI NotI
NruI PacI PciI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI