Restriction map for
pF1KB6548
(Linear) (Six-base) MAPSORT of: KIBB6548.seq Check: 3117 from: 1 to: 1176
KIBB6548.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
Acc65I G'GTAC_C
Cuts at: 0 406 1176
Size: 406 770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 577 808 1079 1176
Size: 22 555 231 271 97
Fragments arranged by size:
555 271 231 97 22
AflII C'TTAA_G
Cuts at: 0 982 1176
Size: 982 194
AflIII A'CryG_T
Cuts at: 0 111 767 1176
Size: 111 656 409
Fragments arranged by size:
656 409 111
AlwNI CAG_nnn'CTG
Cuts at: 0 129 240 424 555 945 1176
Size: 129 111 184 131 390 231
Fragments arranged by size:
390 231 184 131 129 111
ApaI G_GGCC'C
Cuts at: 0 875 1054 1176
Size: 875 179 122
Fragments arranged by size:
875 179 122
AvaI C'yCGr_G
Cuts at: 0 175 729 1176
Size: 175 554 447
Fragments arranged by size:
554 447 175
BanI G'GyrC_C
Cuts at: 0 406 1176
Size: 406 770
BanII G_rGCy'C
Cuts at: 0 434 722 875 965 1054 1176
Size: 434 288 153 90 89 122
Fragments arranged by size:
434 288 153 122 90 89
BbvCI CC'TCA_GC
Cuts at: 0 572 886 1176
Size: 572 314 290
Fragments arranged by size:
572 314 290
BglI GCCn_nnn'nGGC
Cuts at: 0 373 560 1176
Size: 373 187 616
Fragments arranged by size:
616 373 187
BlpI GC'TnA_GC
Cuts at: 0 959 1007 1176
Size: 959 48 169
Fragments arranged by size:
959 169 48
Bme1580I G_kGCm'C
Cuts at: 0 74 875 1054 1176
Size: 74 801 179 122
Fragments arranged by size:
801 179 122 74
BmgBI CAC'GTC
Cuts at: 0 768 1176
Size: 768 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 1176
Size: 12 1164
Bpu10I CC'TnA_GC
Cuts at: 0 572 886 1176
Size: 572 314 290
Fragments arranged by size:
572 314 290
BsaI GGTCTCn'nnnn_
Cuts at: 0 204 1176
Size: 204 972
BsaAI yAC'GTr
Cuts at: 0 114 1176
Size: 114 1062
BsaBI GATnn'nnATC
Cuts at: 0 1117 1176
Size: 1117 59
BsaHI Gr'CG_yC
Cuts at: 0 899 1176
Size: 899 277
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 754 784 1176
Size: 754 30 392
Fragments arranged by size:
754 392 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 336 526 601 604 607 712 1176
Size: 238 98 190 75 3 3 105 464
Fragments arranged by size:
464 238 190 105 98 75 3 3
BseYI C'CCAG_C
Cuts at: 0 74 880 977 1069 1165 1176
Size: 74 806 97 92 96 11
Fragments arranged by size:
806 97 96 92 74 11
BsiEI CG_ry'CG
Cuts at: 0 854 1176
Size: 854 322
BsiHKAI G_wGCw'C
Cuts at: 0 20 1176
Size: 20 1156
BsmI GAATG_Cn'
Cuts at: 0 48 1176
Size: 48 1128
Bsp1286I G_dGCh'C
Cuts at: 0 20 74 434 722 875 965 1054 1176
Size: 20 54 360 288 153 90 89 122
Fragments arranged by size:
360 288 153 122 90 89 54 20
BsrFI r'CCGG_y
Cuts at: 0 849 1011 1176
Size: 849 162 165
Fragments arranged by size:
849 165 162
BstEII G'GTnAC_C
Cuts at: 0 135 1176
Size: 135 1041
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 1176
Size: 828 348
BstYI r'GATC_y
Cuts at: 0 125 341 412 1094 1176
Size: 125 216 71 682 82
Fragments arranged by size:
682 216 125 82 71
BtgI C'CryG_G
Cuts at: 0 776 1103 1176
Size: 776 327 73
Fragments arranged by size:
776 327 73
EaeI y'GGCC_r
Cuts at: 0 161 653 851 1176
Size: 161 492 198 325
Fragments arranged by size:
492 325 198 161
EagI C'GGCC_G
Cuts at: 0 851 1176
Size: 851 325
EarI CTCTTCn'nnn_
Cuts at: 0 485 527 920 1176
Size: 485 42 393 256
Fragments arranged by size:
485 393 256 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 530 1176
Size: 530 646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 22 577 808 1079 1176
Size: 12 10 555 231 271 97
Fragments arranged by size:
555 271 231 97 12 10
EcoO109I rG'GnC_Cy
Cuts at: 0 288 753 871 1176
Size: 288 465 118 305
Fragments arranged by size:
465 305 288 118
HaeII r_GCGC'y
Cuts at: 0 601 1176
Size: 601 575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 757 786 789 1176
Size: 754 3 29 3 387
Fragments arranged by size:
754 387 29 3 3
KpnI G_GTAC'C
Cuts at: 0 410 1176
Size: 410 766
MspA1I CmG'CkG
Cuts at: 0 1033 1176
Size: 1033 143
NaeI GCC'GGC
Cuts at: 0 851 1176
Size: 851 325
NcoI C'CATG_G
Cuts at: 0 776 1103 1176
Size: 776 327 73
Fragments arranged by size:
776 327 73
NgoMIV G'CCGG_C
Cuts at: 0 849 1176
Size: 849 327
PasI CC'CwG_GG
Cuts at: 0 418 828 1176
Size: 418 410 348
Fragments arranged by size:
418 410 348
PflMI CCAn_nnn'nTGG
Cuts at: 0 129 195 1104 1176
Size: 129 66 909 72
Fragments arranged by size:
909 129 72 66
PfoI T'CCnGG_A
Cuts at: 0 314 455 1133 1176
Size: 314 141 678 43
Fragments arranged by size:
678 314 141 43
PmlI CAC'GTG
Cuts at: 0 114 1176
Size: 114 1062
PpuMI rG'GwC_Cy
Cuts at: 0 288 753 1176
Size: 288 465 423
Fragments arranged by size:
465 423 288
PspOMI G'GGCC_C
Cuts at: 0 871 1050 1176
Size: 871 179 126
Fragments arranged by size:
871 179 126
PstI C_TGCA'G
Cuts at: 0 429 1176
Size: 429 747
PvuII CAG'CTG
Cuts at: 0 1033 1176
Size: 1033 143
SfcI C'TryA_G
Cuts at: 0 425 1176
Size: 425 751
SmaI CCC'GGG
Cuts at: 0 731 1176
Size: 731 445
SmlI C'TyrA_G
Cuts at: 0 982 1176
Size: 982 194
SrfI GCCC'GGGC
Cuts at: 0 731 1176
Size: 731 445
StyI C'CwwG_G
Cuts at: 0 359 581 776 905 1103 1176
Size: 359 222 195 129 198 73
Fragments arranged by size:
359 222 198 195 129 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 820 894 1176
Size: 820 74 282
Fragments arranged by size:
820 282 74
TatI w'GTAC_w
Cuts at: 0 209 1127 1176
Size: 209 918 49
Fragments arranged by size:
918 209 49
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 996 1110 1176
Size: 996 114 66
Fragments arranged by size:
996 114 66
XmaI C'CCGG_G
Cuts at: 0 729 1176
Size: 729 447
Enzymes that do cut:
Acc65I AcuI AflII AflIII AlwNI ApaI AvaI BanI
BanII BbvCI BglI BlpI Bme1580I BmgBI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BsrFI BstEII BstXI BstYI BtgI
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I
KpnI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SfcI SmaI SmlI
SrfI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglII BmrI BmtI BplI BpuEI BsaWI BsgI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MmeI MscI MslI NarI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI