(Linear) (Six-base) MAPSORT of: KIBB6546.seq  Check: 5761  from: 1  to: 1176



KIBB6546.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     426    1176 
   Size:        426     750
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     631     697    1176 
   Size:        520     111      66     479

  Fragments arranged by size: 

                 520     479     111      66
 
AflII C'TTAA_G

Cuts at:      0     154    1176 
   Size:        154    1022
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     832    1176 
   Size:        832     344
 
ApoI r'AATT_y

Cuts at:      0     447     828    1023    1176 
   Size:        447     381     195     153

  Fragments arranged by size: 

                 447     381     195     153
 
BamHI G'GATC_C

Cuts at:      0     588    1176 
   Size:        588     588
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     143     608     797    1040    1176 
   Size:        143     465     189     243     136

  Fragments arranged by size: 

                 465     243     189     143     136
 
BbvCI CC'TCA_GC

Cuts at:      0     412    1176 
   Size:        412     764
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     278     312    1176 
   Size:        278      34     864

  Fragments arranged by size: 

                 864     278      34
 
BfrBI ATG'CAT

Cuts at:      0     283     664    1176 
   Size:        283     381     512

  Fragments arranged by size: 

                 512     381     283
 
BglII A'GATC_T

Cuts at:      0     621    1176 
   Size:        621     555
 
Bme1580I G_kGCm'C

Cuts at:      0     774    1176 
   Size:        774     402
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     480     678    1176 
   Size:        480     198     498

  Fragments arranged by size: 

                 498     480     198
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     391    1176 
   Size:         56     335     785

  Fragments arranged by size: 

                 785     335      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     412    1176 
   Size:        412     764
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     695    1176 
   Size:        695     481
 
BsaBI GATnn'nnATC

Cuts at:      0     593    1176 
   Size:        593     583
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      54      84    1176 
   Size:         54      30    1092

  Fragments arranged by size: 

                1092      54      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     394    1176 
   Size:        394     782
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802    1176 
   Size:        802     374
 
Bsp1286I G_dGCh'C

Cuts at:      0     774    1176 
   Size:        774     402
 
BspHI T'CATG_A

Cuts at:      0      39    1176 
   Size:         39    1137
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     426    1176 
   Size:        426     750
 
BstYI r'GATC_y

Cuts at:      0     503     588     621     970    1176 
   Size:        503      85      33     349     206

  Fragments arranged by size: 

                 503     349     206      85      33
 
BtgI C'CryG_G

Cuts at:      0     248     507    1176 
   Size:        248     259     669

  Fragments arranged by size: 

                 669     259     248
 
BtsI GCAGTG_nn'

Cuts at:      0     125     792    1129    1176 
   Size:        125     667     337      47

  Fragments arranged by size: 

                 667     337     125      47
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     230     265    1176 
   Size:        230      35     911

  Fragments arranged by size: 

                 911     230      35
 
DraI TTT'AAA

Cuts at:      0     452    1176 
   Size:        452     724
 
EarI CTCTTCn'nnn_

Cuts at:      0     184     313     607     673     795    1176 
   Size:        184     129     294      66     122     381

  Fragments arranged by size: 

                 381     294     184     129     122      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     391     520     631     697    1176 
   Size:         56     335     129     111      66     479

  Fragments arranged by size: 

                 479     335     129     111      66      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     908    1176 
   Size:        908     268
 
EcoRV GAT'ATC

Cuts at:      0      24    1176 
   Size:         24    1152
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     606     638    1176 
   Size:        606      32     538

  Fragments arranged by size: 

                 606     538      32
 
MspA1I CmG'CkG

Cuts at:      0     411    1176 
   Size:        411     765
 
NcoI C'CATG_G

Cuts at:      0     507    1176 
   Size:        507     669
 
NdeI CA'TA_TG

Cuts at:      0     132    1176 
   Size:        132    1044
 
NsiI A_TGCA'T

Cuts at:      0     285     666    1176 
   Size:        285     381     510

  Fragments arranged by size: 

                 510     381     285
 
NspI r_CATG'y

Cuts at:      0     664     995    1176 
   Size:        664     331     181

  Fragments arranged by size: 

                 664     331     181
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     513    1176 
   Size:        513     663
 
PsiI TTA'TAA

Cuts at:      0    1169    1176 
   Size:       1169       7
 
PvuII CAG'CTG

Cuts at:      0     411    1176 
   Size:        411     765
 
SapI GCTCTTCn'nnn_

Cuts at:      0     313     607    1176 
   Size:        313     294     569

  Fragments arranged by size: 

                 569     313     294
 
SfcI C'TryA_G

Cuts at:      0     568     776    1176 
   Size:        568     208     400

  Fragments arranged by size: 

                 568     400     208
 
SmlI C'TyrA_G

Cuts at:      0     154     710    1176 
   Size:        154     556     466

  Fragments arranged by size: 

                 556     466     154
 
SphI G_CATG'C

Cuts at:      0     664    1176 
   Size:        664     512
 
StuI AGG'CCT

Cuts at:      0     274    1176 
   Size:        274     902
 
StyI C'CwwG_G

Cuts at:      0     507     795    1176 
   Size:        507     288     381

  Fragments arranged by size: 

                 507     381     288
 
SwaI ATTT'AAAT

Cuts at:      0     452    1176 
   Size:        452     724
 
TatI w'GTAC_w

Cuts at:      0     714    1176 
   Size:        714     462
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     837     869    1176 
   Size:        837      32     307

  Fragments arranged by size: 

                 837     307      32
 

 Enzymes that do cut:

     AarI     AcuI    AflII     AlfI     ApoI    BamHI     BbsI    BbvCI
     BcgI    BfrBI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
    BsaBI    BsaXI    BseRI     BsgI Bsp1286I    BspHI    BspMI    BstYI
     BtgI     BtsI    CspCI     DraI     EarI  Eco57MI    EcoNI    EcoRV
    Hin4I   MspA1I     NcoI     NdeI     NsiI     NspI    PflMI     PsiI
    PvuII     SapI     SfcI     SmlI     SphI     StuI     StyI     SwaI
     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BciVI     BclI
     BglI     BlpI    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI