Restriction map for
pF1KB6535
(Linear) (Six-base) MAPSORT of: KIBB6535.seq Check: 6125 from: 1 to: 1101
KIBB6535.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 271 1101
Size: 271 830
AccI GT'mk_AC
Cuts at: 0 383 1101
Size: 383 718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1101
Size: 334 767
AleI CACnn'nnGTG
Cuts at: 0 286 475 811 1024 1101
Size: 286 189 336 213 77
Fragments arranged by size:
336 286 213 189 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 753 1042 1101
Size: 753 289 59
Fragments arranged by size:
753 289 59
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 546 1101
Size: 514 32 555
Fragments arranged by size:
555 514 32
AlwNI CAG_nnn'CTG
Cuts at: 0 109 610 1101
Size: 109 501 491
Fragments arranged by size:
501 491 109
ApaLI G'TGCA_C
Cuts at: 0 481 1101
Size: 481 620
ApoI r'AATT_y
Cuts at: 0 551 1101
Size: 551 550
AvaI C'yCGr_G
Cuts at: 0 557 1101
Size: 557 544
BanI G'GyrC_C
Cuts at: 0 787 1101
Size: 787 314
BanII G_rGCy'C
Cuts at: 0 564 769 1101
Size: 564 205 332
Fragments arranged by size:
564 332 205
BbvCI CC'TCA_GC
Cuts at: 0 209 1101
Size: 209 892
BciVI GTATCCnnnnn_n'
Cuts at: 0 157 1101
Size: 157 944
BglI GCCn_nnn'nGGC
Cuts at: 0 643 1101
Size: 643 458
BlpI GC'TnA_GC
Cuts at: 0 32 563 1101
Size: 32 531 538
Fragments arranged by size:
538 531 32
Bme1580I G_kGCm'C
Cuts at: 0 485 575 1101
Size: 485 90 526
Fragments arranged by size:
526 485 90
BmgBI CAC'GTC
Cuts at: 0 423 1101
Size: 423 678
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 851 896 1101
Size: 565 286 45 205
Fragments arranged by size:
565 286 205 45
Bpu10I CC'TnA_GC
Cuts at: 0 209 1101
Size: 209 892
BsaI GGTCTCn'nnnn_
Cuts at: 0 975 1101
Size: 975 126
BsaWI w'CCGG_w
Cuts at: 0 220 1101
Size: 220 881
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 845 875 1101
Size: 514 30 301 30 226
Fragments arranged by size:
514 301 226 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 899 1101
Size: 899 202
BseYI C'CCAG_C
Cuts at: 0 1051 1101
Size: 1051 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1101
Size: 1048 53
BsiHKAI G_wGCw'C
Cuts at: 0 485 769 1101
Size: 485 284 332
Fragments arranged by size:
485 332 284
Bsp1286I G_dGCh'C
Cuts at: 0 485 564 575 769 1101
Size: 485 79 11 194 332
Fragments arranged by size:
485 332 194 79 11
BspEI T'CCGG_A
Cuts at: 0 220 1101
Size: 220 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 271 668 745 953 1101
Size: 271 397 77 208 148
Fragments arranged by size:
397 271 208 148 77
BstAPI GCAn_nnn'nTGC
Cuts at: 0 434 727 1101
Size: 434 293 374
Fragments arranged by size:
434 374 293
BstEII G'GTnAC_C
Cuts at: 0 980 1101
Size: 980 121
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 813 1101
Size: 288 525 288
Fragments arranged by size:
525 288 288
BstZ17I GTA'TAC
Cuts at: 0 384 1101
Size: 384 717
Bsu36I CC'TnA_GG
Cuts at: 0 987 1101
Size: 987 114
BtgI C'CryG_G
Cuts at: 0 377 812 1101
Size: 377 435 289
Fragments arranged by size:
435 377 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 110 1101
Size: 110 991
BtsI GCAGTG_nn'
Cuts at: 0 737 1101
Size: 737 364
DraIII CAC_nnn'GTG
Cuts at: 0 703 1101
Size: 703 398
EaeI y'GGCC_r
Cuts at: 0 503 1101
Size: 503 598
EcoICRI GAG'CTC
Cuts at: 0 767 1101
Size: 767 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 565 851 896 1101
Size: 334 231 286 45 205
Fragments arranged by size:
334 286 231 205 45
FspI TGC'GCA
Cuts at: 0 427 1101
Size: 427 674
HindIII A'AGCT_T
Cuts at: 0 709 1101
Size: 709 392
MslI CAynn'nnrTG
Cuts at: 0 286 475 811 958 964 1024 1101
Size: 286 189 336 147 6 60 77
Fragments arranged by size:
336 286 189 147 77 60 6
MspA1I CmG'CkG
Cuts at: 0 312 351 462 543 871 1101
Size: 312 39 111 81 328 230
Fragments arranged by size:
328 312 230 111 81 39
NspI r_CATG'y
Cuts at: 0 325 1101
Size: 325 776
PasI CC'CwG_GG
Cuts at: 0 67 1101
Size: 67 1034
PflMI CCAn_nnn'nTGG
Cuts at: 0 273 1101
Size: 273 828
PfoI T'CCnGG_A
Cuts at: 0 912 1101
Size: 912 189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 1101
Size: 514 32 555
Fragments arranged by size:
555 514 32
PvuII CAG'CTG
Cuts at: 0 312 351 543 871 1101
Size: 312 39 192 328 230
Fragments arranged by size:
328 312 230 192 39
SacI G_AGCT'C
Cuts at: 0 769 1101
Size: 769 332
SfcI C'TryA_G
Cuts at: 0 131 1101
Size: 131 970
SmaI CCC'GGG
Cuts at: 0 559 1101
Size: 559 542
SphI G_CATG'C
Cuts at: 0 325 1101
Size: 325 776
StyI C'CwwG_G
Cuts at: 0 157 1101
Size: 157 944
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 952 1101
Size: 952 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 845 877 1101
Size: 845 32 224
Fragments arranged by size:
845 224 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 540 813 1101
Size: 540 273 288
Fragments arranged by size:
540 288 273
XmaI C'CCGG_G
Cuts at: 0 557 1101
Size: 557 544
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AloI AlwNI ApaLI
ApoI AvaI BanI BanII BbvCI BciVI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI
EcoICRI Eco57MI FspI HindIII MslI MspA1I NspI PasI
PflMI PfoI PpiI PvuII SacI SfcI SmaI SphI
StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BclI BfrBI BglII BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstYI ClaI CspCI DraI DrdI EagI EarI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmnI ZraI