Restriction map for
pF1KB6528
(Linear) (Six-base) MAPSORT of: KIBB6528.seq Check: 7992 from: 1 to: 1164
KIBB6528.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 431 1164
Size: 431 733
AflIII A'CryG_T
Cuts at: 0 472 513 1164
Size: 472 41 651
Fragments arranged by size:
651 472 41
AleI CACnn'nnGTG
Cuts at: 0 514 1164
Size: 514 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1160 1164
Size: 1160 4
AlwNI CAG_nnn'CTG
Cuts at: 0 342 1164
Size: 342 822
ApoI r'AATT_y
Cuts at: 0 589 985 1164
Size: 589 396 179
Fragments arranged by size:
589 396 179
AvaI C'yCGr_G
Cuts at: 0 349 1164
Size: 349 815
BanI G'GyrC_C
Cuts at: 0 412 491 1164
Size: 412 79 673
Fragments arranged by size:
673 412 79
BanII G_rGCy'C
Cuts at: 0 89 1164
Size: 89 1075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 936 970 1164
Size: 936 34 194
Fragments arranged by size:
936 194 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 593 1164
Size: 593 571
BclI T'GATC_A
Cuts at: 0 1007 1164
Size: 1007 157
BglI GCCn_nnn'nGGC
Cuts at: 0 22 1164
Size: 22 1142
Bme1580I G_kGCm'C
Cuts at: 0 349 496 1164
Size: 349 147 668
Fragments arranged by size:
668 349 147
BmgBI CAC'GTC
Cuts at: 0 473 1164
Size: 473 691
BmrI ACTGGGnnnn_n'
Cuts at: 0 783 1164
Size: 783 381
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 306 1164
Size: 306 858
Bpu10I CC'TnA_GC
Cuts at: 0 278 1164
Size: 278 886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1164
Size: 625 539
BsaI GGTCTCn'nnnn_
Cuts at: 0 896 1164
Size: 896 268
BsaAI yAC'GTr
Cuts at: 0 516 1164
Size: 516 648
BsaBI GATnn'nnATC
Cuts at: 0 1012 1164
Size: 1012 152
BsaWI w'CCGG_w
Cuts at: 0 242 1164
Size: 242 922
BsiEI CG_ry'CG
Cuts at: 0 1049 1164
Size: 1049 115
BsiHKAI G_wGCw'C
Cuts at: 0 65 857 1164
Size: 65 792 307
Fragments arranged by size:
792 307 65
Bsp1286I G_dGCh'C
Cuts at: 0 65 89 349 496 857 1164
Size: 65 24 260 147 361 307
Fragments arranged by size:
361 307 260 147 65 24
BspEI T'CCGG_A
Cuts at: 0 242 1164
Size: 242 922
BspHI T'CATG_A
Cuts at: 0 89 302 1164
Size: 89 213 862
Fragments arranged by size:
862 213 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 431 534 1164
Size: 431 103 630
Fragments arranged by size:
630 431 103
BsrFI r'CCGG_y
Cuts at: 0 400 409 1164
Size: 400 9 755
Fragments arranged by size:
755 400 9
BstYI r'GATC_y
Cuts at: 0 245 669 1164
Size: 245 424 495
Fragments arranged by size:
495 424 245
BtgI C'CryG_G
Cuts at: 0 176 740 1164
Size: 176 564 424
Fragments arranged by size:
564 424 176
BtsI GCAGTG_nn'
Cuts at: 0 1030 1164
Size: 1030 134
DraI TTT'AAA
Cuts at: 0 536 1164
Size: 536 628
DraIII CAC_nnn'GTG
Cuts at: 0 374 1164
Size: 374 790
DrdI GACnn_nn'nnGTC
Cuts at: 0 178 1066 1164
Size: 178 888 98
Fragments arranged by size:
888 178 98
EaeI y'GGCC_r
Cuts at: 0 737 1164
Size: 737 427
EarI CTCTTCn'nnn_
Cuts at: 0 301 1164
Size: 301 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 274 817 1164
Size: 274 543 347
Fragments arranged by size:
543 347 274
EcoO109I rG'GnC_Cy
Cuts at: 0 339 1164
Size: 339 825
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 230 261 262 293 1164
Size: 230 31 1 31 871
Fragments arranged by size:
871 230 31 31 1
HincII GTy'rAC
Cuts at: 0 771 1164
Size: 771 393
MslI CAynn'nnrTG
Cuts at: 0 514 739 964 1164
Size: 514 225 225 200
Fragments arranged by size:
514 225 225 200
MspA1I CmG'CkG
Cuts at: 0 392 1164
Size: 392 772
NaeI GCC'GGC
Cuts at: 0 402 411 1164
Size: 402 9 753
Fragments arranged by size:
753 402 9
NgoMIV G'CCGG_C
Cuts at: 0 400 409 1164
Size: 400 9 755
Fragments arranged by size:
755 400 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 735 1164
Size: 735 429
PfoI T'CCnGG_A
Cuts at: 0 728 1164
Size: 728 436
PmlI CAC'GTG
Cuts at: 0 516 1164
Size: 516 648
PshAI GACnn'nnGTC
Cuts at: 0 199 1164
Size: 199 965
PstI C_TGCA'G
Cuts at: 0 546 1164
Size: 546 618
PvuII CAG'CTG
Cuts at: 0 392 1164
Size: 392 772
ScaI AGT'ACT
Cuts at: 0 895 1164
Size: 895 269
SfcI C'TryA_G
Cuts at: 0 542 897 1164
Size: 542 355 267
Fragments arranged by size:
542 355 267
SmlI C'TyrA_G
Cuts at: 0 640 1164
Size: 640 524
StuI AGG'CCT
Cuts at: 0 940 1164
Size: 940 224
StyI C'CwwG_G
Cuts at: 0 197 1164
Size: 197 967
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 126 430 1164
Size: 126 304 734
Fragments arranged by size:
734 304 126
TatI w'GTAC_w
Cuts at: 0 394 893 1164
Size: 394 499 271
Fragments arranged by size:
499 394 271
Enzymes that do cut:
AarI AflIII AleI AlfI AlwNI ApoI AvaI BanI
BanII BcgI BciVI BclI BglI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrFI BstYI BtgI
BtsI DraI DraIII DrdI EaeI EarI EciI EcoO109I
Hin4I HincII MslI MspA1I NaeI NgoMIV PflMI PfoI
PmlI PshAI PstI PvuII ScaI SfcI SmlI StuI
StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BfrBI BglII BlpI
BmtI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiWI
BsmI BsmBI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
EagI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI