Restriction map for
pF1KB6525
(Linear) (Six-base) MAPSORT of: KIBB6525.seq Check: 5108 from: 1 to: 1095
KIBB6525.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1095
Size: 640 455
AleI CACnn'nnGTG
Cuts at: 0 379 1095
Size: 379 716
AlwNI CAG_nnn'CTG
Cuts at: 0 174 345 940 1095
Size: 174 171 595 155
Fragments arranged by size:
595 174 171 155
ApaI G_GGCC'C
Cuts at: 0 163 367 1095
Size: 163 204 728
Fragments arranged by size:
728 204 163
ApoI r'AATT_y
Cuts at: 0 423 1095
Size: 423 672
AvaI C'yCGr_G
Cuts at: 0 123 463 1095
Size: 123 340 632
Fragments arranged by size:
632 340 123
BamHI G'GATC_C
Cuts at: 0 763 1095
Size: 763 332
BanI G'GyrC_C
Cuts at: 0 381 442 727 1095
Size: 381 61 285 368
Fragments arranged by size:
381 368 285 61
BanII G_rGCy'C
Cuts at: 0 38 163 209 367 524 605 954 1095
Size: 38 125 46 158 157 81 349 141
Fragments arranged by size:
349 158 157 141 125 81 46 38
BbvCI CC'TCA_GC
Cuts at: 0 17 1095
Size: 17 1078
BglI GCCn_nnn'nGGC
Cuts at: 0 194 350 1095
Size: 194 156 745
Fragments arranged by size:
745 194 156
BglII A'GATC_T
Cuts at: 0 267 1095
Size: 267 828
Bme1580I G_kGCm'C
Cuts at: 0 163 367 386 445 1095
Size: 163 204 19 59 650
Fragments arranged by size:
650 204 163 59 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 370 1095
Size: 370 725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 1095
Size: 624 471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 610 860 1095
Size: 589 21 250 235
Fragments arranged by size:
589 250 235 21
Bpu10I CC'TnA_GC
Cuts at: 0 17 175 1095
Size: 17 158 920
Fragments arranged by size:
920 158 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1095
Size: 418 677
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 1095
Size: 253 842
BsaWI w'CCGG_w
Cuts at: 0 684 1095
Size: 684 411
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 206 236 1095
Size: 206 30 859
Fragments arranged by size:
859 206 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 962 987 1095
Size: 427 535 25 108
Fragments arranged by size:
535 427 108 25
BseYI C'CCAG_C
Cuts at: 0 30 156 348 631 1095
Size: 30 126 192 283 464
Fragments arranged by size:
464 283 192 126 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1095
Size: 850 245
BsiHKAI G_wGCw'C
Cuts at: 0 524 566 812 896 954 1095
Size: 524 42 246 84 58 141
Fragments arranged by size:
524 246 141 84 58 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1004 1095
Size: 1004 91
Bsp1286I G_dGCh'C
Cuts at: 0 38 163 209 367 386 445 524 566
Size: 38 125 46 158 19 59 79 42
Cuts at: 566 605 812 896 954 1095
Size: 39 207 84 58 141
Fragments arranged by size:
207 158 141 125 84 79 59 58
46 42 39 38 19
BspEI T'CCGG_A
Cuts at: 0 684 1095
Size: 684 411
BspHI T'CATG_A
Cuts at: 0 419 1095
Size: 419 676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 667 1095
Size: 667 428
BsrFI r'CCGG_y
Cuts at: 0 730 1095
Size: 730 365
BssSI C'ACGA_G
Cuts at: 0 895 1095
Size: 895 200
BstAPI GCAn_nnn'nTGC
Cuts at: 0 345 555 1095
Size: 345 210 540
Fragments arranged by size:
540 345 210
BstXI CCAn_nnnn'nTGG
Cuts at: 0 992 1095
Size: 992 103
BstYI r'GATC_y
Cuts at: 0 267 763 995 1095
Size: 267 496 232 100
Fragments arranged by size:
496 267 232 100
Bsu36I CC'TnA_GG
Cuts at: 0 856 1095
Size: 856 239
BtgI C'CryG_G
Cuts at: 0 10 104 579 1095
Size: 10 94 475 516
Fragments arranged by size:
516 475 94 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 818 1095
Size: 818 277
DrdI GACnn_nn'nnGTC
Cuts at: 0 1021 1095
Size: 1021 74
EaeI y'GGCC_r
Cuts at: 0 509 719 732 1095
Size: 509 210 13 363
Fragments arranged by size:
509 363 210 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 217 1095
Size: 217 878
EcoICRI GAG'CTC
Cuts at: 0 522 952 1095
Size: 522 430 143
Fragments arranged by size:
522 430 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 610 640 860 1095
Size: 589 21 30 220 235
Fragments arranged by size:
589 235 220 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 436 1095
Size: 436 659
EcoO109I rG'GnC_Cy
Cuts at: 0 6 325 342 364 468 1023 1095
Size: 6 319 17 22 104 555 72
Fragments arranged by size:
555 319 104 72 22 17 6
HaeII r_GCGC'y
Cuts at: 0 31 668 1095
Size: 31 637 427
Fragments arranged by size:
637 427 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 252 284 361 393 856 888
Size: 206 32 14 32 77 32 463 32
Cuts at: 888 1095
Size: 207
Fragments arranged by size:
463 207 206 77 32 32 32 32
14
HindIII A'AGCT_T
Cuts at: 0 299 308 1095
Size: 299 9 787
Fragments arranged by size:
787 299 9
MslI CAynn'nnrTG
Cuts at: 0 379 1095
Size: 379 716
MspA1I CmG'CkG
Cuts at: 0 12 106 486 581 724 937 1095
Size: 12 94 380 95 143 213 158
Fragments arranged by size:
380 213 158 143 95 94 12
PasI CC'CwG_GG
Cuts at: 0 23 1039 1095
Size: 23 1016 56
Fragments arranged by size:
1016 56 23
PpuMI rG'GwC_Cy
Cuts at: 0 6 325 1023 1095
Size: 6 319 698 72
Fragments arranged by size:
698 319 72 6
PspOMI G'GGCC_C
Cuts at: 0 159 363 1095
Size: 159 204 732
Fragments arranged by size:
732 204 159
PstI C_TGCA'G
Cuts at: 0 341 664 1095
Size: 341 323 431
Fragments arranged by size:
431 341 323
SacI G_AGCT'C
Cuts at: 0 524 954 1095
Size: 524 430 141
Fragments arranged by size:
524 430 141
SacII CC_GC'GG
Cuts at: 0 13 107 582 1095
Size: 13 94 475 513
Fragments arranged by size:
513 475 94 13
SanDI GG'GwC_CC
Cuts at: 0 6 1095
Size: 6 1089
SexAI A'CCwGG_T
Cuts at: 0 233 1095
Size: 233 862
SfcI C'TryA_G
Cuts at: 0 337 660 941 1095
Size: 337 323 281 154
Fragments arranged by size:
337 323 281 154
SmaI CCC'GGG
Cuts at: 0 125 1095
Size: 125 970
SmlI C'TyrA_G
Cuts at: 0 433 1095
Size: 433 662
StyI C'CwwG_G
Cuts at: 0 991 1095
Size: 991 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 132 1095
Size: 132 963
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 960 992 1095
Size: 960 32 103
Fragments arranged by size:
960 103 32
XmaI C'CCGG_G
Cuts at: 0 123 1095
Size: 123 972
Enzymes that do cut:
AcuI AleI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbvCI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI DrdI EaeI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
MslI MspA1I PasI PpuMI PspOMI PstI SacI SacII
SanDI SexAI SfcI SmaI SmlI StyI TaqII TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I
BtsI ClaI CspCI DraI DraIII EagI EarI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
XhoI XmnI ZraI