Restriction map for
pF1KB6523
(Linear) (Six-base) MAPSORT of: KIBB6523.seq Check: 5195 from: 1 to: 1092
KIBB6523.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 0 1040 1092
Size: 1040 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1092
Size: 698 394
AflIII A'CryG_T
Cuts at: 0 924 1092
Size: 924 168
AleI CACnn'nnGTG
Cuts at: 0 550 1092
Size: 550 542
ApaI G_GGCC'C
Cuts at: 0 176 1092
Size: 176 916
ApaLI G'TGCA_C
Cuts at: 0 928 1092
Size: 928 164
ApoI r'AATT_y
Cuts at: 0 271 1092
Size: 271 821
BanII G_rGCy'C
Cuts at: 0 137 176 1092
Size: 137 39 916
Fragments arranged by size:
916 137 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 1092
Size: 709 383
BclI T'GATC_A
Cuts at: 0 554 1092
Size: 554 538
BglII A'GATC_T
Cuts at: 0 498 1092
Size: 498 594
Bme1580I G_kGCm'C
Cuts at: 0 176 321 932 1092
Size: 176 145 611 160
Fragments arranged by size:
611 176 160 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 557 1092
Size: 557 535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 802 938 1092
Size: 40 762 136 154
Fragments arranged by size:
762 154 136 40
Bpu10I CC'TnA_GC
Cuts at: 0 29 245 1092
Size: 29 216 847
Fragments arranged by size:
847 216 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1092
Size: 967 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 619 649 911 941 1092
Size: 619 30 262 30 151
Fragments arranged by size:
619 262 151 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 232 235 238 637 640 1092
Size: 229 3 3 3 399 3 452
Fragments arranged by size:
452 399 229 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 42 163 1092
Size: 42 121 929
Fragments arranged by size:
929 121 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 1092
Size: 1024 68
BsiEI CG_ry'CG
Cuts at: 0 1050 1092
Size: 1050 42
BsiHKAI G_wGCw'C
Cuts at: 0 932 1092
Size: 932 160
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1007 1092
Size: 1007 85
Bsp1286I G_dGCh'C
Cuts at: 0 137 176 321 932 1092
Size: 137 39 145 611 160
Fragments arranged by size:
611 160 145 137 39
BsrDI GCAATG_nn'
Cuts at: 0 525 1092
Size: 525 567
BsrFI r'CCGG_y
Cuts at: 0 1045 1092
Size: 1045 47
BstXI CCAn_nnnn'nTGG
Cuts at: 0 145 1092
Size: 145 947
BstYI r'GATC_y
Cuts at: 0 428 498 943 1092
Size: 428 70 445 149
Fragments arranged by size:
445 428 149 70
BtsI GCAGTG_nn'
Cuts at: 0 161 1092
Size: 161 931
DraIII CAC_nnn'GTG
Cuts at: 0 936 1092
Size: 936 156
EaeI y'GGCC_r
Cuts at: 0 638 1047 1092
Size: 638 409 45
Fragments arranged by size:
638 409 45
EagI C'GGCC_G
Cuts at: 0 1047 1092
Size: 1047 45
EarI CTCTTCn'nnn_
Cuts at: 0 191 197 206 1092
Size: 191 6 9 886
Fragments arranged by size:
886 191 9 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 698 802 938 1092
Size: 40 658 104 136 154
Fragments arranged by size:
658 154 136 104 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 784 1092
Size: 784 308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 54 1092
Size: 54 1038
FspI TGC'GCA
Cuts at: 0 544 1092
Size: 544 548
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 803 835 1092
Size: 619 32 152 32 257
Fragments arranged by size:
619 257 152 32 32
HincII GTy'rAC
Cuts at: 0 255 1092
Size: 255 837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 858 1092
Size: 858 234
MslI CAynn'nnrTG
Cuts at: 0 550 1092
Size: 550 542
MspA1I CmG'CkG
Cuts at: 0 72 1092
Size: 72 1020
PflMI CCAn_nnn'nTGG
Cuts at: 0 999 1092
Size: 999 93
PspOMI G'GGCC_C
Cuts at: 0 172 1092
Size: 172 920
SexAI A'CCwGG_T
Cuts at: 0 435 1092
Size: 435 657
SfcI C'TryA_G
Cuts at: 0 67 101 453 468 605 1092
Size: 67 34 352 15 137 487
Fragments arranged by size:
487 352 137 67 34 15
SmlI C'TyrA_G
Cuts at: 0 982 1092
Size: 982 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 379 411 425 457 911 943 1092
Size: 379 32 14 32 454 32 149
Fragments arranged by size:
454 379 149 32 32 32 14
XbaI T'CTAG_A
Cuts at: 0 1080 1092
Size: 1080 12
Enzymes that do cut:
AccI AcuI AflIII AleI ApaI ApaLI ApoI BanII
BbsI BclI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BsrDI BsrFI BstXI BstYI BtsI DraIII EaeI EagI
EarI Eco57MI EcoNI FalI FspI Hin4I HincII MmeI
MslI MspA1I PflMI PspOMI SexAI SfcI SmlI TstI
XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI
BsaHI BsaWI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EciI EcoICRI
EcoO109I EcoRI EcoRV FseI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI Tth111I XcmI XhoI
XmaI XmnI ZraI