Restriction map for
pF1KB6502
(Linear) (Six-base) MAPSORT of: KIBB6502.seq Check: 4619 from: 1 to: 1143
KIBB6502.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 1115 1143
Size: 1115 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1143
Size: 390 753
AflIII A'CryG_T
Cuts at: 0 677 1143
Size: 677 466
AhdI GACnn_n'nnGTC
Cuts at: 0 540 1143
Size: 540 603
AleI CACnn'nnGTG
Cuts at: 0 283 1143
Size: 283 860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 1143
Size: 202 941
AlwNI CAG_nnn'CTG
Cuts at: 0 311 904 1020 1083 1143
Size: 311 593 116 63 60
Fragments arranged by size:
593 311 116 63 60
ApoI r'AATT_y
Cuts at: 0 688 1143
Size: 688 455
AvaI C'yCGr_G
Cuts at: 0 174 343 499 845 1143
Size: 174 169 156 346 298
Fragments arranged by size:
346 298 174 169 156
BanI G'GyrC_C
Cuts at: 0 191 271 1143
Size: 191 80 872
Fragments arranged by size:
872 191 80
BanII G_rGCy'C
Cuts at: 0 500 1143
Size: 500 643
BbeI G_GCGC'C
Cuts at: 0 195 1143
Size: 195 948
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 560 594 1143
Size: 560 34 549
Fragments arranged by size:
560 549 34
BglII A'GATC_T
Cuts at: 0 626 1143
Size: 626 517
Bme1580I G_kGCm'C
Cuts at: 0 26 1143
Size: 26 1117
BmgBI CAC'GTC
Cuts at: 0 678 1143
Size: 678 465
BmrI ACTGGGnnnn_n'
Cuts at: 0 613 801 1143
Size: 613 188 342
Fragments arranged by size:
613 342 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 810 1143
Size: 100 710 333
Fragments arranged by size:
710 333 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 349 1143
Size: 340 9 794
Fragments arranged by size:
794 340 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 436 551 1143
Size: 436 115 592
Fragments arranged by size:
592 436 115
BsaHI Gr'CG_yC
Cuts at: 0 192 1143
Size: 192 951
BseYI C'CCAG_C
Cuts at: 0 84 492 1038 1143
Size: 84 408 546 105
Fragments arranged by size:
546 408 105 84
BsiEI CG_ry'CG
Cuts at: 0 121 1143
Size: 121 1022
BsiHKAI G_wGCw'C
Cuts at: 0 290 1143
Size: 290 853
Bsp1286I G_dGCh'C
Cuts at: 0 26 290 500 1143
Size: 26 264 210 643
Fragments arranged by size:
643 264 210 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 1143
Size: 218 925
BsrBI CCG'CTC
Cuts at: 0 96 896 1143
Size: 96 800 247
Fragments arranged by size:
800 247 96
BsrDI GCAATG_nn'
Cuts at: 0 435 873 1143
Size: 435 438 270
Fragments arranged by size:
438 435 270
BsrFI r'CCGG_y
Cuts at: 0 116 213 1143
Size: 116 97 930
Fragments arranged by size:
930 116 97
BssHII G'CGCG_C
Cuts at: 0 219 221 1143
Size: 219 2 922
Fragments arranged by size:
922 219 2
BssSI C'ACGA_G
Cuts at: 0 459 1143
Size: 459 684
BstEII G'GTnAC_C
Cuts at: 0 205 1143
Size: 205 938
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 672 1143
Size: 165 507 471
Fragments arranged by size:
507 471 165
BstYI r'GATC_y
Cuts at: 0 626 1143
Size: 626 517
BstZ17I GTA'TAC
Cuts at: 0 1116 1143
Size: 1116 27
Bsu36I CC'TnA_GG
Cuts at: 0 818 1143
Size: 818 325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 840 875 1143
Size: 840 35 268
Fragments arranged by size:
840 268 35
DraI TTT'AAA
Cuts at: 0 759 1143
Size: 759 384
EaeI y'GGCC_r
Cuts at: 0 118 155 167 1143
Size: 118 37 12 976
Fragments arranged by size:
976 118 37 12
EagI C'GGCC_G
Cuts at: 0 118 1143
Size: 118 1025
EarI CTCTTCn'nnn_
Cuts at: 0 232 531 1143
Size: 232 299 612
Fragments arranged by size:
612 299 232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 390 810 1143
Size: 100 290 420 333
Fragments arranged by size:
420 333 290 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 1143
Size: 199 944
EcoO109I rG'GnC_Cy
Cuts at: 0 504 1143
Size: 504 639
EcoRI G'AATT_C
Cuts at: 0 688 1143
Size: 688 455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 723 1143
Size: 723 420
HaeII r_GCGC'y
Cuts at: 0 195 267 1143
Size: 195 72 876
Fragments arranged by size:
876 195 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 514 526 546 558 1143
Size: 514 12 20 12 585
Fragments arranged by size:
585 514 20 12 12
KasI G'GCGC_C
Cuts at: 0 191 1143
Size: 191 952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 623 662 1007 1127 1143
Size: 14 609 39 345 120 16
Fragments arranged by size:
609 345 120 39 16 14
MscI TGG'CCA
Cuts at: 0 169 1143
Size: 169 974
MslI CAynn'nnrTG
Cuts at: 0 163 283 1143
Size: 163 120 860
Fragments arranged by size:
860 163 120
MspA1I CmG'CkG
Cuts at: 0 78 594 1017 1083 1143
Size: 78 516 423 66 60
Fragments arranged by size:
516 423 78 66 60
NaeI GCC'GGC
Cuts at: 0 118 1143
Size: 118 1025
NarI GG'CG_CC
Cuts at: 0 192 1143
Size: 192 951
NgoMIV G'CCGG_C
Cuts at: 0 116 1143
Size: 116 1027
PstI C_TGCA'G
Cuts at: 0 200 1143
Size: 200 943
PvuII CAG'CTG
Cuts at: 0 78 1017 1083 1143
Size: 78 939 66 60
Fragments arranged by size:
939 78 66 60
SapI GCTCTTCn'nnn_
Cuts at: 0 232 1143
Size: 232 911
SexAI A'CCwGG_T
Cuts at: 0 1118 1143
Size: 1118 25
SfcI C'TryA_G
Cuts at: 0 196 1143
Size: 196 947
SfoI GGC'GCC
Cuts at: 0 193 1143
Size: 193 950
SmaI CCC'GGG
Cuts at: 0 176 847 1143
Size: 176 671 296
Fragments arranged by size:
671 296 176
SmlI C'TyrA_G
Cuts at: 0 355 364 1143
Size: 355 9 779
Fragments arranged by size:
779 355 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 569 1143
Size: 569 574
TatI w'GTAC_w
Cuts at: 0 701 1143
Size: 701 442
Tth111I GACn'n_nGTC
Cuts at: 0 964 1143
Size: 964 179
XbaI T'CTAG_A
Cuts at: 0 450 1143
Size: 450 693
XmaI C'CCGG_G
Cuts at: 0 174 845 1143
Size: 174 671 298
Fragments arranged by size:
671 298 174
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BcgI BglII Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaHI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII
BstXI BstYI BstZ17I Bsu36I CspCI DraI EaeI EagI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PstI PvuII SapI SexAI SfcI SfoI SmaI SmlI
TaqII TatI Tth111I XbaI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BciVI BclI BfrBI BglI BlpI BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BstAPI BstBI
BtgI BtgZI BtsI ClaI DraIII DrdI EciI EcoICRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TstI XcmI XhoI XmnI ZraI