Restriction map for
pF1KB6501
(Linear) (Six-base) MAPSORT of: KIBB6501.seq Check: 7595 from: 1 to: 1074
KIBB6501.orf
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AatII G_ACGT'C
Cuts at: 0 254 1074
Size: 254 820
AccI GT'mk_AC
Cuts at: 0 506 593 1074
Size: 506 87 481
Fragments arranged by size:
506 481 87
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 387 967 1074
Size: 322 65 580 107
Fragments arranged by size:
580 322 107 65
AfeI AGC'GCT
Cuts at: 0 358 1074
Size: 358 716
AflIII A'CryG_T
Cuts at: 0 276 1074
Size: 276 798
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 559 591 1074
Size: 559 32 483
Fragments arranged by size:
559 483 32
ApaI G_GGCC'C
Cuts at: 0 687 1074
Size: 687 387
ApaLI G'TGCA_C
Cuts at: 0 187 1074
Size: 187 887
ApoI r'AATT_y
Cuts at: 0 764 1068 1074
Size: 764 304 6
Fragments arranged by size:
764 304 6
AvaI C'yCGr_G
Cuts at: 0 10 622 1074
Size: 10 612 452
Fragments arranged by size:
612 452 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 610 643 1074
Size: 610 33 431
Fragments arranged by size:
610 431 33
BanI G'GyrC_C
Cuts at: 0 6 78 1074
Size: 6 72 996
Fragments arranged by size:
996 72 6
BanII G_rGCy'C
Cuts at: 0 401 449 687 1074
Size: 401 48 238 387
Fragments arranged by size:
401 387 238 48
BbeI G_GCGC'C
Cuts at: 0 10 82 1074
Size: 10 72 992
Fragments arranged by size:
992 72 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 1074
Size: 211 863
BglI GCCn_nnn'nGGC
Cuts at: 0 25 1074
Size: 25 1049
Bme1580I G_kGCm'C
Cuts at: 0 169 191 687 965 1074
Size: 169 22 496 278 109
Fragments arranged by size:
496 278 169 109 22
BmgBI CAC'GTC
Cuts at: 0 344 1074
Size: 344 730
BmrI ACTGGGnnnn_n'
Cuts at: 0 179 930 1029 1074
Size: 179 751 99 45
Fragments arranged by size:
751 179 99 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 1074
Size: 60 1014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 1074
Size: 417 657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1074
Size: 214 860
BsaI GGTCTCn'nnnn_
Cuts at: 0 58 1074
Size: 58 1016
BsaAI yAC'GTr
Cuts at: 0 279 384 1074
Size: 279 105 690
Fragments arranged by size:
690 279 105
BsaHI Gr'CG_yC
Cuts at: 0 7 79 251 1074
Size: 7 72 172 823
Fragments arranged by size:
823 172 72 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 1074
Size: 475 30 569
Fragments arranged by size:
569 475 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 1074
Size: 76 998
BseYI C'CCAG_C
Cuts at: 0 94 1074
Size: 94 980
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1074
Size: 472 602
BsiEI CG_ry'CG
Cuts at: 0 29 1074
Size: 29 1045
BsiHKAI G_wGCw'C
Cuts at: 0 191 401 1074
Size: 191 210 673
Fragments arranged by size:
673 210 191
BsmI GAATG_Cn'
Cuts at: 0 242 1074
Size: 242 832
Bsp1286I G_dGCh'C
Cuts at: 0 169 191 401 449 687 965 1074
Size: 169 22 210 48 238 278 109
Fragments arranged by size:
278 238 210 169 109 48 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 419 494 1074
Size: 419 75 580
Fragments arranged by size:
580 419 75
BsrBI CCG'CTC
Cuts at: 0 911 1074
Size: 911 163
BssSI C'ACGA_G
Cuts at: 0 835 1074
Size: 835 239
BstBI TT'CG_AA
Cuts at: 0 998 1074
Size: 998 76
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 798 1074
Size: 588 210 276
Fragments arranged by size:
588 276 210
Bsu36I CC'TnA_GG
Cuts at: 0 101 1074
Size: 101 973
BtgI C'CryG_G
Cuts at: 0 29 161 440 660 671 914 1074
Size: 29 132 279 220 11 243 160
Fragments arranged by size:
279 243 220 160 132 29 11
DraIII CAC_nnn'GTG
Cuts at: 0 195 1074
Size: 195 879
EaeI y'GGCC_r
Cuts at: 0 26 155 549 1074
Size: 26 129 394 525
Fragments arranged by size:
525 394 129 26
EagI C'GGCC_G
Cuts at: 0 26 1074
Size: 26 1048
EarI CTCTTCn'nnn_
Cuts at: 0 406 454 1074
Size: 406 48 620
Fragments arranged by size:
620 406 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 73 247 1074
Size: 49 24 174 827
Fragments arranged by size:
827 174 49 24
EcoICRI GAG'CTC
Cuts at: 0 399 1074
Size: 399 675
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 387 417 967 1074
Size: 322 65 30 550 107
Fragments arranged by size:
550 322 107 65 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 265 1074
Size: 196 69 809
Fragments arranged by size:
809 196 69
EcoO109I rG'GnC_Cy
Cuts at: 0 684 1074
Size: 684 390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 237 1074
Size: 237 837
FspI TGC'GCA
Cuts at: 0 415 1074
Size: 415 659
HaeII r_GCGC'y
Cuts at: 0 10 82 109 360 429 1074
Size: 10 72 27 251 69 645
Fragments arranged by size:
645 251 72 69 27 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 1074
Size: 94 32 948
Fragments arranged by size:
948 94 32
HincII GTy'rAC
Cuts at: 0 843 1074
Size: 843 231
KasI G'GCGC_C
Cuts at: 0 6 78 1074
Size: 6 72 996
Fragments arranged by size:
996 72 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1074
Size: 1016 58
MscI TGG'CCA
Cuts at: 0 157 551 1074
Size: 157 394 523
Fragments arranged by size:
523 394 157
MslI CAynn'nnrTG
Cuts at: 0 373 1074
Size: 373 701
MspA1I CmG'CkG
Cuts at: 0 31 1074
Size: 31 1043
NarI GG'CG_CC
Cuts at: 0 7 79 1074
Size: 7 72 995
Fragments arranged by size:
995 72 7
NotI GC'GGCC_GC
Cuts at: 0 26 1074
Size: 26 1048
NspI r_CATG'y
Cuts at: 0 372 1074
Size: 372 702
PspOMI G'GGCC_C
Cuts at: 0 683 1074
Size: 683 391
PstI C_TGCA'G
Cuts at: 0 140 462 1074
Size: 140 322 612
Fragments arranged by size:
612 322 140
SacI G_AGCT'C
Cuts at: 0 401 1074
Size: 401 673
SacII CC_GC'GG
Cuts at: 0 32 1074
Size: 32 1042
SapI GCTCTTCn'nnn_
Cuts at: 0 406 454 1074
Size: 406 48 620
Fragments arranged by size:
620 406 48
SbfI CC_TGCA'GG
Cuts at: 0 140 1074
Size: 140 934
SexAI A'CCwGG_T
Cuts at: 0 191 1074
Size: 191 883
SfcI C'TryA_G
Cuts at: 0 136 458 507 1074
Size: 136 322 49 567
Fragments arranged by size:
567 322 136 49
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 25 1074
Size: 25 1049
SfoI GGC'GCC
Cuts at: 0 8 80 1074
Size: 8 72 994
Fragments arranged by size:
994 72 8
SmaI CCC'GGG
Cuts at: 0 12 1074
Size: 12 1062
SmlI C'TyrA_G
Cuts at: 0 229 1074
Size: 229 845
SrfI GCCC'GGGC
Cuts at: 0 12 1074
Size: 12 1062
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 597 629 656 688 1074
Size: 597 32 27 32 386
Fragments arranged by size:
597 386 32 32 27
XmaI C'CCGG_G
Cuts at: 0 10 1074
Size: 10 1064
XmnI GAAnn'nnTTC
Cuts at: 0 644 929 1074
Size: 644 285 145
Fragments arranged by size:
644 285 145
ZraI GAC'GTC
Cuts at: 0 252 1074
Size: 252 822
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AloI ApaI ApaLI
ApoI AvaI BaeI BanI BanII BbeI BbsI BglI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BssSI BstBI BstXI Bsu36I BtgI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NotI NspI PspOMI PstI
SacI SacII SapI SbfI SexAI SfcI SfiI SfoI
SmaI SmlI SrfI TstI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AleI AlfI
AlwNI AscI AseI AsiSI AvrII BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI Bpu10I BsaBI
BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BstAPI BstEII BstYI BstZ17I BtgZI BtsI ClaI
CspCI DraI DrdI EcoRI EcoRV FseI FspAI HindIII
HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI SanDI ScaI SgfI SgrAI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI