(Linear) (Six-base) MAPSORT of: KIBB6498.seq  Check: 7826  from: 1  to: 183



KIBB6498.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      49     183 
   Size:         49     134
 
AlwNI CAG_nnn'CTG

Cuts at:      0     176     183 
   Size:        176       7
 
BamHI G'GATC_C

Cuts at:      0       3     183 
   Size:          3     180
 
BglI GCCn_nnn'nGGC

Cuts at:      0     116     183 
   Size:        116      67
 
Bme1580I G_kGCm'C

Cuts at:      0     135     183 
   Size:        135      48
 
BsaHI Gr'CG_yC

Cuts at:      0     157     183 
   Size:        157      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     183 
   Size:         21     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     135     183 
   Size:        135      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      49     183 
   Size:         49     134
 
BsrFI r'CCGG_y

Cuts at:      0      25      46     183 
   Size:         25      21     137

  Fragments arranged by size: 

                 137      25      21
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      68      83     176     183 
   Size:         68      15      93       7

  Fragments arranged by size: 

                  93      68      15       7
 
BstYI r'GATC_y

Cuts at:      0       3     183 
   Size:          3     180
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     183 
   Size:        140      43
 
MspA1I CmG'CkG

Cuts at:      0     173     183 
   Size:        173      10
 
NaeI GCC'GGC

Cuts at:      0      48     183 
   Size:         48     135
 
NgoMIV G'CCGG_C

Cuts at:      0      46     183 
   Size:         46     137
 
PasI CC'CwG_GG

Cuts at:      0     135     183 
   Size:        135      48
 
PvuII CAG'CTG

Cuts at:      0     173     183 
   Size:        173      10
 
SgrAI Cr'CCGG_yG

Cuts at:      0      25     183 
   Size:         25     158
 

 Enzymes that do cut:

     AarI    AlwNI    BamHI     BglI Bme1580I    BsaHI     BsgI Bsp1286I
    BspMI    BsrFI   BstAPI    BstYI     MmeI   MspA1I     NaeI   NgoMIV
     PasI    PvuII    SgrAI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI