Restriction map for
pF1KB6496
(Linear) (Six-base) MAPSORT of: KIBB6496.seq Check: 3503 from: 1 to: 1074
KIBB6496.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 1052 1074
Size: 1052 22
AleI CACnn'nnGTG
Cuts at: 0 781 1074
Size: 781 293
AlwNI CAG_nnn'CTG
Cuts at: 0 15 207 1026 1074
Size: 15 192 819 48
Fragments arranged by size:
819 192 48 15
ApaI G_GGCC'C
Cuts at: 0 544 1074
Size: 544 530
ApoI r'AATT_y
Cuts at: 0 196 444 1074
Size: 196 248 630
Fragments arranged by size:
630 248 196
AvaI C'yCGr_G
Cuts at: 0 863 1074
Size: 863 211
BanI G'GyrC_C
Cuts at: 0 93 1074
Size: 93 981
BanII G_rGCy'C
Cuts at: 0 301 544 917 949 1074
Size: 301 243 373 32 125
Fragments arranged by size:
373 301 243 125 32
BglII A'GATC_T
Cuts at: 0 83 408 1074
Size: 83 325 666
Fragments arranged by size:
666 325 83
Bme1580I G_kGCm'C
Cuts at: 0 544 1074
Size: 544 530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1074
Size: 728 346
Bpu10I CC'TnA_GC
Cuts at: 0 16 1074
Size: 16 1058
BsaAI yAC'GTr
Cuts at: 0 693 1074
Size: 693 381
BsaBI GATnn'nnATC
Cuts at: 0 290 1074
Size: 290 784
BsaWI w'CCGG_w
Cuts at: 0 164 1074
Size: 164 910
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 776 1074
Size: 776 298
BseYI C'CCAG_C
Cuts at: 0 533 537 1074
Size: 533 4 537
Fragments arranged by size:
537 533 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1074
Size: 1063 11
BsiEI CG_ry'CG
Cuts at: 0 165 1074
Size: 165 909
BsiHKAI G_wGCw'C
Cuts at: 0 22 301 1074
Size: 22 279 773
Fragments arranged by size:
773 279 22
BsmI GAATG_Cn'
Cuts at: 0 589 1074
Size: 589 485
BsmBI CGTCTCn'nnnn_
Cuts at: 0 644 1074
Size: 644 430
Bsp1286I G_dGCh'C
Cuts at: 0 22 301 544 917 949 1074
Size: 22 279 243 373 32 125
Fragments arranged by size:
373 279 243 125 32 22
BsrGI T'GTAC_A
Cuts at: 0 773 1074
Size: 773 301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 26 522 1074
Size: 26 496 552
Fragments arranged by size:
552 496 26
BstXI CCAn_nnnn'nTGG
Cuts at: 0 537 1074
Size: 537 537
BstYI r'GATC_y
Cuts at: 0 83 204 408 1074
Size: 83 121 204 666
Fragments arranged by size:
666 204 121 83
BstZ17I GTA'TAC
Cuts at: 0 1053 1074
Size: 1053 21
BtgI C'CryG_G
Cuts at: 0 303 1074
Size: 303 771
EcoICRI GAG'CTC
Cuts at: 0 299 1074
Size: 299 775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1074
Size: 728 346
EcoO109I rG'GnC_Cy
Cuts at: 0 541 1074
Size: 541 533
HaeII r_GCGC'y
Cuts at: 0 552 1074
Size: 552 522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 998 1030 1074
Size: 214 32 752 32 44
Fragments arranged by size:
752 214 44 32 32
HincII GTy'rAC
Cuts at: 0 992 1074
Size: 992 82
HpaI GTT'AAC
Cuts at: 0 992 1074
Size: 992 82
MfeI C'AATT_G
Cuts at: 0 507 1074
Size: 507 567
MslI CAynn'nnrTG
Cuts at: 0 695 781 839 1074
Size: 695 86 58 235
Fragments arranged by size:
695 235 86 58
MspA1I CmG'CkG
Cuts at: 0 537 1074
Size: 537 537
NcoI C'CATG_G
Cuts at: 0 303 1074
Size: 303 771
PasI CC'CwG_GG
Cuts at: 0 544 1074
Size: 544 530
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 570 1074
Size: 207 363 504
Fragments arranged by size:
504 363 207
PfoI T'CCnGG_A
Cuts at: 0 200 1074
Size: 200 874
PmlI CAC'GTG
Cuts at: 0 693 1074
Size: 693 381
PspOMI G'GGCC_C
Cuts at: 0 540 1074
Size: 540 534
PstI C_TGCA'G
Cuts at: 0 152 1074
Size: 152 922
PvuII CAG'CTG
Cuts at: 0 537 1074
Size: 537 537
SacI G_AGCT'C
Cuts at: 0 301 1074
Size: 301 773
SfcI C'TryA_G
Cuts at: 0 132 148 1074
Size: 132 16 926
Fragments arranged by size:
926 132 16
SmaI CCC'GGG
Cuts at: 0 865 1074
Size: 865 209
SpeI A'CTAG_T
Cuts at: 0 600 1074
Size: 600 474
SspI AAT'ATT
Cuts at: 0 381 749 1074
Size: 381 368 325
Fragments arranged by size:
381 368 325
StuI AGG'CCT
Cuts at: 0 130 1074
Size: 130 944
StyI C'CwwG_G
Cuts at: 0 303 1074
Size: 303 771
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 547 758 1074
Size: 199 348 211 316
Fragments arranged by size:
348 316 211 199
TatI w'GTAC_w
Cuts at: 0 773 1074
Size: 773 301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 434 466 1074
Size: 434 32 608
Fragments arranged by size:
608 434 32
XbaI T'CTAG_A
Cuts at: 0 411 1074
Size: 411 663
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 541 1074
Size: 541 533
XmaI C'CCGG_G
Cuts at: 0 863 1074
Size: 863 211
Enzymes that do cut:
AccI AleI AlwNI ApaI ApoI AvaI BanI BanII
BglII Bme1580I BpmI Bpu10I BsaAI BsaBI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrGI
BstAPI BstXI BstYI BstZ17I BtgI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII HpaI MfeI MslI MspA1I NcoI
PasI PflMI PfoI PmlI PspOMI PstI PvuII SacI
SfcI SmaI SpeI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaHI BsaXI BsiWI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII KasI KpnI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SphI SrfI SwaI Tth111I XhoI XmnI ZraI