Restriction map for
pF1KB6486
(Linear) (Six-base) MAPSORT of: KIBB6486.seq Check: 8160 from: 1 to: 1068
KIBB6486.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 591 1068
Size: 591 477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1068
Size: 700 368
AflII C'TTAA_G
Cuts at: 0 712 1068
Size: 712 356
AlwNI CAG_nnn'CTG
Cuts at: 0 1014 1017 1068
Size: 1014 3 51
Fragments arranged by size:
1014 51 3
ApoI r'AATT_y
Cuts at: 0 12 879 1068
Size: 12 867 189
Fragments arranged by size:
867 189 12
BanI G'GyrC_C
Cuts at: 0 80 1068
Size: 80 988
BanII G_rGCy'C
Cuts at: 0 977 1068
Size: 977 91
BbvCI CC'TCA_GC
Cuts at: 0 437 1031 1068
Size: 437 594 37
Fragments arranged by size:
594 437 37
BclI T'GATC_A
Cuts at: 0 830 1068
Size: 830 238
Bme1580I G_kGCm'C
Cuts at: 0 491 1068
Size: 491 577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 561 1068
Size: 54 507 507
Fragments arranged by size:
507 507 54
Bpu10I CC'TnA_GC
Cuts at: 0 428 437 971 1031 1068
Size: 428 9 534 60 37
Fragments arranged by size:
534 428 60 37 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 1068
Size: 130 938
BseYI C'CCAG_C
Cuts at: 0 442 757 1068
Size: 442 315 311
Fragments arranged by size:
442 315 311
BsiHKAI G_wGCw'C
Cuts at: 0 977 1068
Size: 977 91
BsmBI CGTCTCn'nnnn_
Cuts at: 0 27 1068
Size: 27 1041
Bsp1286I G_dGCh'C
Cuts at: 0 491 977 1068
Size: 491 486 91
Fragments arranged by size:
491 486 91
BspHI T'CATG_A
Cuts at: 0 999 1068
Size: 999 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 573 591 1068
Size: 210 363 18 477
Fragments arranged by size:
477 363 210 18
BsrFI r'CCGG_y
Cuts at: 0 77 1068
Size: 77 991
BsrGI T'GTAC_A
Cuts at: 0 816 1068
Size: 816 252
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1017 1068
Size: 1017 51
BtgI C'CryG_G
Cuts at: 0 658 1068
Size: 658 410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 225 260 1068
Size: 225 35 808
Fragments arranged by size:
808 225 35
DraIII CAC_nnn'GTG
Cuts at: 0 309 729 1068
Size: 309 420 339
Fragments arranged by size:
420 339 309
EaeI y'GGCC_r
Cuts at: 0 291 474 689 1068
Size: 291 183 215 379
Fragments arranged by size:
379 291 215 183
EarI CTCTTCn'nnn_
Cuts at: 0 341 376 1068
Size: 341 35 692
Fragments arranged by size:
692 341 35
EcoICRI GAG'CTC
Cuts at: 0 975 1068
Size: 975 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 561 700 1068
Size: 54 507 139 368
Fragments arranged by size:
507 368 139 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 1068
Size: 496 572
EcoO109I rG'GnC_Cy
Cuts at: 0 570 1068
Size: 570 498
EcoRI G'AATT_C
Cuts at: 0 879 1068
Size: 879 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 938 970 1068
Size: 938 32 98
Fragments arranged by size:
938 98 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 1068
Size: 50 1018
MscI TGG'CCA
Cuts at: 0 293 476 691 1068
Size: 293 183 215 377
Fragments arranged by size:
377 293 215 183
MslI CAynn'nnrTG
Cuts at: 0 824 1068
Size: 824 244
MspA1I CmG'CkG
Cuts at: 0 1014 1035 1068
Size: 1014 21 33
Fragments arranged by size:
1014 33 21
NaeI GCC'GGC
Cuts at: 0 79 1068
Size: 79 989
NdeI CA'TA_TG
Cuts at: 0 719 1068
Size: 719 349
NgoMIV G'CCGG_C
Cuts at: 0 77 1068
Size: 77 991
PasI CC'CwG_GG
Cuts at: 0 531 1068
Size: 531 537
PflMI CCAn_nnn'nTGG
Cuts at: 0 648 1068
Size: 648 420
PpuMI rG'GwC_Cy
Cuts at: 0 570 1068
Size: 570 498
PstI C_TGCA'G
Cuts at: 0 287 1068
Size: 287 781
PvuII CAG'CTG
Cuts at: 0 1014 1035 1068
Size: 1014 21 33
Fragments arranged by size:
1014 33 21
SacI G_AGCT'C
Cuts at: 0 977 1068
Size: 977 91
SfcI C'TryA_G
Cuts at: 0 283 410 1068
Size: 283 127 658
Fragments arranged by size:
658 283 127
SmlI C'TyrA_G
Cuts at: 0 712 1068
Size: 712 356
TatI w'GTAC_w
Cuts at: 0 174 816 1068
Size: 174 642 252
Fragments arranged by size:
642 252 174
Tth111I GACn'n_nGTC
Cuts at: 0 250 1068
Size: 250 818
XmnI GAAnn'nnTTC
Cuts at: 0 883 1068
Size: 883 185
Enzymes that do cut:
AarI AcuI AflII AlwNI ApoI BanI BanII BbvCI
BclI Bme1580I BpmI Bpu10I BsaI BseYI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI BtgI CspCI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
Hin4I MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
PasI PflMI PpuMI PstI PvuII SacI SfcI SmlI
TatI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiWI BsmI BspEI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI DraI DrdI EagI EciI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI XbaI XcmI XhoI XmaI
ZraI