Restriction map for
pF1KB6479
(Linear) (Six-base) MAPSORT of: KIBB6479.seq Check: 9342 from: 1 to: 981
KIBB6479.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 529 981
Size: 529 452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 185 981
Size: 100 85 796
Fragments arranged by size:
796 100 85
AhdI GACnn_n'nnGTC
Cuts at: 0 525 981
Size: 525 456
ApaLI G'TGCA_C
Cuts at: 0 479 981
Size: 479 502
ApoI r'AATT_y
Cuts at: 0 46 772 934 948 981
Size: 46 726 162 14 33
Fragments arranged by size:
726 162 46 33 14
BanI G'GyrC_C
Cuts at: 0 510 981
Size: 510 471
BanII G_rGCy'C
Cuts at: 0 505 981
Size: 505 476
BbeI G_GCGC'C
Cuts at: 0 514 981
Size: 514 467
BbsI GAAGACnn'nnnn_
Cuts at: 0 106 166 981
Size: 106 60 815
Fragments arranged by size:
815 106 60
BciVI GTATCCnnnnn_n'
Cuts at: 0 83 981
Size: 83 898
BglII A'GATC_T
Cuts at: 0 833 981
Size: 833 148
Bme1580I G_kGCm'C
Cuts at: 0 483 981
Size: 483 498
BmrI ACTGGGnnnn_n'
Cuts at: 0 603 981
Size: 603 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 938 981
Size: 907 31 43
Fragments arranged by size:
907 43 31
BsaAI yAC'GTr
Cuts at: 0 637 981
Size: 637 344
BsaHI Gr'CG_yC
Cuts at: 0 511 981
Size: 511 470
BsaWI w'CCGG_w
Cuts at: 0 321 981
Size: 321 660
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 238 981
Size: 138 100 743
Fragments arranged by size:
743 138 100
BsiHKAI G_wGCw'C
Cuts at: 0 483 505 981
Size: 483 22 476
Fragments arranged by size:
483 476 22
Bsp1286I G_dGCh'C
Cuts at: 0 483 505 981
Size: 483 22 476
Fragments arranged by size:
483 476 22
BsrDI GCAATG_nn'
Cuts at: 0 557 981
Size: 557 424
BstYI r'GATC_y
Cuts at: 0 833 981
Size: 833 148
Bsu36I CC'TnA_GG
Cuts at: 0 515 981
Size: 515 466
BtgI C'CryG_G
Cuts at: 0 729 981
Size: 729 252
BtsI GCAGTG_nn'
Cuts at: 0 481 981
Size: 481 500
DraI TTT'AAA
Cuts at: 0 978 981
Size: 978 3
EarI CTCTTCn'nnn_
Cuts at: 0 843 981
Size: 843 138
EcoICRI GAG'CTC
Cuts at: 0 503 981
Size: 503 478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 185 907 938 981
Size: 100 85 722 31 43
Fragments arranged by size:
722 100 85 43 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 153 981
Size: 153 828
EcoO109I rG'GnC_Cy
Cuts at: 0 736 981
Size: 736 245
EcoRV GAT'ATC
Cuts at: 0 557 981
Size: 557 424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 593 981
Size: 593 388
HaeII r_GCGC'y
Cuts at: 0 514 981
Size: 514 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 684 716 782 814 820 852
Size: 511 32 141 32 66 32 6 32
Cuts at: 852 981
Size: 129
Fragments arranged by size:
511 141 129 66 32 32 32 32
6
KasI G'GCGC_C
Cuts at: 0 510 981
Size: 510 471
MspA1I CmG'CkG
Cuts at: 0 731 981
Size: 731 250
NarI GG'CG_CC
Cuts at: 0 511 981
Size: 511 470
PfoI T'CCnGG_A
Cuts at: 0 559 800 884 981
Size: 559 241 84 97
Fragments arranged by size:
559 241 97 84
PpuMI rG'GwC_Cy
Cuts at: 0 736 981
Size: 736 245
SacI G_AGCT'C
Cuts at: 0 505 981
Size: 505 476
SacII CC_GC'GG
Cuts at: 0 732 981
Size: 732 249
SanDI GG'GwC_CC
Cuts at: 0 736 981
Size: 736 245
SfcI C'TryA_G
Cuts at: 0 842 981
Size: 842 139
SfoI GGC'GCC
Cuts at: 0 512 981
Size: 512 469
StyI C'CwwG_G
Cuts at: 0 362 568 652 854 967 981
Size: 362 206 84 202 113 14
Fragments arranged by size:
362 206 202 113 84 14
Enzymes that do cut:
AccI AcuI AhdI ApaLI ApoI BanI BanII BbeI
BbsI BciVI BglII Bme1580I BmrI BpmI BsaAI BsaHI
BsaWI BseRI BsiHKAI Bsp1286I BsrDI BstYI Bsu36I BtgI
BtsI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I KasI MspA1I NarI PfoI PpuMI
SacI SacII SanDI SfcI SfoI StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI
BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoRI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI