Restriction map for
pF1KB6462
(Linear) (Six-base) MAPSORT of: KIBB6462.seq Check: 9914 from: 1 to: 1056
KIBB6462.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 489 1056
Size: 489 567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 412 839 1056
Size: 92 320 427 217
Fragments arranged by size:
427 320 217 92
AlwNI CAG_nnn'CTG
Cuts at: 0 696 1056
Size: 696 360
ApoI r'AATT_y
Cuts at: 0 295 458 565 1034 1056
Size: 295 163 107 469 22
Fragments arranged by size:
469 295 163 107 22
BanII G_rGCy'C
Cuts at: 0 891 1056
Size: 891 165
BclI T'GATC_A
Cuts at: 0 209 1056
Size: 209 847
BglI GCCn_nnn'nGGC
Cuts at: 0 1007 1056
Size: 1007 49
BglII A'GATC_T
Cuts at: 0 447 1056
Size: 447 609
BlpI GC'TnA_GC
Cuts at: 0 197 202 662 1056
Size: 197 5 460 394
Fragments arranged by size:
460 394 197 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 217 1056
Size: 217 839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 808 821 1056
Size: 688 120 13 235
Fragments arranged by size:
688 235 120 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 1056
Size: 1 1055
BsaI GGTCTCn'nnnn_
Cuts at: 0 832 1056
Size: 832 224
BsaHI Gr'CG_yC
Cuts at: 0 492 1056
Size: 492 564
BsaWI w'CCGG_w
Cuts at: 0 534 1056
Size: 534 522
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 828 858 1056
Size: 828 30 198
Fragments arranged by size:
828 198 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 1056
Size: 80 976
BseYI C'CCAG_C
Cuts at: 0 333 1056
Size: 333 723
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1056
Size: 632 424
BsiHKAI G_wGCw'C
Cuts at: 0 891 1056
Size: 891 165
Bsp1286I G_dGCh'C
Cuts at: 0 891 1056
Size: 891 165
BspEI T'CCGG_A
Cuts at: 0 534 1056
Size: 534 522
BspHI T'CATG_A
Cuts at: 0 438 1056
Size: 438 618
BsrFI r'CCGG_y
Cuts at: 0 10 1056
Size: 10 1046
BstYI r'GATC_y
Cuts at: 0 447 692 1056
Size: 447 245 364
Fragments arranged by size:
447 364 245
Bsu36I CC'TnA_GG
Cuts at: 0 260 1056
Size: 260 796
BtgI C'CryG_G
Cuts at: 0 231 1056
Size: 231 825
DraI TTT'AAA
Cuts at: 0 933 1056
Size: 933 123
DraIII CAC_nnn'GTG
Cuts at: 0 343 1056
Size: 343 713
EaeI y'GGCC_r
Cuts at: 0 8 234 999 1056
Size: 8 226 765 57
Fragments arranged by size:
765 226 57 8
EarI CTCTTCn'nnn_
Cuts at: 0 297 431 536 1056
Size: 297 134 105 520
Fragments arranged by size:
520 297 134 105
EcoICRI GAG'CTC
Cuts at: 0 889 1056
Size: 889 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 412 688 808 821 839 1056
Size: 92 320 276 120 13 18 217
Fragments arranged by size:
320 276 217 120 92 18 13
EcoRV GAT'ATC
Cuts at: 0 945 1056
Size: 945 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 755 1056
Size: 429 326 301
Fragments arranged by size:
429 326 301
FseI GG_CCGG'CC
Cuts at: 0 14 1056
Size: 14 1042
HaeII r_GCGC'y
Cuts at: 0 274 1056
Size: 274 782
HincII GTy'rAC
Cuts at: 0 490 1056
Size: 490 566
MfeI C'AATT_G
Cuts at: 0 161 1056
Size: 161 895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 353 461 820 1056
Size: 42 311 108 359 236
Fragments arranged by size:
359 311 236 108 42
MscI TGG'CCA
Cuts at: 0 236 1001 1056
Size: 236 765 55
Fragments arranged by size:
765 236 55
MslI CAynn'nnrTG
Cuts at: 0 400 1056
Size: 400 656
MspA1I CmG'CkG
Cuts at: 0 666 1056
Size: 666 390
NaeI GCC'GGC
Cuts at: 0 12 1056
Size: 12 1044
NgoMIV G'CCGG_C
Cuts at: 0 10 1056
Size: 10 1046
NspI r_CATG'y
Cuts at: 0 603 1056
Size: 603 453
PflMI CCAn_nnn'nTGG
Cuts at: 0 340 1056
Size: 340 716
PsiI TTA'TAA
Cuts at: 0 509 1056
Size: 509 547
PstI C_TGCA'G
Cuts at: 0 701 1056
Size: 701 355
PvuII CAG'CTG
Cuts at: 0 666 1056
Size: 666 390
SacI G_AGCT'C
Cuts at: 0 891 1056
Size: 891 165
SalI G'TCGA_C
Cuts at: 0 488 1056
Size: 488 568
SapI GCTCTTCn'nnn_
Cuts at: 0 536 1056
Size: 536 520
ScaI AGT'ACT
Cuts at: 0 810 1056
Size: 810 246
SfcI C'TryA_G
Cuts at: 0 697 1056
Size: 697 359
SmlI C'TyrA_G
Cuts at: 0 16 1056
Size: 16 1040
TatI w'GTAC_w
Cuts at: 0 808 1056
Size: 808 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 828 860 1056
Size: 828 32 196
Fragments arranged by size:
828 196 32
XbaI T'CTAG_A
Cuts at: 0 558 1056
Size: 558 498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1026 1056
Size: 1026 30
XmnI GAAnn'nnTTC
Cuts at: 0 25 170 545 1056
Size: 25 145 375 511
Fragments arranged by size:
511 375 145 25
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanII BclI BglI BglII
BlpI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BsrFI
BstYI Bsu36I BtgI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoRV FalI FseI HaeII HincII MfeI MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PflMI PsiI
PstI PvuII SacI SalI SapI ScaI SfcI SmlI
TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BfrBI Bme1580I BmgBI BmrI BmtI
Bpu10I BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DrdI EagI
EciI EcoNI EcoO109I EcoRI FspI FspAI Hin4I HindIII
HpaI KasI KpnI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII Tth111I XhoI XmaI ZraI