(Linear) (Six-base) MAPSORT of: KIBB6454.seq  Check: 5085  from: 1  to: 1050



KIBB6454.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     567    1050 
   Size:        567     483
 
AclI AA'CG_TT

Cuts at:      0     434    1050 
   Size:        434     616
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1024    1050 
   Size:       1024      26
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     850    1050 
   Size:        850     200
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1024    1050 
   Size:       1024      26
 
ApoI r'AATT_y

Cuts at:      0     427     626    1050 
   Size:        427     199     424

  Fragments arranged by size: 

                 427     424     199
 
AseI AT'TA_AT

Cuts at:      0     545    1050 
   Size:        545     505
 
BanII G_rGCy'C

Cuts at:      0     267    1050 
   Size:        267     783
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     625    1050 
   Size:        625     425
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      29      63    1050 
   Size:         29      34     987

  Fragments arranged by size: 

                 987      34      29
 
BlpI GC'TnA_GC

Cuts at:      0     756    1050 
   Size:        756     294
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     351     658    1050 
   Size:        289      62     307     392

  Fragments arranged by size: 

                 392     307     289      62
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773    1050 
   Size:        773     277
 
BsaHI Gr'CG_yC

Cuts at:      0     326    1050 
   Size:        326     724
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     169     284    1050 
   Size:        169     115     766

  Fragments arranged by size: 

                 766     169     115
 
Bsp1286I G_dGCh'C

Cuts at:      0     267    1050 
   Size:        267     783
 
BspHI T'CATG_A

Cuts at:      0     882    1050 
   Size:        882     168
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     567    1050 
   Size:        567     483
 
BtsI GCAGTG_nn'

Cuts at:      0     479    1050 
   Size:        479     571
 
EarI CTCTTCn'nnn_

Cuts at:      0     124    1043    1050 
   Size:        124     919       7

  Fragments arranged by size: 

                 919     124       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     188    1050 
   Size:        188     862
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     351     658    1024    1050 
   Size:        289      62     307     366      26

  Fragments arranged by size: 

                 366     307     289      62      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     139    1050 
   Size:        139     911
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1032    1050 
   Size:       1032      18
 
EcoRI G'AATT_C

Cuts at:      0     427    1050 
   Size:        427     623
 
HaeII r_GCGC'y

Cuts at:      0     252    1050 
   Size:        252     798
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57     249     269     281     301     864     896 
   Size:         25      32     192      20      12      20     563      32

Cuts at:    896    1050 
   Size:        154

  Fragments arranged by size: 

                 563     192     154      32      32      25      20      20
                  12
 
HincII GTy'rAC

Cuts at:      0     330     438    1050 
   Size:        330     108     612

  Fragments arranged by size: 

                 612     330     108
 
HpaI GTT'AAC

Cuts at:      0     438    1050 
   Size:        438     612
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     234    1050 
   Size:         54     180     816

  Fragments arranged by size: 

                 816     180      54
 
PasI CC'CwG_GG

Cuts at:      0     321    1050 
   Size:        321     729
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     453    1050 
   Size:        453     597
 
PfoI T'CCnGG_A

Cuts at:      0     595    1050 
   Size:        595     455
 
PpuMI rG'GwC_Cy

Cuts at:      0    1032    1050 
   Size:       1032      18
 
PstI C_TGCA'G

Cuts at:      0     474     839    1050 
   Size:        474     365     211

  Fragments arranged by size: 

                 474     365     211
 
SanDI GG'GwC_CC

Cuts at:      0    1032    1050 
   Size:       1032      18
 
SfcI C'TryA_G

Cuts at:      0     404     470     835    1050 
   Size:        404      66     365     215

  Fragments arranged by size: 

                 404     365     215      66
 
SmlI C'TyrA_G

Cuts at:      0     788    1050 
   Size:        788     262
 
SspI AAT'ATT

Cuts at:      0    1008    1050 
   Size:       1008      42
 
StyI C'CwwG_G

Cuts at:      0     762     906    1050 
   Size:        762     144     144

  Fragments arranged by size: 

                 762     144     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     460    1050 
   Size:        460     590
 
TatI w'GTAC_w

Cuts at:      0     556    1050 
   Size:        556     494
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      68     100    1050 
   Size:         68      32     950

  Fragments arranged by size: 

                 950      68      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     460    1050 
   Size:        460     590
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AlfI    AlwNI     ApoI     AseI    BanII
     BbsI     BcgI     BlpI     BpmI    BpuEI    BsaHI    BseRI Bsp1286I
    BspHI    BspMI     BtsI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI    HaeII    Hin4I   HincII     HpaI     MmeI     PasI    PflMI
     PfoI    PpuMI     PstI    SanDI     SfcI     SmlI     SspI     StyI
    TaqII     TatI     TstI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI