(Linear) (Six-base) MAPSORT of: KIBB6440.seq  Check: 6603  from: 1  to: 1035



KIBB6440.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AleI CACnn'nnGTG

Cuts at:      0     817    1035 
   Size:        817     218
 
ApaLI G'TGCA_C

Cuts at:      0     369    1035 
   Size:        369     666
 
ApoI r'AATT_y

Cuts at:      0     853    1035 
   Size:        853     182
 
AvaI C'yCGr_G

Cuts at:      0      27     170    1035 
   Size:         27     143     865

  Fragments arranged by size: 

                 865     143      27
 
BanII G_rGCy'C

Cuts at:      0     394    1035 
   Size:        394     641
 
BfrBI ATG'CAT

Cuts at:      0    1015    1035 
   Size:       1015      20
 
Bme1580I G_kGCm'C

Cuts at:      0     373    1035 
   Size:        373     662
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     344     760    1035 
   Size:        344     416     275

  Fragments arranged by size: 

                 416     344     275
 
BsaAI yAC'GTr

Cuts at:      0     143    1035 
   Size:        143     892
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     821     851    1035 
   Size:        821      30     184

  Fragments arranged by size: 

                 821     184      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     188    1035 
   Size:        188     847
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     546    1035 
   Size:        546     489
 
BsiHKAI G_wGCw'C

Cuts at:      0      17     373     414    1035 
   Size:         17     356      41     621

  Fragments arranged by size: 

                 621     356      41      17
 
BsiWI C'GTAC_G

Cuts at:      0     282    1035 
   Size:        282     753
 
BsmI GAATG_Cn'

Cuts at:      0     601     872    1035 
   Size:        601     271     163

  Fragments arranged by size: 

                 601     271     163
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     373     394     414    1035 
   Size:         17     356      21      20     621

  Fragments arranged by size: 

                 621     356      21      20      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     568    1035 
   Size:        568     467
 
BsrGI T'GTAC_A

Cuts at:      0     235    1035 
   Size:        235     800
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     550    1035 
   Size:        550     485
 
DraIII CAC_nnn'GTG

Cuts at:      0     460    1035 
   Size:        460     575
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      65    1035 
   Size:         65     970
 
EarI CTCTTCn'nnn_

Cuts at:      0     128    1035 
   Size:        128     907
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     203    1035 
   Size:        203     832
 
HaeII r_GCGC'y

Cuts at:      0     356    1035 
   Size:        356     679
 
MslI CAynn'nnrTG

Cuts at:      0     817    1035 
   Size:        817     218
 
MspA1I CmG'CkG

Cuts at:      0     478    1035 
   Size:        478     557
 
NsiI A_TGCA'T

Cuts at:      0    1017    1035 
   Size:       1017      18
 
NspI r_CATG'y

Cuts at:      0     535    1035 
   Size:        535     500
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     478     637    1035 
   Size:        478     159     398

  Fragments arranged by size: 

                 478     398     159
 
PsiI TTA'TAA

Cuts at:      0     467    1035 
   Size:        467     568
 
PvuII CAG'CTG

Cuts at:      0     478    1035 
   Size:        478     557
 
SmlI C'TyrA_G

Cuts at:      0      27    1035 
   Size:         27    1008
 
SnaBI TAC'GTA

Cuts at:      0     143    1035 
   Size:        143     892
 
StyI C'CwwG_G

Cuts at:      0     825    1035 
   Size:        825     210
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     255    1035 
   Size:        255     780
 
TatI w'GTAC_w

Cuts at:      0     235    1035 
   Size:        235     800
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     901     925     933     957    1035 
   Size:        901      24       8      24      78

  Fragments arranged by size: 

                 901      78      24      24       8
 
XhoI C'TCGA_G

Cuts at:      0      27    1035 
   Size:         27    1008
 
XmnI GAAnn'nnTTC

Cuts at:      0     486    1035 
   Size:        486     549
 

 Enzymes that do cut:

     AleI    ApaLI     ApoI     AvaI    BanII    BfrBI Bme1580I     BmrI
    BsaAI    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspMI    BsrGI    BtgZI   DraIII     DrdI     EarI    EcoNI    HaeII
     MslI   MspA1I     NsiI     NspI    PflMI     PsiI    PvuII     SmlI
    SnaBI     StyI    TaqII     TatI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI     EaeI
     EagI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XmaI     ZraI