Restriction map for
pF1KB6433
(Linear) (Six-base) MAPSORT of: KIBB6433.seq Check: 3457 from: 1 to: 939
KIBB6433.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 265 939
Size: 265 674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 939
Size: 207 732
AleI CACnn'nnGTG
Cuts at: 0 439 610 939
Size: 439 171 329
Fragments arranged by size:
439 329 171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 273 939
Size: 273 666
ApaI G_GGCC'C
Cuts at: 0 416 775 791 939
Size: 416 359 16 148
Fragments arranged by size:
416 359 148 16
ApaLI G'TGCA_C
Cuts at: 0 911 939
Size: 911 28
ApoI r'AATT_y
Cuts at: 0 313 939
Size: 313 626
AvaI C'yCGr_G
Cuts at: 0 594 718 736 939
Size: 594 124 18 203
Fragments arranged by size:
594 203 124 18
BanI G'GyrC_C
Cuts at: 0 268 712 732 939
Size: 268 444 20 207
Fragments arranged by size:
444 268 207 20
BanII G_rGCy'C
Cuts at: 0 74 152 363 416 775 791 939
Size: 74 78 211 53 359 16 148
Fragments arranged by size:
359 211 148 78 74 53 16
BbeI G_GCGC'C
Cuts at: 0 716 939
Size: 716 223
BbsI GAAGACnn'nnnn_
Cuts at: 0 96 273 939
Size: 96 177 666
Fragments arranged by size:
666 177 96
BbvCI CC'TCA_GC
Cuts at: 0 193 939
Size: 193 746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 186 220 939
Size: 186 34 719
Fragments arranged by size:
719 186 34
BglI GCCn_nnn'nGGC
Cuts at: 0 266 939
Size: 266 673
BlpI GC'TnA_GC
Cuts at: 0 157 939
Size: 157 782
Bme1580I G_kGCm'C
Cuts at: 0 271 416 775 791 915 939
Size: 271 145 359 16 124 24
Fragments arranged by size:
359 271 145 124 24 16
BmgBI CAC'GTC
Cuts at: 0 24 939
Size: 24 915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 939
Size: 136 803
Bpu10I CC'TnA_GC
Cuts at: 0 193 939
Size: 193 746
BsaI GGTCTCn'nnnn_
Cuts at: 0 575 939
Size: 575 364
BsaHI Gr'CG_yC
Cuts at: 0 713 743 939
Size: 713 30 196
Fragments arranged by size:
713 196 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 939
Size: 291 648
BseYI C'CCAG_C
Cuts at: 0 43 680 939
Size: 43 637 259
Fragments arranged by size:
637 259 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 939
Size: 214 725
BsiHKAI G_wGCw'C
Cuts at: 0 152 641 915 939
Size: 152 489 274 24
Fragments arranged by size:
489 274 152 24
Bsp1286I G_dGCh'C
Cuts at: 0 74 152 271 363 416 641 775 791
Size: 74 78 119 92 53 225 134 16
Cuts at: 791 915 939
Size: 124 24
Fragments arranged by size:
225 134 124 119 92 78 74 53
24 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 265 939
Size: 265 674
BsrDI GCAATG_nn'
Cuts at: 0 103 939
Size: 103 836
BsrFI r'CCGG_y
Cuts at: 0 729 844 939
Size: 729 115 95
Fragments arranged by size:
729 115 95
BssHII G'CGCG_C
Cuts at: 0 699 701 939
Size: 699 2 238
Fragments arranged by size:
699 238 2
Bsu36I CC'TnA_GG
Cuts at: 0 809 872 939
Size: 809 63 67
Fragments arranged by size:
809 67 63
BtgI C'CryG_G
Cuts at: 0 326 353 434 939
Size: 326 27 81 505
Fragments arranged by size:
505 326 81 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 767 939
Size: 767 172
BtsI GCAGTG_nn'
Cuts at: 0 864 939
Size: 864 75
DraIII CAC_nnn'GTG
Cuts at: 0 237 939
Size: 237 702
EaeI y'GGCC_r
Cuts at: 0 329 365 431 506 939
Size: 329 36 66 75 433
Fragments arranged by size:
433 329 75 66 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 939
Size: 28 911
EcoICRI GAG'CTC
Cuts at: 0 150 939
Size: 150 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 207 939
Size: 136 71 732
Fragments arranged by size:
732 136 71
EcoO109I rG'GnC_Cy
Cuts at: 0 412 771 772 788 939
Size: 412 359 1 16 151
Fragments arranged by size:
412 359 151 16 1
EcoRI G'AATT_C
Cuts at: 0 313 939
Size: 313 626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 300 939
Size: 300 639
HaeII r_GCGC'y
Cuts at: 0 716 939
Size: 716 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 939
Size: 286 32 621
Fragments arranged by size:
621 286 32
HincII GTy'rAC
Cuts at: 0 838 939
Size: 838 101
KasI G'GCGC_C
Cuts at: 0 712 939
Size: 712 227
MscI TGG'CCA
Cuts at: 0 367 433 508 939
Size: 367 66 75 431
Fragments arranged by size:
431 367 75 66
MslI CAynn'nnrTG
Cuts at: 0 22 439 474 610 939
Size: 22 417 35 136 329
Fragments arranged by size:
417 329 136 35 22
MspA1I CmG'CkG
Cuts at: 0 36 156 851 939
Size: 36 120 695 88
Fragments arranged by size:
695 120 88 36
NaeI GCC'GGC
Cuts at: 0 846 939
Size: 846 93
NarI GG'CG_CC
Cuts at: 0 713 939
Size: 713 226
NcoI C'CATG_G
Cuts at: 0 353 939
Size: 353 586
NgoMIV G'CCGG_C
Cuts at: 0 844 939
Size: 844 95
NspI r_CATG'y
Cuts at: 0 131 569 939
Size: 131 438 370
Fragments arranged by size:
438 370 131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 939
Size: 193 32 714
Fragments arranged by size:
714 193 32
PshAI GACnn'nnGTC
Cuts at: 0 833 939
Size: 833 106
PspOMI G'GGCC_C
Cuts at: 0 412 771 787 939
Size: 412 359 16 152
Fragments arranged by size:
412 359 152 16
PstI C_TGCA'G
Cuts at: 0 386 939
Size: 386 553
PvuII CAG'CTG
Cuts at: 0 36 156 939
Size: 36 120 783
Fragments arranged by size:
783 120 36
SacI G_AGCT'C
Cuts at: 0 152 939
Size: 152 787
SexAI A'CCwGG_T
Cuts at: 0 632 939
Size: 632 307
SfcI C'TryA_G
Cuts at: 0 138 382 939
Size: 138 244 557
Fragments arranged by size:
557 244 138
SfoI GGC'GCC
Cuts at: 0 714 939
Size: 714 225
SmaI CCC'GGG
Cuts at: 0 738 939
Size: 738 201
StyI C'CwwG_G
Cuts at: 0 275 353 482 939
Size: 275 78 129 457
Fragments arranged by size:
457 275 129 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 196 267 930 939
Size: 196 71 663 9
Fragments arranged by size:
663 196 71 9
TatI w'GTAC_w
Cuts at: 0 239 939
Size: 239 700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 829 939
Size: 360 469 110
Fragments arranged by size:
469 360 110
XmaI C'CCGG_G
Cuts at: 0 736 939
Size: 736 203
Enzymes that do cut:
AarI AcuI AleI AlfI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII Bsu36I
BtgI BtgZI BtsI DraIII EaeI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PpiI
PshAI PspOMI PstI PvuII SacI SexAI SfcI SfoI
SmaI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I ClaI CspCI DraI DrdI
EagI EarI EcoNI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpuMI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI