(Linear) (Six-base) MAPSORT of: KIBB6432.seq  Check: 7123  from: 1  to: 1032



KIBB6432.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     698    1032 
   Size:        250     448     334

  Fragments arranged by size: 

                 448     334     250
 
AhdI GACnn_n'nnGTC

Cuts at:      0     791    1032 
   Size:        791     241
 
ApaLI G'TGCA_C

Cuts at:      0     218    1032 
   Size:        218     814
 
ApoI r'AATT_y

Cuts at:      0     352     541     683    1032 
   Size:        352     189     142     349

  Fragments arranged by size: 

                 352     349     189     142
 
AvaI C'yCGr_G

Cuts at:      0      27      77    1032 
   Size:         27      50     955

  Fragments arranged by size: 

                 955      50      27
 
Bme1580I G_kGCm'C

Cuts at:      0     222     969    1032 
   Size:        222     747      63

  Fragments arranged by size: 

                 747     222      63
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     103    1032 
   Size:        103     929
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     163    1032 
   Size:         70      93     869

  Fragments arranged by size: 

                 869      93      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     853    1032 
   Size:        853     179
 
BsaAI yAC'GTr

Cuts at:      0     630    1032 
   Size:        630     402
 
BsaBI GATnn'nnATC

Cuts at:      0     563    1032 
   Size:        563     469
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     965     995    1032 
   Size:        965      30      37

  Fragments arranged by size: 

                 965      37      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     973    1032 
   Size:        298     675      59

  Fragments arranged by size: 

                 675     298      59
 
BsiHKAI G_wGCw'C

Cuts at:      0      89     222    1032 
   Size:         89     133     810

  Fragments arranged by size: 

                 810     133      89
 
BsmI GAATG_Cn'

Cuts at:      0     193    1032 
   Size:        193     839
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     222     969    1032 
   Size:         89     133     747      63

  Fragments arranged by size: 

                 747     133      89      63
 
BsrDI GCAATG_nn'

Cuts at:      0     205     555    1032 
   Size:        205     350     477

  Fragments arranged by size: 

                 477     350     205
 
BstYI r'GATC_y

Cuts at:      0     425    1032 
   Size:        425     607
 
BtgI C'CryG_G

Cuts at:      0      11    1010    1032 
   Size:         11     999      22

  Fragments arranged by size: 

                 999      22      11
 
ClaI AT'CG_AT

Cuts at:      0     164    1032 
   Size:        164     868
 
DraI TTT'AAA

Cuts at:      0     621    1032 
   Size:        621     411
 
EarI CTCTTCn'nnn_

Cuts at:      0     299    1032 
   Size:        299     733
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     163     250     698    1032 
   Size:         70      93      87     448     334

  Fragments arranged by size: 

                 448     334      93      87      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     373    1032 
   Size:        373     659
 
EcoRI G'AATT_C

Cuts at:      0     683    1032 
   Size:        683     349
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     777     809    1032 
   Size:        777      32     223

  Fragments arranged by size: 

                 777     223      32
 
HindIII A'AGCT_T

Cuts at:      0     888    1032 
   Size:        888     144
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     102    1032 
   Size:        102     930
 
MspA1I CmG'CkG

Cuts at:      0      35    1032 
   Size:         35     997
 
NcoI C'CATG_G

Cuts at:      0    1010    1032 
   Size:       1010      22
 
NspI r_CATG'y

Cuts at:      0     717    1032 
   Size:        717     315
 
SfcI C'TryA_G

Cuts at:      0     740    1032 
   Size:        740     292
 
SmlI C'TyrA_G

Cuts at:      0     832    1032 
   Size:        832     200
 
SphI G_CATG'C

Cuts at:      0     717    1032 
   Size:        717     315
 
SspI AAT'ATT

Cuts at:      0     762     874    1032 
   Size:        762     112     158

  Fragments arranged by size: 

                 762     158     112
 
StyI C'CwwG_G

Cuts at:      0    1010    1032 
   Size:       1010      22
 
XbaI T'CTAG_A

Cuts at:      0     640    1032 
   Size:        640     392
 
XmnI GAAnn'nnTTC

Cuts at:      0      98     446     683     875    1032 
   Size:         98     348     237     192     157

  Fragments arranged by size: 

                 348     237     192     157      98
 

 Enzymes that do cut:

     AcuI     AhdI    ApaLI     ApoI     AvaI Bme1580I     BplI     BpmI
    BpuEI    BsaAI    BsaBI    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I
    BsrDI    BstYI     BtgI     ClaI     DraI     EarI  Eco57MI    EcoNI
    EcoRI    Hin4I  HindIII     MmeI   MspA1I     NcoI     NspI     SfcI
     SmlI     SphI     SspI     StyI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI