Restriction map for
pF1KB6417
(Linear) (Six-base) MAPSORT of: KIBB6417.seq Check: 4993 from: 1 to: 927
KIBB6417.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 440 927
Size: 440 487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 927
Size: 625 302
ApoI r'AATT_y
Cuts at: 0 751 927
Size: 751 176
AvaI C'yCGr_G
Cuts at: 0 307 927
Size: 307 620
BanI G'GyrC_C
Cuts at: 0 128 286 640 683 775 927
Size: 128 158 354 43 92 152
Fragments arranged by size:
354 158 152 128 92 43
BanII G_rGCy'C
Cuts at: 0 671 927
Size: 671 256
BbeI G_GCGC'C
Cuts at: 0 132 290 927
Size: 132 158 637
Fragments arranged by size:
637 158 132
BbvCI CC'TCA_GC
Cuts at: 0 17 927
Size: 17 910
Bme1580I G_kGCm'C
Cuts at: 0 780 927
Size: 780 147
BmgBI CAC'GTC
Cuts at: 0 339 927
Size: 339 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 250 367 755 927
Size: 8 242 117 388 172
Fragments arranged by size:
388 242 172 117 8
Bpu10I CC'TnA_GC
Cuts at: 0 17 512 927
Size: 17 495 415
Fragments arranged by size:
495 415 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 84 927
Size: 84 843
BsaBI GATnn'nnATC
Cuts at: 0 799 927
Size: 799 128
BsaHI Gr'CG_yC
Cuts at: 0 129 266 279 287 927
Size: 129 137 13 8 640
Fragments arranged by size:
640 137 129 13 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 72 208 382 421 927
Size: 72 136 174 39 506
Fragments arranged by size:
506 174 136 72 39
BseYI C'CCAG_C
Cuts at: 0 324 351 358 544 832 927
Size: 324 27 7 186 288 95
Fragments arranged by size:
324 288 186 95 27 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 861 927
Size: 583 278 66
Fragments arranged by size:
583 278 66
BsiEI CG_ry'CG
Cuts at: 0 12 173 927
Size: 12 161 754
Fragments arranged by size:
754 161 12
BsiHKAI G_wGCw'C
Cuts at: 0 671 927
Size: 671 256
Bsp1286I G_dGCh'C
Cuts at: 0 671 780 927
Size: 671 109 147
Fragments arranged by size:
671 147 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 728 927
Size: 728 199
BsrDI GCAATG_nn'
Cuts at: 0 821 927
Size: 821 106
BstYI r'GATC_y
Cuts at: 0 218 927
Size: 218 709
Bsu36I CC'TnA_GG
Cuts at: 0 841 927
Size: 841 86
BtgI C'CryG_G
Cuts at: 0 38 454 927
Size: 38 416 473
Fragments arranged by size:
473 416 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 641 927
Size: 641 286
BtsI GCAGTG_nn'
Cuts at: 0 453 927
Size: 453 474
DrdI GACnn_nn'nnGTC
Cuts at: 0 448 628 927
Size: 448 180 299
Fragments arranged by size:
448 299 180
EaeI y'GGCC_r
Cuts at: 0 170 431 927
Size: 170 261 496
Fragments arranged by size:
496 261 170
EagI C'GGCC_G
Cuts at: 0 170 927
Size: 170 757
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 205 927
Size: 205 722
EcoICRI GAG'CTC
Cuts at: 0 669 927
Size: 669 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 250 367 625 755 927
Size: 8 242 117 258 130 172
Fragments arranged by size:
258 242 172 130 117 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 907 927
Size: 907 20
EcoO109I rG'GnC_Cy
Cuts at: 0 52 355 927
Size: 52 303 572
Fragments arranged by size:
572 303 52
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 405 927
Size: 405 522
HaeII r_GCGC'y
Cuts at: 0 132 290 927
Size: 132 158 637
Fragments arranged by size:
637 158 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 582 614 927
Size: 582 32 313
Fragments arranged by size:
582 313 32
KasI G'GCGC_C
Cuts at: 0 128 286 927
Size: 128 158 641
Fragments arranged by size:
641 158 128
MslI CAynn'nnrTG
Cuts at: 0 121 927
Size: 121 806
MspA1I CmG'CkG
Cuts at: 0 436 927
Size: 436 491
NarI GG'CG_CC
Cuts at: 0 129 287 927
Size: 129 158 640
Fragments arranged by size:
640 158 129
NcoI C'CATG_G
Cuts at: 0 38 927
Size: 38 889
NdeI CA'TA_TG
Cuts at: 0 506 927
Size: 506 421
NspI r_CATG'y
Cuts at: 0 120 927
Size: 120 807
PasI CC'CwG_GG
Cuts at: 0 498 927
Size: 498 429
PflMI CCAn_nnn'nTGG
Cuts at: 0 887 927
Size: 887 40
PfoI T'CCnGG_A
Cuts at: 0 24 927
Size: 24 903
PpuMI rG'GwC_Cy
Cuts at: 0 52 355 927
Size: 52 303 572
Fragments arranged by size:
572 303 52
PspXI vC'TCGA_Gb
Cuts at: 0 307 927
Size: 307 620
PstI C_TGCA'G
Cuts at: 0 48 725 927
Size: 48 677 202
Fragments arranged by size:
677 202 48
SacI G_AGCT'C
Cuts at: 0 671 927
Size: 671 256
SanDI GG'GwC_CC
Cuts at: 0 355 927
Size: 355 572
ScaI AGT'ACT
Cuts at: 0 211 927
Size: 211 716
SfcI C'TryA_G
Cuts at: 0 44 293 437 721 927
Size: 44 249 144 284 206
Fragments arranged by size:
284 249 206 144 44
SfoI GGC'GCC
Cuts at: 0 130 288 927
Size: 130 158 639
Fragments arranged by size:
639 158 130
SmlI C'TyrA_G
Cuts at: 0 307 927
Size: 307 620
StuI AGG'CCT
Cuts at: 0 913 927
Size: 913 14
StyI C'CwwG_G
Cuts at: 0 38 908 927
Size: 38 870 19
Fragments arranged by size:
870 38 19
TatI w'GTAC_w
Cuts at: 0 209 806 927
Size: 209 597 121
Fragments arranged by size:
597 209 121
XhoI C'TCGA_G
Cuts at: 0 307 927
Size: 307 620
XmnI GAAnn'nnTTC
Cuts at: 0 826 927
Size: 826 101
Enzymes that do cut:
AccI AcuI ApoI AvaI BanI BanII BbeI BbvCI
Bme1580I BmgBI BpmI Bpu10I BsaI BsaBI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI
MslI MspA1I NarI NcoI NdeI NspI PasI PflMI
PfoI PpuMI PspXI PstI SacI SanDI ScaI SfcI
SfoI SmlI StuI StyI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
BpuEI BsaAI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII EarI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NaeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XcmI XmaI ZraI