Restriction map for
pF1KB6410
(Linear) (Six-base) MAPSORT of: KIBB6410.seq Check: 8177 from: 1 to: 1005
KIBB6410.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 399 1005
Size: 399 606
AatII G_ACGT'C
Cuts at: 0 605 1005
Size: 605 400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1005
Size: 523 482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 431 843 1005
Size: 431 412 162
Fragments arranged by size:
431 412 162
AlwNI CAG_nnn'CTG
Cuts at: 0 190 1005
Size: 190 815
ApaI G_GGCC'C
Cuts at: 0 238 1005
Size: 238 767
AvaI C'yCGr_G
Cuts at: 0 494 1005
Size: 494 511
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 952 985 1005
Size: 952 33 20
Fragments arranged by size:
952 33 20
BamHI G'GATC_C
Cuts at: 0 617 1005
Size: 617 388
BanI G'GyrC_C
Cuts at: 0 247 1005
Size: 247 758
BanII G_rGCy'C
Cuts at: 0 238 846 1005
Size: 238 608 159
Fragments arranged by size:
608 238 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 236 1005
Size: 202 34 769
Fragments arranged by size:
769 202 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 523 1005
Size: 523 482
Bme1580I G_kGCm'C
Cuts at: 0 238 252 1005
Size: 238 14 753
Fragments arranged by size:
753 238 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1005
Size: 383 622
BsaHI Gr'CG_yC
Cuts at: 0 602 1005
Size: 602 403
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 797 1005
Size: 797 208
BseYI C'CCAG_C
Cuts at: 0 12 290 908 1005
Size: 12 278 618 97
Fragments arranged by size:
618 278 97 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 609 1005
Size: 609 396
Bsp1286I G_dGCh'C
Cuts at: 0 238 252 846 1005
Size: 238 14 594 159
Fragments arranged by size:
594 238 159 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 399 1005
Size: 173 226 606
Fragments arranged by size:
606 226 173
BsrDI GCAATG_nn'
Cuts at: 0 218 722 1005
Size: 218 504 283
Fragments arranged by size:
504 283 218
BsrFI r'CCGG_y
Cuts at: 0 3 1005
Size: 3 1002
BstEII G'GTnAC_C
Cuts at: 0 990 1005
Size: 990 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 838 1005
Size: 838 167
BstYI r'GATC_y
Cuts at: 0 617 681 1005
Size: 617 64 324
Fragments arranged by size:
617 324 64
Bsu36I CC'TnA_GG
Cuts at: 0 677 1005
Size: 677 328
BtgI C'CryG_G
Cuts at: 0 82 743 1005
Size: 82 661 262
Fragments arranged by size:
661 262 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 904 1005
Size: 904 101
BtsI GCAGTG_nn'
Cuts at: 0 473 1005
Size: 473 532
ClaI AT'CG_AT
Cuts at: 0 458 1005
Size: 458 547
DraIII CAC_nnn'GTG
Cuts at: 0 754 946 1005
Size: 754 192 59
Fragments arranged by size:
754 192 59
EaeI y'GGCC_r
Cuts at: 0 656 1005
Size: 656 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 102 1005
Size: 102 903
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 523 1005
Size: 383 140 482
Fragments arranged by size:
482 383 140
EcoNI CCTnn'n_nnAGG
Cuts at: 0 110 169 1005
Size: 110 59 836
Fragments arranged by size:
836 110 59
EcoO109I rG'GnC_Cy
Cuts at: 0 234 235 768 935 1005
Size: 234 1 533 167 70
Fragments arranged by size:
533 234 167 70 1
EcoRV GAT'ATC
Cuts at: 0 210 1005
Size: 210 795
HaeII r_GCGC'y
Cuts at: 0 32 1005
Size: 32 973
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 1005
Size: 140 32 833
Fragments arranged by size:
833 140 32
MscI TGG'CCA
Cuts at: 0 658 1005
Size: 658 347
MslI CAynn'nnrTG
Cuts at: 0 343 1005
Size: 343 662
MspA1I CmG'CkG
Cuts at: 0 84 294 1005
Size: 84 210 711
Fragments arranged by size:
711 210 84
NaeI GCC'GGC
Cuts at: 0 5 1005
Size: 5 1000
NcoI C'CATG_G
Cuts at: 0 743 1005
Size: 743 262
NgoMIV G'CCGG_C
Cuts at: 0 3 1005
Size: 3 1002
PfoI T'CCnGG_A
Cuts at: 0 525 1005
Size: 525 480
PpuMI rG'GwC_Cy
Cuts at: 0 935 1005
Size: 935 70
PshAI GACnn'nnGTC
Cuts at: 0 94 521 1005
Size: 94 427 484
Fragments arranged by size:
484 427 94
PspOMI G'GGCC_C
Cuts at: 0 234 1005
Size: 234 771
PspXI vC'TCGA_Gb
Cuts at: 0 494 1005
Size: 494 511
PstI C_TGCA'G
Cuts at: 0 173 701 1005
Size: 173 528 304
Fragments arranged by size:
528 304 173
PvuII CAG'CTG
Cuts at: 0 294 1005
Size: 294 711
SacII CC_GC'GG
Cuts at: 0 85 1005
Size: 85 920
SbfI CC_TGCA'GG
Cuts at: 0 701 1005
Size: 701 304
SfcI C'TryA_G
Cuts at: 0 169 697 805 857 1005
Size: 169 528 108 52 148
Fragments arranged by size:
528 169 148 108 52
SmlI C'TyrA_G
Cuts at: 0 494 1005
Size: 494 511
StyI C'CwwG_G
Cuts at: 0 230 743 1005
Size: 230 513 262
Fragments arranged by size:
513 262 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 304 1005
Size: 235 69 701
Fragments arranged by size:
701 235 69
TatI w'GTAC_w
Cuts at: 0 670 1005
Size: 670 335
XhoI C'TCGA_G
Cuts at: 0 494 1005
Size: 494 511
ZraI GAC'GTC
Cuts at: 0 603 1005
Size: 603 402
Enzymes that do cut:
AarI AatII AcuI AlfI AlwNI ApaI AvaI BaeI
BamHI BanI BanII BcgI BciVI Bme1580I BpmI BsaHI
BseRI BseYI BsmBI Bsp1286I BspMI BsrDI BsrFI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
EaeI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
MscI MslI MspA1I NaeI NcoI NgoMIV PfoI PpuMI
PshAI PspOMI PspXI PstI PvuII SacII SbfI SfcI
SmlI StyI TaqII TatI XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BbeI BbsI BbvCI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I CspCI DraI DrdI EagI EarI EcoICRI EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PsrI PvuI RsrII SacI SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XmaI XmnI