Restriction map for
pF1KB6407
(Linear) (Six-base) MAPSORT of: KIBB6407.seq Check: 7415 from: 1 to: 918
KIBB6407.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 0 506 918
Size: 506 412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 918
Size: 892 26
AfeI AGC'GCT
Cuts at: 0 706 918
Size: 706 212
AleI CACnn'nnGTG
Cuts at: 0 778 918
Size: 778 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 796 918
Size: 796 122
ApoI r'AATT_y
Cuts at: 0 130 918
Size: 130 788
AvaI C'yCGr_G
Cuts at: 0 61 638 918
Size: 61 577 280
Fragments arranged by size:
577 280 61
BamHI G'GATC_C
Cuts at: 0 44 918
Size: 44 874
BanI G'GyrC_C
Cuts at: 0 780 918
Size: 780 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 42 76 918
Size: 42 34 842
Fragments arranged by size:
842 42 34
BglII A'GATC_T
Cuts at: 0 288 907 918
Size: 288 619 11
Fragments arranged by size:
619 288 11
BlpI GC'TnA_GC
Cuts at: 0 541 769 918
Size: 541 228 149
Fragments arranged by size:
541 228 149
Bme1580I G_kGCm'C
Cuts at: 0 785 918
Size: 785 133
BmgBI CAC'GTC
Cuts at: 0 565 918
Size: 565 353
BmrI ACTGGGnnnn_n'
Cuts at: 0 395 918
Size: 395 523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 541 918
Size: 541 377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 304 721 918
Size: 145 159 417 197
Fragments arranged by size:
417 197 159 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 918
Size: 313 605
BsaAI yAC'GTr
Cuts at: 0 39 575 918
Size: 39 536 343
Fragments arranged by size:
536 343 39
BsaHI Gr'CG_yC
Cuts at: 0 57 503 918
Size: 57 446 415
Fragments arranged by size:
446 415 57
BsaWI w'CCGG_w
Cuts at: 0 47 918
Size: 47 871
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 247 277 588 618 918
Size: 247 30 311 30 300
Fragments arranged by size:
311 300 247 30 30
BsiHKAI G_wGCw'C
Cuts at: 0 775 917 918
Size: 775 142 1
Fragments arranged by size:
775 142 1
BsmBI CGTCTCn'nnnn_
Cuts at: 0 635 918
Size: 635 283
Bsp1286I G_dGCh'C
Cuts at: 0 775 785 917 918
Size: 775 10 132 1
Fragments arranged by size:
775 132 10 1
BspEI T'CCGG_A
Cuts at: 0 47 918
Size: 47 871
BsrBI CCG'CTC
Cuts at: 0 756 918
Size: 756 162
BsrGI T'GTAC_A
Cuts at: 0 450 918
Size: 450 468
BssSI C'ACGA_G
Cuts at: 0 700 918
Size: 700 218
BstYI r'GATC_y
Cuts at: 0 44 288 907 918
Size: 44 244 619 11
Fragments arranged by size:
619 244 44 11
BtgI C'CryG_G
Cuts at: 0 776 918
Size: 776 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 90 918
Size: 90 828
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 372 407 918
Size: 372 35 511
Fragments arranged by size:
511 372 35
EaeI y'GGCC_r
Cuts at: 0 380 535 683 918
Size: 380 155 148 235
Fragments arranged by size:
380 235 155 148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 304 721 892 918
Size: 145 159 417 171 26
Fragments arranged by size:
417 171 159 145 26
HaeII r_GCGC'y
Cuts at: 0 548 708 800 918
Size: 548 160 92 118
Fragments arranged by size:
548 160 118 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 420 452 918
Size: 247 32 141 32 466
Fragments arranged by size:
466 247 141 32 32
HindIII A'AGCT_T
Cuts at: 0 98 874 918
Size: 98 776 44
Fragments arranged by size:
776 98 44
MscI TGG'CCA
Cuts at: 0 382 685 918
Size: 382 303 233
Fragments arranged by size:
382 303 233
MslI CAynn'nnrTG
Cuts at: 0 778 918
Size: 778 140
NcoI C'CATG_G
Cuts at: 0 776 918
Size: 776 142
NdeI CA'TA_TG
Cuts at: 0 480 918
Size: 480 438
NspI r_CATG'y
Cuts at: 0 235 918
Size: 235 683
ScaI AGT'ACT
Cuts at: 0 317 918
Size: 317 601
SfcI C'TryA_G
Cuts at: 0 712 903 918
Size: 712 191 15
Fragments arranged by size:
712 191 15
SmlI C'TyrA_G
Cuts at: 0 292 918
Size: 292 626
StyI C'CwwG_G
Cuts at: 0 360 680 776 918
Size: 360 320 96 142
Fragments arranged by size:
360 320 142 96
TatI w'GTAC_w
Cuts at: 0 315 450 918
Size: 315 135 468
Fragments arranged by size:
468 315 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 588 620 918
Size: 588 32 298
Fragments arranged by size:
588 298 32
ZraI GAC'GTC
Cuts at: 0 504 918
Size: 504 414
Enzymes that do cut:
AatII AcuI AfeI AleI AlfI ApoI AvaI BamHI
BanI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BsiHKAI BsmBI
Bsp1286I BspEI BsrBI BsrGI BssSI BstYI BtgI BtgZI
CspCI EaeI Eco57MI HaeII Hin4I HindIII MscI MslI
NcoI NdeI NspI ScaI SfcI SmlI StyI TatI
TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BmtI Bpu10I BsaI BsaBI BseRI BseYI BsgI
BsiEI BsiWI BsmI BspHI BspMI BsrDI BsrFI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI