Restriction map for
pF1KB6394
(Linear) (Six-base) MAPSORT of: KIBB6394.seq Check: 9035 from: 1 to: 909
KIBB6394.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 517 909
Size: 517 392
Acc65I G'GTAC_C
Cuts at: 0 400 819 909
Size: 400 419 90
Fragments arranged by size:
419 400 90
AflIII A'CryG_T
Cuts at: 0 410 909
Size: 410 499
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 625 657 909
Size: 625 32 252
Fragments arranged by size:
625 252 32
AlwNI CAG_nnn'CTG
Cuts at: 0 434 909
Size: 434 475
BanI G'GyrC_C
Cuts at: 0 33 400 819 909
Size: 33 367 419 90
Fragments arranged by size:
419 367 90 33
BanII G_rGCy'C
Cuts at: 0 113 909
Size: 113 796
BbeI G_GCGC'C
Cuts at: 0 37 909
Size: 37 872
BglI GCCn_nnn'nGGC
Cuts at: 0 10 909
Size: 10 899
BlpI GC'TnA_GC
Cuts at: 0 902 909
Size: 902 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 732 735 909
Size: 732 3 174
Fragments arranged by size:
732 174 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 533 623 909
Size: 136 397 90 286
Fragments arranged by size:
397 286 136 90
Bpu10I CC'TnA_GC
Cuts at: 0 14 909
Size: 14 895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 909
Size: 403 506
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 720 877 909
Size: 379 341 157 32
Fragments arranged by size:
379 341 157 32
BsaHI Gr'CG_yC
Cuts at: 0 34 909
Size: 34 875
BsaWI w'CCGG_w
Cuts at: 0 242 909
Size: 242 667
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 268 338 708 724 909
Size: 265 3 70 370 16 185
Fragments arranged by size:
370 265 185 70 16 3
BseYI C'CCAG_C
Cuts at: 0 120 909
Size: 120 789
BsiHKAI G_wGCw'C
Cuts at: 0 113 909
Size: 113 796
BsmI GAATG_Cn'
Cuts at: 0 507 909
Size: 507 402
Bsp1286I G_dGCh'C
Cuts at: 0 113 909
Size: 113 796
BspEI T'CCGG_A
Cuts at: 0 242 909
Size: 242 667
BspHI T'CATG_A
Cuts at: 0 828 909
Size: 828 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 517 909
Size: 517 392
BsrDI GCAATG_nn'
Cuts at: 0 137 909
Size: 137 772
BsrFI r'CCGG_y
Cuts at: 0 70 909
Size: 70 839
BssSI C'ACGA_G
Cuts at: 0 215 909
Size: 215 694
BstAPI GCAn_nnn'nTGC
Cuts at: 0 378 909
Size: 378 531
BstYI r'GATC_y
Cuts at: 0 454 909
Size: 454 455
Bsu36I CC'TnA_GG
Cuts at: 0 707 909
Size: 707 202
BtgI C'CryG_G
Cuts at: 0 80 909
Size: 80 829
BtsI GCAGTG_nn'
Cuts at: 0 579 909
Size: 579 330
DraIII CAC_nnn'GTG
Cuts at: 0 378 909
Size: 378 531
EaeI y'GGCC_r
Cuts at: 0 59 909
Size: 59 850
EcoICRI GAG'CTC
Cuts at: 0 111 909
Size: 111 798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 533 623 909
Size: 136 397 90 286
Fragments arranged by size:
397 286 136 90
EcoO109I rG'GnC_Cy
Cuts at: 0 855 909
Size: 855 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 903 909
Size: 903 6
HaeII r_GCGC'y
Cuts at: 0 37 909
Size: 37 872
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 443 446 475 478 909
Size: 443 3 29 3 431
Fragments arranged by size:
443 431 29 3 3
HindIII A'AGCT_T
Cuts at: 0 654 909
Size: 654 255
KasI G'GCGC_C
Cuts at: 0 33 909
Size: 33 876
KpnI G_GTAC'C
Cuts at: 0 404 823 909
Size: 404 419 86
Fragments arranged by size:
419 404 86
MfeI C'AATT_G
Cuts at: 0 588 909
Size: 588 321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 909
Size: 134 775
MscI TGG'CCA
Cuts at: 0 61 909
Size: 61 848
MslI CAynn'nnrTG
Cuts at: 0 812 909
Size: 812 97
NarI GG'CG_CC
Cuts at: 0 34 909
Size: 34 875
NspI r_CATG'y
Cuts at: 0 414 909
Size: 414 495
PciI A'CATG_T
Cuts at: 0 410 909
Size: 410 499
PfoI T'CCnGG_A
Cuts at: 0 113 433 909
Size: 113 320 476
Fragments arranged by size:
476 320 113
PpuMI rG'GwC_Cy
Cuts at: 0 855 909
Size: 855 54
PstI C_TGCA'G
Cuts at: 0 50 188 574 750 909
Size: 50 138 386 176 159
Fragments arranged by size:
386 176 159 138 50
SacI G_AGCT'C
Cuts at: 0 113 909
Size: 113 796
SfcI C'TryA_G
Cuts at: 0 46 184 570 746 909
Size: 46 138 386 176 163
Fragments arranged by size:
386 176 163 138 46
SfoI GGC'GCC
Cuts at: 0 35 909
Size: 35 874
SgrAI Cr'CCGG_yG
Cuts at: 0 70 909
Size: 70 839
SmlI C'TyrA_G
Cuts at: 0 382 909
Size: 382 527
StyI C'CwwG_G
Cuts at: 0 842 909
Size: 842 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 576 608 758 790 909
Size: 576 32 150 32 119
Fragments arranged by size:
576 150 119 32 32
Enzymes that do cut:
AarI Acc65I AflIII AloI AlwNI BanI BanII BbeI
BglI BlpI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstYI Bsu36I BtgI
BtsI DraIII EaeI EcoICRI Eco57MI EcoO109I FalI HaeII
Hin4I HindIII KasI KpnI MfeI MmeI MscI MslI
NarI NspI PciI PfoI PpuMI PstI SacI SfcI
SfoI SgrAI SmlI StyI TstI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI BsaAI
BsaBI BsaXI BsgI BsiEI BsiWI BsmBI BsrBI BsrGI
BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EarI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HpaI MluI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI