(Linear) (Six-base) MAPSORT of: KIBB6391.seq  Check: 6311  from: 1  to: 828



KIBB6391.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     727     828 
   Size:        518     209     101

  Fragments arranged by size: 

                 518     209     101
 
AleI CACnn'nnGTG

Cuts at:      0     261     828 
   Size:        261     567
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     221     253     828 
   Size:        221      32     575

  Fragments arranged by size: 

                 575     221      32
 
ApoI r'AATT_y

Cuts at:      0      91     193     342     828 
   Size:         91     102     149     486

  Fragments arranged by size: 

                 486     149     102      91
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     694     828 
   Size:        694     134
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     223     828 
   Size:        223     605
 
BfrBI ATG'CAT

Cuts at:      0     374     828 
   Size:        374     454
 
BglI GCCn_nnn'nGGC

Cuts at:      0     310     828 
   Size:        310     518
 
BmtI G_CTAG'C

Cuts at:      0     317     472     828 
   Size:        317     155     356

  Fragments arranged by size: 

                 356     317     155
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     314     365     653     752     828 
   Size:         52     262      51     288      99      76

  Fragments arranged by size: 

                 288     262      99      76      52      51
 
BsaWI w'CCGG_w

Cuts at:      0     278     828 
   Size:        278     550
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     652     828 
   Size:        652     176
 
BseYI C'CCAG_C

Cuts at:      0       7     828 
   Size:          7     821
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     828 
   Size:        438     390
 
BsrDI GCAATG_nn'

Cuts at:      0     323     828 
   Size:        323     505
 
BsrGI T'GTAC_A

Cuts at:      0     486     828 
   Size:        486     342
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     196     828 
   Size:        196     632
 
BstYI r'GATC_y

Cuts at:      0     249     828 
   Size:        249     579
 
BtgI C'CryG_G

Cuts at:      0      20     828 
   Size:         20     808
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     696     731     828 
   Size:        696      35      97

  Fragments arranged by size: 

                 696      97      35
 
DraI TTT'AAA

Cuts at:      0     479     542     828 
   Size:        479      63     286

  Fragments arranged by size: 

                 479     286      63
 
EarI CTCTTCn'nnn_

Cuts at:      0      72     293     828 
   Size:         72     221     535

  Fragments arranged by size: 

                 535     221      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     314     365     518     653     727     752     828 
   Size:         52     262      51     153     135      74      25      76

  Fragments arranged by size: 

                 262     153     135      76      74      52      51      25
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     224     828 
   Size:        224     604
 
EcoRI G'AATT_C

Cuts at:      0      91     828 
   Size:         91     737
 
HincII GTy'rAC

Cuts at:      0     111     624     828 
   Size:        111     513     204

  Fragments arranged by size: 

                 513     204     111
 
HindIII A'AGCT_T

Cuts at:      0     388     828 
   Size:        388     440
 
MslI CAynn'nnrTG

Cuts at:      0     261     828 
   Size:        261     567
 
NheI G'CTAG_C

Cuts at:      0     313     468     828 
   Size:        313     155     360

  Fragments arranged by size: 

                 360     313     155
 
NsiI A_TGCA'T

Cuts at:      0     376     828 
   Size:        376     452
 
PfoI T'CCnGG_A

Cuts at:      0     291     381     828 
   Size:        291      90     447

  Fragments arranged by size: 

                 447     291      90
 
SexAI A'CCwGG_T

Cuts at:      0     711     828 
   Size:        711     117
 
SfcI C'TryA_G

Cuts at:      0     167     828 
   Size:        167     661
 
SspI AAT'ATT

Cuts at:      0     564     828 
   Size:        564     264
 
TatI w'GTAC_w

Cuts at:      0     486     828 
   Size:        486     342
 

 Enzymes that do cut:

     AcuI     AleI     AloI     ApoI     BbsI    BciVI    BfrBI     BglI
     BmtI     BpmI    BsaWI    BseRI    BseYI     BsgI    BsrDI    BsrGI
   BstAPI    BstYI     BtgI    CspCI     DraI     EarI  Eco57MI    EcoNI
    EcoRI   HincII  HindIII     MslI     NheI     NsiI     PfoI    SexAI
     SfcI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI