Restriction map for
pF1KB6390
(Linear) (Six-base) MAPSORT of: KIBB6390.seq Check: 5737 from: 1 to: 903
KIBB6390.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 903
Size: 793 110
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 189 221 903
Size: 189 32 682
Fragments arranged by size:
682 189 32
ApaI G_GGCC'C
Cuts at: 0 39 99 286 903
Size: 39 60 187 617
Fragments arranged by size:
617 187 60 39
AvaI C'yCGr_G
Cuts at: 0 181 267 903
Size: 181 86 636
Fragments arranged by size:
636 181 86
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 805 838 903
Size: 805 33 65
Fragments arranged by size:
805 65 33
BanI G'GyrC_C
Cuts at: 0 46 250 903
Size: 46 204 653
Fragments arranged by size:
653 204 46
BanII G_rGCy'C
Cuts at: 0 39 60 99 286 903
Size: 39 21 39 187 617
Fragments arranged by size:
617 187 39 39 21
BbeI G_GCGC'C
Cuts at: 0 50 254 903
Size: 50 204 649
Fragments arranged by size:
649 204 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 886 903
Size: 886 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 71 105 903
Size: 71 34 798
Fragments arranged by size:
798 71 34
BclI T'GATC_A
Cuts at: 0 590 903
Size: 590 313
BglI GCCn_nnn'nGGC
Cuts at: 0 55 94 281 903
Size: 55 39 187 622
Fragments arranged by size:
622 187 55 39
BglII A'GATC_T
Cuts at: 0 561 903
Size: 561 342
Bme1580I G_kGCm'C
Cuts at: 0 39 99 116 286 903
Size: 39 60 17 170 617
Fragments arranged by size:
617 170 60 39 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 358 477 903
Size: 358 119 426
Fragments arranged by size:
426 358 119
BmtI G_CTAG'C
Cuts at: 0 7 903
Size: 7 896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 761 903
Size: 165 596 142
Fragments arranged by size:
596 165 142
BsaHI Gr'CG_yC
Cuts at: 0 47 251 903
Size: 47 204 652
Fragments arranged by size:
652 204 47
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 138 168 903
Size: 138 30 735
Fragments arranged by size:
735 138 30
BseYI C'CCAG_C
Cuts at: 0 92 298 903
Size: 92 206 605
Fragments arranged by size:
605 206 92
BsiEI CG_ry'CG
Cuts at: 0 73 161 242 903
Size: 73 88 81 661
Fragments arranged by size:
661 88 81 73
BsiHKAI G_wGCw'C
Cuts at: 0 295 501 903
Size: 295 206 402
Fragments arranged by size:
402 295 206
Bsp1286I G_dGCh'C
Cuts at: 0 39 60 99 116 286 295 501 903
Size: 39 21 39 17 170 9 206 402
Fragments arranged by size:
402 206 170 39 39 21 17 9
BsrBI CCG'CTC
Cuts at: 0 163 247 903
Size: 163 84 656
Fragments arranged by size:
656 163 84
BssHII G'CGCG_C
Cuts at: 0 19 903
Size: 19 884
BssSI C'ACGA_G
Cuts at: 0 27 903
Size: 27 876
BstYI r'GATC_y
Cuts at: 0 561 903
Size: 561 342
BtgI C'CryG_G
Cuts at: 0 316 903
Size: 316 587
BtsI GCAGTG_nn'
Cuts at: 0 695 903
Size: 695 208
EaeI y'GGCC_r
Cuts at: 0 70 134 158 189 239 273 873 903
Size: 70 64 24 31 50 34 600 30
Fragments arranged by size:
600 70 64 50 34 31 30 24
EagI C'GGCC_G
Cuts at: 0 70 158 239 903
Size: 70 88 81 664
Fragments arranged by size:
664 88 81 70
EarI CTCTTCn'nnn_
Cuts at: 0 653 903
Size: 653 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 761 793 903
Size: 165 596 32 110
Fragments arranged by size:
596 165 110 32
EcoO109I rG'GnC_Cy
Cuts at: 0 35 36 96 282 903
Size: 35 1 60 186 621
Fragments arranged by size:
621 186 60 35 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 503 903
Size: 503 400
HaeII r_GCGC'y
Cuts at: 0 50 254 903
Size: 50 204 649
Fragments arranged by size:
649 204 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 138 170 903
Size: 138 32 733
Fragments arranged by size:
733 138 32
KasI G'GCGC_C
Cuts at: 0 46 250 903
Size: 46 204 653
Fragments arranged by size:
653 204 46
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 676 903
Size: 425 251 227
Fragments arranged by size:
425 251 227
MspA1I CmG'CkG
Cuts at: 0 195 238 256 844 903
Size: 195 43 18 588 59
Fragments arranged by size:
588 195 59 43 18
NarI GG'CG_CC
Cuts at: 0 47 251 903
Size: 47 204 652
Fragments arranged by size:
652 204 47
NcoI C'CATG_G
Cuts at: 0 316 903
Size: 316 587
NheI G'CTAG_C
Cuts at: 0 3 903
Size: 3 900
PfoI T'CCnGG_A
Cuts at: 0 60 903
Size: 60 843
PspOMI G'GGCC_C
Cuts at: 0 35 95 282 903
Size: 35 60 187 621
Fragments arranged by size:
621 187 60 35
PvuII CAG'CTG
Cuts at: 0 844 903
Size: 844 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 281 903
Size: 281 622
SfoI GGC'GCC
Cuts at: 0 48 252 903
Size: 48 204 651
Fragments arranged by size:
651 204 48
SmaI CCC'GGG
Cuts at: 0 183 269 903
Size: 183 86 634
Fragments arranged by size:
634 183 86
SspI AAT'ATT
Cuts at: 0 402 903
Size: 402 501
StyI C'CwwG_G
Cuts at: 0 316 903
Size: 316 587
XmaI C'CCGG_G
Cuts at: 0 181 267 903
Size: 181 86 636
Fragments arranged by size:
636 181 86
Enzymes that do cut:
AcuI AloI ApaI AvaI BaeI BanI BanII BbeI
BbsI BcgI BclI BglI BglII Bme1580I BmrI BmtI
BpmI BsaHI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BsrBI
BssHII BssSI BstYI BtgI BtsI EaeI EagI EarI
Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MspA1I
NarI NcoI NheI PfoI PspOMI PvuII SfiI SfoI
SmaI SspI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BamHI BbvCI BciVI BfrBI BlpI
BmgBI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI MslI NaeI
NdeI NgoMIV NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI