Restriction map for
pF1KB6379
(Linear) (Six-base) MAPSORT of: KIBB6379.seq Check: 1445 from: 1 to: 684
KIBB6379.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 310 684
Size: 310 374
AatII G_ACGT'C
Cuts at: 0 20 684
Size: 20 664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 684
Size: 328 356
AflII C'TTAA_G
Cuts at: 0 108 360 684
Size: 108 252 324
Fragments arranged by size:
324 252 108
AlwNI CAG_nnn'CTG
Cuts at: 0 180 684
Size: 180 504
ApoI r'AATT_y
Cuts at: 0 52 148 526 684
Size: 52 96 378 158
Fragments arranged by size:
378 158 96 52
BanI G'GyrC_C
Cuts at: 0 544 684
Size: 544 140
BglII A'GATC_T
Cuts at: 0 354 684
Size: 354 330
BlpI GC'TnA_GC
Cuts at: 0 265 684
Size: 265 419
Bme1580I G_kGCm'C
Cuts at: 0 547 684
Size: 547 137
BmgBI CAC'GTC
Cuts at: 0 24 684
Size: 24 660
BmtI G_CTAG'C
Cuts at: 0 628 684
Size: 628 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 373 684
Size: 373 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 202 217 684
Size: 82 120 15 467
Fragments arranged by size:
467 120 82 15
BsaHI Gr'CG_yC
Cuts at: 0 17 684
Size: 17 667
BsaWI w'CCGG_w
Cuts at: 0 26 684
Size: 26 658
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 215 245 684
Size: 215 30 439
Fragments arranged by size:
439 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 365 484 684
Size: 365 119 200
Fragments arranged by size:
365 200 119
BseYI C'CCAG_C
Cuts at: 0 449 555 684
Size: 449 106 129
Fragments arranged by size:
449 129 106
BsiHKAI G_wGCw'C
Cuts at: 0 117 684
Size: 117 567
Bsp1286I G_dGCh'C
Cuts at: 0 117 547 684
Size: 117 430 137
Fragments arranged by size:
430 137 117
BspEI T'CCGG_A
Cuts at: 0 26 684
Size: 26 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 310 444 684
Size: 310 134 240
Fragments arranged by size:
310 240 134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 555 684
Size: 555 129
BstYI r'GATC_y
Cuts at: 0 29 354 684
Size: 29 325 330
Fragments arranged by size:
330 325 29
DrdI GACnn_nn'nnGTC
Cuts at: 0 22 684
Size: 22 662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 202 217 328 684
Size: 82 120 15 111 356
Fragments arranged by size:
356 120 111 82 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 282 684
Size: 282 402
EcoO109I rG'GnC_Cy
Cuts at: 0 103 177 684
Size: 103 74 507
Fragments arranged by size:
507 103 74
EcoRV GAT'ATC
Cuts at: 0 87 684
Size: 87 597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 355 684
Size: 355 329
HaeII r_GCGC'y
Cuts at: 0 76 684
Size: 76 608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 684
Size: 196 32 456
Fragments arranged by size:
456 196 32
HindIII A'AGCT_T
Cuts at: 0 565 604 684
Size: 565 39 80
Fragments arranged by size:
565 80 39
MspA1I CmG'CkG
Cuts at: 0 555 684
Size: 555 129
NheI G'CTAG_C
Cuts at: 0 624 684
Size: 624 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 684
Size: 240 444
PfoI T'CCnGG_A
Cuts at: 0 233 509 684
Size: 233 276 175
Fragments arranged by size:
276 233 175
PpuMI rG'GwC_Cy
Cuts at: 0 103 177 684
Size: 103 74 507
Fragments arranged by size:
507 103 74
PvuII CAG'CTG
Cuts at: 0 555 684
Size: 555 129
SmlI C'TyrA_G
Cuts at: 0 108 360 684
Size: 108 252 324
Fragments arranged by size:
324 252 108
StuI AGG'CCT
Cuts at: 0 277 684
Size: 277 407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 684
Size: 491 32 161
Fragments arranged by size:
491 161 32
XbaI T'CTAG_A
Cuts at: 0 324 684
Size: 324 360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 387 684
Size: 387 297
XmnI GAAnn'nnTTC
Cuts at: 0 341 684
Size: 341 343
ZraI GAC'GTC
Cuts at: 0 18 684
Size: 18 666
Enzymes that do cut:
AarI AatII AcuI AflII AlwNI ApoI BanI BglII
BlpI Bme1580I BmgBI BmtI BplI BpmI BsaHI BsaWI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BstXI
BstYI DrdI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HindIII MspA1I NheI PflMI PfoI PpuMI PvuII
SmlI StuI TstI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BmrI Bpu10I BpuEI BsaI
BsaAI BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EagI EarI EciI EcoICRI EcoRI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TatI Tth111I XhoI XmaI