Restriction map for
pF1KB6376
(Linear) (Six-base) MAPSORT of: KIBB6376.seq Check: 7323 from: 1 to: 897
KIBB6376.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 595 897
Size: 412 183 302
Fragments arranged by size:
412 302 183
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 532 564 897
Size: 532 32 333
Fragments arranged by size:
532 333 32
AlwNI CAG_nnn'CTG
Cuts at: 0 331 888 897
Size: 331 557 9
Fragments arranged by size:
557 331 9
ApaI G_GGCC'C
Cuts at: 0 149 862 897
Size: 149 713 35
Fragments arranged by size:
713 149 35
ApoI r'AATT_y
Cuts at: 0 370 897
Size: 370 527
BanI G'GyrC_C
Cuts at: 0 868 897
Size: 868 29
BanII G_rGCy'C
Cuts at: 0 149 862 897
Size: 149 713 35
Fragments arranged by size:
713 149 35
BbvCI CC'TCA_GC
Cuts at: 0 11 284 897
Size: 11 273 613
Fragments arranged by size:
613 273 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 598 632 897
Size: 598 34 265
Fragments arranged by size:
598 265 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 605 897
Size: 605 292
BclI T'GATC_A
Cuts at: 0 890 897
Size: 890 7
Bme1580I G_kGCm'C
Cuts at: 0 149 862 897
Size: 149 713 35
Fragments arranged by size:
713 149 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 617 827 897
Size: 420 197 210 70
Fragments arranged by size:
420 210 197 70
Bpu10I CC'TnA_GC
Cuts at: 0 11 284 897
Size: 11 273 613
Fragments arranged by size:
613 273 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 697 897
Size: 697 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 897
Size: 532 30 335
Fragments arranged by size:
532 335 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 897
Size: 70 827
BseYI C'CCAG_C
Cuts at: 0 105 897
Size: 105 792
BsiHKAI G_wGCw'C
Cuts at: 0 75 897
Size: 75 822
Bsp1286I G_dGCh'C
Cuts at: 0 75 149 862 897
Size: 75 74 713 35
Fragments arranged by size:
713 75 74 35
BsrDI GCAATG_nn'
Cuts at: 0 275 897
Size: 275 622
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 897
Size: 468 429
BstYI r'GATC_y
Cuts at: 0 756 897
Size: 756 141
BtgI C'CryG_G
Cuts at: 0 125 467 897
Size: 125 342 430
Fragments arranged by size:
430 342 125
BtsI GCAGTG_nn'
Cuts at: 0 170 248 354 897
Size: 170 78 106 543
Fragments arranged by size:
543 170 106 78
DraIII CAC_nnn'GTG
Cuts at: 0 303 400 868 897
Size: 303 97 468 29
Fragments arranged by size:
468 303 97 29
EaeI y'GGCC_r
Cuts at: 0 2 897
Size: 2 895
EarI CTCTTCn'nnn_
Cuts at: 0 148 193 446 897
Size: 148 45 253 451
Fragments arranged by size:
451 253 148 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 420 595 617 827 897
Size: 412 8 175 22 210 70
Fragments arranged by size:
412 210 175 70 22 8
EcoO109I rG'GnC_Cy
Cuts at: 0 145 146 897
Size: 145 1 751
Fragments arranged by size:
751 145 1
EcoRI G'AATT_C
Cuts at: 0 370 897
Size: 370 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 897
Size: 343 32 522
Fragments arranged by size:
522 343 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 897
Size: 425 472
MspA1I CmG'CkG
Cuts at: 0 10 897
Size: 10 887
NcoI C'CATG_G
Cuts at: 0 467 897
Size: 467 430
NspI r_CATG'y
Cuts at: 0 444 897
Size: 444 453
PflMI CCAn_nnn'nTGG
Cuts at: 0 303 897
Size: 303 594
PfoI T'CCnGG_A
Cuts at: 0 212 897
Size: 212 685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 897
Size: 532 32 333
Fragments arranged by size:
532 333 32
PspOMI G'GGCC_C
Cuts at: 0 145 858 897
Size: 145 713 39
Fragments arranged by size:
713 145 39
PstI C_TGCA'G
Cuts at: 0 336 897
Size: 336 561
SapI GCTCTTCn'nnn_
Cuts at: 0 446 897
Size: 446 451
SexAI A'CCwGG_T
Cuts at: 0 299 864 897
Size: 299 565 33
Fragments arranged by size:
565 299 33
SfcI C'TryA_G
Cuts at: 0 21 332 897
Size: 21 311 565
Fragments arranged by size:
565 311 21
StyI C'CwwG_G
Cuts at: 0 461 467 551 897
Size: 461 6 84 346
Fragments arranged by size:
461 346 84 6
XmnI GAAnn'nnTTC
Cuts at: 0 208 897
Size: 208 689
Enzymes that do cut:
AcuI AloI AlwNI ApaI ApoI BanI BanII BbvCI
BcgI BciVI BclI Bme1580I BpmI Bpu10I BsaI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BsrDI BstXI BstYI BtgI
BtsI DraIII EaeI EarI Eco57MI EcoO109I EcoRI Hin4I
MmeI MspA1I NcoI NspI PflMI PfoI PpiI PspOMI
PstI SapI SexAI SfcI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI