(Linear) (Six-base) MAPSORT of: KIBB6357.seq  Check: 899  from: 1  to: 801



KIBB6357.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AflIII A'CryG_T

Cuts at:      0     401     801 
   Size:        401     400
 
ApoI r'AATT_y

Cuts at:      0     190     726     801 
   Size:        190     536      75

  Fragments arranged by size: 

                 536     190      75
 
AvaI C'yCGr_G

Cuts at:      0     350     801 
   Size:        350     451
 
BanII G_rGCy'C

Cuts at:      0     486     801 
   Size:        486     315
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     707     801 
   Size:        707      94
 
BmgBI CAC'GTC

Cuts at:      0     231     801 
   Size:        231     570
 
Bpu10I CC'TnA_GC

Cuts at:      0     104     801 
   Size:        104     697
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     160     801 
   Size:        100      60     641

  Fragments arranged by size: 

                 641     100      60
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     559     801 
   Size:        559     242
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     494     575     801 
   Size:        494      81     226

  Fragments arranged by size: 

                 494     226      81
 
BsiHKAI G_wGCw'C

Cuts at:      0     486     801 
   Size:        486     315
 
BsmI GAATG_Cn'

Cuts at:      0     758     801 
   Size:        758      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     486     801 
   Size:        486     315
 
BspHI T'CATG_A

Cuts at:      0     461     639     801 
   Size:        461     178     162

  Fragments arranged by size: 

                 461     178     162
 
BstEII G'GTnAC_C

Cuts at:      0     324     801 
   Size:        324     477
 
BstYI r'GATC_y

Cuts at:      0     416     717     801 
   Size:        416     301      84

  Fragments arranged by size: 

                 416     301      84
 
Bsu36I CC'TnA_GG

Cuts at:      0      62     801 
   Size:         62     739
 
BtsI GCAGTG_nn'

Cuts at:      0     695     801 
   Size:        695     106
 
ClaI AT'CG_AT

Cuts at:      0     378     801 
   Size:        378     423
 
DraI TTT'AAA

Cuts at:      0     398     732     801 
   Size:        398     334      69

  Fragments arranged by size: 

                 398     334      69
 
EaeI y'GGCC_r

Cuts at:      0     648     801 
   Size:        648     153
 
EcoICRI GAG'CTC

Cuts at:      0     484     801 
   Size:        484     317
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     548     801 
   Size:        548     253
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     111     113     143     145     801 
   Size:        111       2      30       2     656

  Fragments arranged by size: 

                 656     111      30       2       2
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     724     801 
   Size:        724      77
 
MscI TGG'CCA

Cuts at:      0     650     801 
   Size:        650     151
 
MslI CAynn'nnrTG

Cuts at:      0     575     801 
   Size:        575     226
 
MspA1I CmG'CkG

Cuts at:      0      57     801 
   Size:         57     744
 
NspI r_CATG'y

Cuts at:      0     405     801 
   Size:        405     396
 
PciI A'CATG_T

Cuts at:      0     401     801 
   Size:        401     400
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     508     657     706     801 
   Size:        508     149      49      95

  Fragments arranged by size: 

                 508     149      95      49
 
SacI G_AGCT'C

Cuts at:      0     486     801 
   Size:        486     315
 
SfcI C'TryA_G

Cuts at:      0      88     330     801 
   Size:         88     242     471

  Fragments arranged by size: 

                 471     242      88
 
SmlI C'TyrA_G

Cuts at:      0     115     175     350     801 
   Size:        115      60     175     451

  Fragments arranged by size: 

                 451     175     115      60
 
XhoI C'TCGA_G

Cuts at:      0     350     801 
   Size:        350     451
 
XmnI GAAnn'nnTTC

Cuts at:      0     786     801 
   Size:        786      15
 

 Enzymes that do cut:

   AflIII     ApoI     AvaI    BanII    BciVI    BmgBI   Bpu10I    BpuEI
     BsaI    BseRI  BsiHKAI     BsmI Bsp1286I    BspHI   BstEII    BstYI
   Bsu36I     BtsI     ClaI     DraI     EaeI  EcoICRI    EcoNI    Hin4I
     MmeI     MscI     MslI   MspA1I     NspI     PciI    PflMI     SacI
     SfcI     SmlI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I     BtgI    BtgZI    CspCI   DraIII     DrdI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI